Abstract：
:Fluorescent proteins (FPs) and fluorescent protein-derived biosensors are indispensable tools in life sciences. They make it possible to probe the location, activity, or interaction of molecules of interest from a subcellular to a multicellular scale. The desire for high-resolution and multidimensional information imposes continuously increasing demands on the performance of the employed probes leading to a continued need for optimization and integration of additional features, such as phototransformable behavior. This review highlights the latest advances in FP engineering, which increase throughput and tailor the improvements directly to the envisioned experiments. Additionally, we discuss recent alternative approaches to introduce or alter phototransformable behavior and describe selected applications of phototransformable behavior in biosensors.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Duwé S,Dedecker Pdoi
10.1016/j.copbio.2019.04.006subject
Has Abstractpub_date
2019-08-01 00:00:00pages
183-191eissn
0958-1669issn
1879-0429pii
S0958-1669(18)30231-3journal_volume
58pub_type
杂志文章,评审abstract：:One goal of plant genetic engineering is the manipulation of protein levels within crop plants. New insights into the ubiquitin-dependent proteolytic pathway provide potential novel ways of enhancing levels of desired proteins by synthesizing them as ubiquitin fusions, and reducing levels of undesired proteins by sele...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90144-8
更新日期：1992-04-01 00:00:00
abstract：:Recent advances in miniaturization and automation of technologies have enabled cell-based assay high-throughput screening, bringing along new challenges in data analysis. Automation, standardization, reproducibility have become requirements for qualitative research. The Bioconductor community has worked in that direct...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.09.003
更新日期：2013-02-01 00:00:00
abstract：:Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage. Given this situation, one would predict translational problems with an abundant...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(95)80082-4
更新日期：1995-10-01 00:00:00
abstract：:Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology can also ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(93)90071-4
更新日期：1993-10-01 00:00:00
abstract：:As the ambitions of synthetic biology approach genome-scale engineering, comprehensive characterization of cellular systems is required, as well as a means to accurately model cell-scale molecular interactions. These requirements are coincident with the goals of systems biology and, thus, systems biology will become t...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2006.08.001
更新日期：2006-10-01 00:00:00
abstract：:Poxviruses are widely used for the cytoplasmic expression of recombinant genes in mammalian cells. Recent improvements allow high expression and simplify the integration of multiple foreign genes. Vaccinia virus mutants and other poxviruses that undergo abortive infection in mammalian cells are receiving special atten...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(97)80031-6
更新日期：1997-10-01 00:00:00
abstract：:Life depends on the interaction of proteins. The availability of the complete human genome sequence has highlighted the need for a tool to analyse protein interactions and several databases have been compiled for this purpose. These databases document, categorize, and analyze interacting proteins and the cellular func...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00224-x
更新日期：2001-08-01 00:00:00
abstract：:The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(02)00331-2
更新日期：2002-08-01 00:00:00
abstract：:The design and construction of synthetic gene circuits with complex spatiotemporal dynamics was pioneered in bacteria, but it took almost a decade until synthetic biologists were able to construct genetic circuits with complex spatiotemporal dynamics in mammalian cells. This review highlights the most recent advances ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2010.07.006
更新日期：2010-10-01 00:00:00
abstract：:Fermentation of food components by microbes occurs both during certain food production processes and in the gastro-intestinal tract. In these processes specific compounds are produced that originate from either biotransformation reactions or biosynthesis, and that can affect the health of the consumer. In this review,...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2010.12.004
更新日期：2011-04-01 00:00:00
abstract：:Phage display of proteins continues to be an important technology with a variety of applications. In the past year, advances have been made in coupling rational protein design with the power of the display selection process. In addition to the widely used filamentous phage, other bacteriophage surface expression syste...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80060-7
更新日期：1996-10-01 00:00:00
abstract：:Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90167-h
更新日期：1992-08-01 00:00:00
abstract：:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(95)80006-9
更新日期：1995-02-01 00:00:00
abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2008.02.016
更新日期：2008-04-01 00:00:00
abstract：:The most significant feature of the current transgenic models of Alzheimer's disease continues to be the amyloid phenotype. In the past year, mice have been more extensively characterized in terms of the effect of amyloid accumulation on downstream events, such as neurodegeneration and behavioral changes, but the resu...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(98)80132-8
更新日期：1998-12-01 00:00:00
abstract：:The intestinal microbiota plays a profound role in human health and extensive research has been dedicated to identify microbiota aberrations that are associated with disease. Most of this work has been targeting the large intestine and fecal microbiota, while the small intestine microbiota may also have a profound imp...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.09.005
更新日期：2015-04-01 00:00:00
abstract：:Recent advances in the use of fluorescence spectroscopy to study protein interactions have primarily involved combinations of classic fluorescence techniques, novel probe and coupling chemistries, and advances in laser excitation and detection capabilities. For example, new coupling strategies for fluorescent probes h...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/s0958-1669(97)80156-5
更新日期：1997-02-01 00:00:00
abstract：:Soft rot pathogens cause significant losses worldwide in fruit and vegetable production during the growing season and after harvest. These pathogens engage in molecular interactions with their plant hosts via acyl-homoserine lactone (AHL) quorum sensing, pectin metabolites, phenolic acids, and auxin. The AHL-based int...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2009.01.005
更新日期：2009-04-01 00:00:00
abstract：:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the p...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00116-6
更新日期：2000-08-01 00:00:00
abstract：:In recent years a number of aglycosylated therapeutic antibodies have entered the clinic. The clinical evaluation of these antibodies has served to dispel concerns that the absence of the ubiquitous N297 glycan in the Fc of IgG might result in immunogenicity, poor in vivo stability or unfavorable pharmacokinetics. Imp...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.03.002
更新日期：2011-12-01 00:00:00
abstract：:Lignin is a principal structural component of cell walls in higher terrestrial plants. It reinforces the cell walls, facilitates water transport, and acts as a physical barrier to pathogens. Lignin is typically described as being composed of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) units that derive from th...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2019.02.018
更新日期：2019-04-01 00:00:00
abstract：:Synthetic biology combines knowledge from various disciplines including molecular biology, engineering, mathematics and physics to design and build novel proteins, genetic circuits and metabolic networks. Early efforts aimed at altering the behavior of individual elements have now evolved to focus on the construction ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2005.07.002
更新日期：2005-08-01 00:00:00
abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.10.004
更新日期：2013-06-01 00:00:00
abstract：:The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowled...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(97)80063-8
更新日期：1997-08-01 00:00:00
abstract：:The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.10.007
更新日期：2015-04-01 00:00:00
abstract：:Fibrotic diseases occur in virtually every tissue of the body and are a major cause of mortality, yet they remain largely untreatable and poorly understood on a mechanistic level. The development of anti-fibrotic agents has been hampered, in part, by the insufficient fibrosis biomimicry provided by traditional in vitr...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.02.006
更新日期：2016-08-01 00:00:00
abstract：:The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerf...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2017.06.001
更新日期：2017-12-01 00:00:00
abstract：:Plant-based foods offer a wide range of nutrients that are essential for human and animal health. Among these nutrients, iron stands out as one of the most important micronutrients. Increasing the iron content in many staple and non-staple plant foods continues to be a goal of many scientists around the world. However...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.10.001
更新日期：2017-04-01 00:00:00
abstract：:Microbial communities are essential for most ecosystem processes and interact in highly complex ways with virtually all eukaryotes. Thus, a detailed understanding of the function of such communities is a fundamental prerequisite for microbial ecologists, applied microbiologists and microbiome researchers. Using single...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.04.018
更新日期：2016-10-01 00:00:00
abstract：:Oil prices and government mandates have catalyzed rapid growth of nonfossil transportation fuels in recent years, with a large focus on ethanol from energy crops, but the food crops used as first-generation energy crops today are not optimized for this purpose. We show that the theoretical efficiency of conversion of ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/j.copbio.2008.05.001
更新日期：2008-06-01 00:00:00
