Poxviruses as expression vectors.

abstract：:Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...

journal_title：Current opinion in biotechnology

authors： Labrou NE,Papageorgiou AC,Pavli O,Flemetakis E

更新日期：2015-04-01 00:00:00

abstract：:Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

journal_title：Current opinion in biotechnology

authors： Watanabe K

更新日期：2001-06-01 00:00:00

abstract：:Dramatic technical progress in RNA synthesis and structure determination has allowed several difficulties inherent to the preparation, handling and structural analysis of RNA to be overcome, and this has led to a wealth of information about RNA structure and its relationship with biological function. It is now fully r...

journal_title：Current opinion in biotechnology

authors： Hermann T,Westhof E

更新日期：1998-02-01 00:00:00

abstract：:The development of electrospray and matrix-assisted laser desorption mass spectrometry has provided protein chemists with tools for peptide and protein structure analysis with unprecedented sensitivity and molecular weight range. The two technologies can be viewed as competitive with respect to their molecular mass de...

journal_title：Current opinion in biotechnology

authors： Lee TD,Shively JE

更新日期：1991-02-01 00:00:00

abstract：:Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yie...

journal_title：Current opinion in biotechnology

authors： Wen F,Nair NU,Zhao H

更新日期：2009-08-01 00:00:00

abstract：:Bacterial whole-cell biosensors are very useful for toxicity measurements of various samples. Semi-specific biosensors, containing fusions of stress-regulated promoters and reporter genes, have several advantages over the traditional, general biosensors that are based on constitutively expressed reporter genes. Furthe...

journal_title：Current opinion in biotechnology

authors： Sørensen SJ,Burmølle M,Hansen LH

更新日期：2006-02-01 00:00:00

abstract：:Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....

journal_title：Current opinion in biotechnology

authors： Krumlauf R

更新日期：1991-12-01 00:00:00

abstract：:The continuing expansion of protein and genome sequence databases is an opportunity to identify novel enzymes with biotechnological applications. Whether applied to enzymology, chemical biology, systems biology, and microbiology, database mining must be 'user-friendly' so that experimentalists can devise focused strat...

journal_title：Current opinion in biotechnology

authors： Zallot R,Oberg N,Gerlt JA

更新日期：2021-01-05 00:00:00

abstract：:Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead...

journal_title：Current opinion in biotechnology

authors： Carrillo-Carrion C,Carril M,Parak WJ

更新日期：2017-08-01 00:00:00

abstract：:Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted s...

journal_title：Current opinion in biotechnology

authors： Sayler GS,Fleming JT,Nivens DE

更新日期：2001-10-01 00:00:00

abstract：:Microfluidic systems have emerged as revolutionary new platform technologies for a range of applications, from consumer products such as inkjet printer cartridges to lab-on-a-chip diagnostic systems. Recent developments have opened the door to a new set of opportunities for microfluidic systems, in the field of tissue...

journal_title：Current opinion in biotechnology

authors： Inamdar NK,Borenstein JT

更新日期：2011-10-01 00:00:00

abstract：:Heterogeneity of industrial production cultures is accepted to a certain degree; however, the underlying mechanisms are seldom perceived or included in the development of new bioprocess control strategies. Population heterogeneity and its basics, perceptible in the diverse proficiency of cells, begins with asymmetric ...

journal_title：Current opinion in biotechnology

authors： Müller S,Harms H,Bley T

更新日期：2010-02-01 00:00:00

abstract：:As the consumption of seafood and shellfish increases around the world, so is the incidence of associated outbreaks of illness. Various postharvest treatments are effective at killing seafood-associated bacteria, but most of these treatments also kill the mollusks. Because consumer preferences for raw live shellfish p...

journal_title：Current opinion in biotechnology

authors： Teplitski M,Wright AC,Lorca G

更新日期：2009-04-01 00:00:00

abstract：:Growing interest in RNAs for biomedical, agricultural, and other applications has necessitated the development of methods for producing oligoribonucleotides in milligram, or larger, quantities. Recent research on in vitro transcription has focused on optimizing solution conditions and the use of immobilized templates....

journal_title：Current opinion in biotechnology

authors： Davis RH

更新日期：1995-04-01 00:00:00

abstract：:Despite their central role in directing some of the most complex biological processes, carbohydrates--nature's other information carrying biopolymer--have been largely ignored as building blocks for synthetic hierarchical assemblies. The non-stoichiometric binding and astronomical diversity characteristic of carbohydr...

journal_title：Current opinion in biotechnology

authors： Han X,Zheng Y,Munro CJ,Ji Y,Braunschweig AB

更新日期：2015-08-01 00:00:00

abstract：:Combinatorial biocatalysis is an emerging technology in the field of drug discovery. The biocatalytic approach to combinatorial chemistry uses enzymatic, chemoenzymatic, and microbial transformations to generate libraries from lead compounds. Important recent advances in combinatorial biocatalysis include iterative de...

journal_title：Current opinion in biotechnology

authors： Altreuter DH,Clark DS

更新日期：1999-04-01 00:00:00

abstract：:The use of novel materials in the nano-scale size range for applications in devices, drugs and diagnostic agents comes with a number of new opportunities, and also serious challenges to human applications. The larger size of particulate-based agents, as compared to traditional drugs, allows for the significant advanta...

journal_title：Current opinion in biotechnology

authors： Scheinberg DA,Grimm J,Heller DA,Stater EP,Bradbury M,McDevitt MR

更新日期：2017-08-01 00:00:00

abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

journal_title：Current opinion in biotechnology

authors： Pais DA,Carrondo MJ,Alves PM,Teixeira AP

更新日期：2014-12-01 00:00:00

abstract：:Recent advances in emerging food-processing technologies, such as high hydrostatic pressure or high-intensity electric field pulses, allow targeted and sophisticated modification and preservation of foods. We are beginning to understand the mechanisms involved in pressure inactivation of bacterial spores and have been...

journal_title：Current opinion in biotechnology

authors： Knorr D

更新日期：1999-10-01 00:00:00

abstract：:Microbial cell factory performance is significantly affected by the cell morphology and membrane homeostasis. It is important to ensure that cell factories are able to regulate cell morphology and maintain membrane homeostasis. Cell morphology can be controlled by regulating the formation of elongasomes and divisomes,...

journal_title：Current opinion in biotechnology

authors： Guo L,Pang Z,Gao C,Chen X,Liu L

更新日期：2020-12-01 00:00:00

abstract：:Silk combines biological properties, such as non-toxicity and biodegradability, with physico-chemical ones, for example, mechanical strength. Base on molecular engineering, nowadays also new non-silk functions can be implemented in silk materials. Driven by rational design and ingenuity, innovative recombinant silk pr...

journal_title：Current opinion in biotechnology

authors： Saric M,Scheibel T

更新日期：2019-12-01 00:00:00

abstract：:The selection of an expression platform for recombinant biopharmaceuticals is often centered upon suitable product titers and critical quality attributes, including post-translational modifications. Although notable differences between microbial, yeast, plant, and mammalian host systems exist, recent advances have gre...

journal_title：Current opinion in biotechnology

authors： Meehl MA,Stadheim TA

更新日期：2014-12-01 00:00:00

abstract：:Vaccines against mutating pathogens such as influenza, HIV, or plasmodium are poorly protective towards new evolving strains. Rare individuals naturally mount broadly neutralizing antibodies covering most strains, but the requirements for their induction are unknown. The antibody response to vaccination has been recap...

journal_title：Current opinion in biotechnology

authors： Robert PA,Marschall AL,Meyer-Hermann M

更新日期：2018-06-01 00:00:00

abstract：:A growing body of evidence indicates that many cellular reactions within metabolic pathways are catalyzed not by free-floating 'soluble' enzymes, but via one or more membrane-associated multienzyme complexes. This type of macromolecular organization has important implications for the overall efficiency, specificity, a...

journal_title：Current opinion in biotechnology

authors： Conrado RJ,Varner JD,DeLisa MP

更新日期：2008-10-01 00:00:00

abstract：:Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

journal_title：Current opinion in biotechnology

authors： Wagner H,Heit A,Schmitz F,Bauer S

更新日期：2004-12-01 00:00:00

abstract：:In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

journal_title：Current opinion in biotechnology

authors： Gumpert J,Hoischen C

更新日期：1998-10-01 00:00:00

abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...

journal_title：Current opinion in biotechnology

authors： McGrath SP,Zhao FJ

更新日期：2003-06-01 00:00:00

abstract：:With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

journal_title：Current opinion in biotechnology

authors： Karst DJ,Steinebach F,Morbidelli M

更新日期：2018-10-01 00:00:00

abstract：:Vernalization is the process by which flowering is promoted by prolonged exposure to the cold of a typical winter. In certain plant species, the role of vernalization is to suppress the expression of genes that encode repressors of flowering. In Arabidopsis, this suppression is an epigenetic phenomenon in the sense th...

journal_title：Current opinion in biotechnology

authors： Amasino RM

更新日期：2005-04-01 00:00:00

abstract：:The increase in quality, quantity, and complexity of recombinant products heavily drives the need to predictably engineer model and complex (mammalian) cell systems. However, until recently, limited tools offered the ability to precisely manipulate their genomes, thus impeding the full potential of rational cell line ...

journal_title：Current opinion in biotechnology

authors： Cheng JK,Alper HS

更新日期：2014-12-01 00:00:00