abstract：:Technoeconomic analysis (TEA) is an approach for conducting process design and simulation, informed by empirical data, to estimate capital costs, operating costs, mass balances, and energy balances for a commercial scale biorefinery. TEA serves as a useful method to screen potential research priorities, identify cost ...

journal_title：Current opinion in biotechnology

authors： Scown CD,Baral NR,Yang M,Vora N,Huntington T

更新日期：2021-01-18 00:00:00

abstract：:Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding ...

journal_title：Current opinion in biotechnology

authors： Lynch JH,Huang XQ,Dudareva N

更新日期：2021-01-08 00:00:00

abstract：:The continuing expansion of protein and genome sequence databases is an opportunity to identify novel enzymes with biotechnological applications. Whether applied to enzymology, chemical biology, systems biology, and microbiology, database mining must be 'user-friendly' so that experimentalists can devise focused strat...

journal_title：Current opinion in biotechnology

authors： Zallot R,Oberg N,Gerlt JA

更新日期：2021-01-05 00:00:00

abstract：:Microbial cell factory performance is significantly affected by the cell morphology and membrane homeostasis. It is important to ensure that cell factories are able to regulate cell morphology and maintain membrane homeostasis. Cell morphology can be controlled by regulating the formation of elongasomes and divisomes,...

journal_title：Current opinion in biotechnology

authors： Guo L,Pang Z,Gao C,Chen X,Liu L

更新日期：2020-12-01 00:00:00

abstract：:Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...

journal_title：Current opinion in biotechnology

authors： Park D,Swayambhu G,Pfeifer BA

更新日期：2020-12-01 00:00:00

abstract：:New technologies have allowed researchers to better design, build, and analyze complex consortia. These developments are fueling a wider implementation of consortium-based bioprocessing by leveraging synthetic biology, delivering on the field's multitudinous promises of higher efficiencies, superior resiliency, augmen...

journal_title：Current opinion in biotechnology

authors： Rapp KM,Jenkins JP,Betenbaugh MJ

更新日期：2020-12-01 00:00:00

abstract：:Ultrasonographic imaging of ovarian morphology is used widely to inform reproductive health status in women. Metabolic disturbances induced by a negative energy balance (e.g. undernutrition) or positive energy balance (e.g. overnutrition, obesity) are known to drive or exacerbate reproductive dysfunction. Whether the ...

journal_title：Current opinion in biotechnology

authors： Vanden Brink H,Pea J,Lujan ME

更新日期：2020-11-25 00:00:00

abstract：:Modern agriculture and food production systems are facing increasing pressures from climate change, land and water availability, and, more recently, a pandemic. These factors are threatening the environmental and economic sustainability of current and future food supply systems. Scientific and technological innovation...

journal_title：Current opinion in biotechnology

authors： Jung J,Maeda M,Chang A,Bhandari M,Ashapure A,Landivar-Bowles J

更新日期：2020-10-07 00:00:00

abstract：:Plant-derived diterpenoids are indispensable to plant development, stress-resistance and interaction with environmental microorganisms. Besides significant roles in plant fitness and adaption, many bioactivities beneficial to human beings are also found in diterpenoids from terrestrial plants. However, these high-valu...

journal_title：Current opinion in biotechnology

authors： Shao J,Sun Y,Liu H,Wang Y

更新日期：2020-10-05 00:00:00

abstract：:Extremolytes are small organic molecules, which protect cells under extreme, virtually inhabitable conditions. Their exceptional properties can be translated into health-promoting and therapeutic activities, which open an avenue of opportunities for the cosmetic, medical, and food industries. Supported by powerful app...

journal_title：Current opinion in biotechnology

authors： Becker J,Wittmann C

更新日期：2020-10-01 00:00:00

abstract：:Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activiti...

journal_title：Current opinion in biotechnology

authors： Allen DK,Young JD

更新日期：2020-08-01 00:00:00

abstract：:Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

journal_title：Current opinion in biotechnology

authors： Kim NM,Sinnott RW,Sandoval NR

更新日期：2020-08-01 00:00:00

abstract：:Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically...

journal_title：Current opinion in biotechnology

authors： Prangemeier T,Lehr FX,Schoeman RM,Koeppl H

更新日期：2020-06-01 00:00:00

abstract：:Patient-derived xenografts (PDXs) are obtained by transplanting fragments of a patient's tumour into immunodeficient mice. Growth and propagation of PDXs allows correlating therapeutic response in vivo with extensive, multi-dimensional molecular annotation, leading to identification of predictive biomarkers. PDXs are ...

journal_title：Current opinion in biotechnology

authors： Invrea F,Rovito R,Torchiaro E,Petti C,Isella C,Medico E

更新日期：2020-06-01 00:00:00

abstract：:Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels. In the past decade, novel hydrocar...

journal_title：Current opinion in biotechnology

authors： Liu K,Li S

更新日期：2020-04-01 00:00:00

abstract：:Hydrogen is already a major commodity and process intermediate for fertilizer production, petroleum processing, and chemical synthesis. It also offers unrealized potential for energy storage. While biological production offers an expandable and sustainable source, enthusiasm has been dampened by slow research progress...

journal_title：Current opinion in biotechnology

authors： Swartz J

更新日期：2020-04-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:Microbial communities within anaerobic ecosystems have evolved to degrade and recycle carbon throughout the earth. A number of strains have been isolated from anaerobic microbial communities, which are rich in carbohydrate active enzymes (CAZymes) to liberate fermentable sugars from crude plant biomass (lignocellulose...

journal_title：Current opinion in biotechnology

authors： Lillington SP,Leggieri PA,Heom KA,O'Malley MA

更新日期：2020-04-01 00:00:00

abstract：:Silk combines biological properties, such as non-toxicity and biodegradability, with physico-chemical ones, for example, mechanical strength. Base on molecular engineering, nowadays also new non-silk functions can be implemented in silk materials. Driven by rational design and ingenuity, innovative recombinant silk pr...

journal_title：Current opinion in biotechnology

authors： Saric M,Scheibel T

更新日期：2019-12-01 00:00:00

abstract：:Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological conditions. Abs are required for the quantification of targets, dete...

journal_title：Current opinion in biotechnology

authors： Basu K,Green EM,Cheng Y,Craik CS

更新日期：2019-12-01 00:00:00

abstract：:Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...

journal_title：Current opinion in biotechnology

authors： Liu Y,Nielsen J

更新日期：2019-12-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:Synthetic biology efforts began in simple single-cell systems, which were relatively easy to manipulate genetically (Cameron et al., 2014). The field grew exponentially in the last two decades, and one of the latest frontiers are synthetic developmental programs for multicellular mammalian systems (Black et al., 2017;...

journal_title：Current opinion in biotechnology

authors： Santorelli M,Lam C,Morsut L

更新日期：2019-10-01 00:00:00

abstract：:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for preci...

journal_title：Current opinion in biotechnology

authors： Tolle F,Stücheli P,Fussenegger M

更新日期：2019-10-01 00:00:00

abstract：:Fluorescent proteins (FPs) and fluorescent protein-derived biosensors are indispensable tools in life sciences. They make it possible to probe the location, activity, or interaction of molecules of interest from a subcellular to a multicellular scale. The desire for high-resolution and multidimensional information imp...

journal_title：Current opinion in biotechnology

authors： Duwé S,Dedecker P

更新日期：2019-08-01 00:00:00

abstract：:RNA plays important roles in the regulation of gene expressions and other cellular functions. It functions as both as an informational carrier and a nanomachine due to its complementary base-pairing ability and complexed three-dimensional structure. Several nanostructures have been designed and constructed by exploiti...

journal_title：Current opinion in biotechnology

authors： Ohno H,Akamine S,Saito H

更新日期：2019-08-01 00:00:00

abstract：:Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...

journal_title：Current opinion in biotechnology

authors： Newton RJ,McClary JS

更新日期：2019-06-01 00:00:00

abstract：:Novel biotechnological approaches such as Metabolic Engineering (ME) and New Plant Breeding Techniques (NPBTs) are currently being developed to produce biological compounds for food and non-food products. NPBTs span a range of methods for in vivo production in crops, some of which are classified as GMOs while others a...

journal_title：Current opinion in biotechnology

authors： Pei L,Schmidt M

更新日期：2019-04-01 00:00:00

abstract：:Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...

journal_title：Current opinion in biotechnology

authors： Vanholme B,El Houari I,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

journal_title：Current opinion in biotechnology

authors： Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

更新日期：2019-04-01 00:00:00

abstract：:The pathway of monolignol biosynthesis involves many components interacting in a metabolic grid to regulate the supply and ratios of monolignols for lignification. The complexity of the pathway challenges any intuitive prediction of the output without mathematical modeling. Several models have been presented to quanti...

journal_title：Current opinion in biotechnology

authors： Wang JP,Matthews ML,Naik PP,Williams CM,Ducoste JJ,Sederoff RR,Chiang VL

更新日期：2019-04-01 00:00:00

abstract：:Reductive catalytic fractionation (RCF) of lignocellulose is an emerging biorefinery scheme that combines biomass fractionation with lignin depolymerisation. Central to this scheme is the integration of heterogeneous catalysis, which overcomes the tendency of lignin to repolymerise. Ultimately, this leads to a low-Mw ...

journal_title：Current opinion in biotechnology

authors： Renders T,Van den Bossche G,Vangeel T,Van Aelst K,Sels B

更新日期：2019-04-01 00:00:00

abstract：:Lignin is a principal structural component of cell walls in higher terrestrial plants. It reinforces the cell walls, facilitates water transport, and acts as a physical barrier to pathogens. Lignin is typically described as being composed of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) units that derive from th...

journal_title：Current opinion in biotechnology

authors： Vanholme R,De Meester B,Ralph J,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:Isothermal titration calorimetry is widely used to measure the affinities and enthalpies of interaction between proteins and/or small molecules. The quantitative nature of the technique is especially useful in the characterization of recombinant proteins while determining the fraction of protein capable of binding a s...

journal_title：Current opinion in biotechnology

authors： Baranauskiene L,Kuo TC,Chen WY,Matulis D

更新日期：2019-02-01 00:00:00

abstract：:Academic microfluidics has decisively shifted in recent years from the research on phenomenology and proof-of-concept fluidic functionalities to the developments oriented at applications with biology, medicine and biotechnology in prime focus. Significant efforts are made to demonstrate that microfluidics can be used ...

journal_title：Current opinion in biotechnology

authors： Scheler O,Postek W,Garstecki P

更新日期：2019-02-01 00:00:00

abstract：:Tumor-derived DNA, found in body fluids (liquid biopsy) of cancer patients as part of cell-free DNA (cfDNA), lends itself for noninvasive cancer detection and monitoring. Advantages of cfDNA as analytical target have evoked a burst of sophisticated techniques, providing clinically relevant information. Each cell type ...

journal_title：Current opinion in biotechnology

authors： Daniūnaitė K,Jarmalaitė S,Kriukienė E

更新日期：2019-02-01 00:00:00

abstract：:Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...

journal_title：Current opinion in biotechnology

authors： Bjork SM,Joensson HN

更新日期：2019-02-01 00:00:00

abstract：:Understanding the hierarchical structure of proteins at their fundamental length scales is essential to get insights into their functions and roles in fundamental biological processes. Near-field scanning optical microscopy (NSOM), which overcomes the diffraction limits of conventional optics, provides a powerful anal...

journal_title：Current opinion in biotechnology

authors： Yong YC,Wang YZ,Zhong JJ

更新日期：2018-12-01 00:00:00

abstract：:The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medi...

journal_title：Current opinion in biotechnology

authors： Schwechheimer SK,Becker J,Wittmann C

更新日期：2018-12-01 00:00:00

abstract：:Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xyl...

journal_title：Current opinion in biotechnology

authors： Yaguchi A,Spagnuolo M,Blenner M

更新日期：2018-10-01 00:00:00