Abstract:
:Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically modulated and set in a dynamic fluctuating environment within a living cell. To build more complex circuits and to gain insight into context effects, intrinsic noise and transient performance, characterisation techniques that resolve both heterogeneity and dynamics are required. Here we review recent advances in both in vitro and in vivo microfluidic technologies that are suitable for the characterisation of synthetic circuitry, modules and parts.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Prangemeier T,Lehr FX,Schoeman RM,Koeppl Hdoi
10.1016/j.copbio.2020.02.002subject
Has Abstractpub_date
2020-06-01 00:00:00pages
167-176eissn
0958-1669issn
1879-0429pii
S0958-1669(20)30012-4journal_volume
63pub_type
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