Abstract：
:Various strategies have been developed to exploit plants as bioreactors for the production of pharmaceutical antibodies, to engineer antibody-mediated pathogen resistance or to alter the plant phenotype by immunomodulation. Recent research developments focus on the fine-tuning of expression systems and the detailed characterisation of recombinant products, including the implications of plant-specific glycosylation. Meanwhile, the first of these plant-derived antibody products has successfully completed early phase clinical trials.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Stoger E,Sack M,Fischer R,Christou Pdoi
10.1016/s0958-1669(02)00303-8subject
Has Abstractpub_date
2002-04-01 00:00:00pages
161-6issue
2eissn
0958-1669issn
1879-0429pii
S0958166902003038journal_volume
13pub_type
杂志文章,评审abstract：:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2017.02.011
更新日期：2017-08-01 00:00:00
abstract：:Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.12.024
更新日期：2015-04-01 00:00:00
abstract：:Characterization of the various microbial populations present in exoelectrogenic biofilms provides insight into the processes required to convert complex organic matter in wastewater streams into electrical current in bioelectrochemical systems (BESs). Analysis of the community profiles of exoelectrogenic microbial co...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/j.copbio.2011.03.003
更新日期：2011-06-01 00:00:00
abstract：:Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/s0958-1669(97)80153-x
更新日期：1997-02-01 00:00:00
abstract：:Both prokaryotic and eukaryotic cells respond to the accumulation of unfolded proteins by increasing the transcription of genes encoding molecular chaperones and other stress-responsive proteins. Different sets of genes are activated when particular cellular compartments are burdened with unfolded proteins. Cells thus...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(94)90071-x
更新日期：1994-10-01 00:00:00
abstract：:Growing interest in RNAs for biomedical, agricultural, and other applications has necessitated the development of methods for producing oligoribonucleotides in milligram, or larger, quantities. Recent research on in vitro transcription has focused on optimizing solution conditions and the use of immobilized templates....
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(95)80035-2
更新日期：1995-04-01 00:00:00
abstract：:Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in severa...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00166-x
更新日期：2001-02-01 00:00:00
abstract：:Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(02)00380-4
更新日期：2002-12-01 00:00:00
abstract：:Novel biotechnological approaches such as Metabolic Engineering (ME) and New Plant Breeding Techniques (NPBTs) are currently being developed to produce biological compounds for food and non-food products. NPBTs span a range of methods for in vivo production in crops, some of which are classified as GMOs while others a...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2018.08.012
更新日期：2019-04-01 00:00:00
abstract：:Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connec...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.03.018
更新日期：2016-10-01 00:00:00
abstract：:Nutraceuticals are important natural bioactive compounds that confer health-promoting and medical benefits to humans. Globally growing demands for value-added nutraceuticals for prevention and treatment of human diseases have rendered nutraceuticals a multi-billion dollar market. However, supply limitations and extrac...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2015.11.003
更新日期：2016-02-01 00:00:00
abstract：:The recent adoption of whole plasmid genome sequencing as a routine analytical technique has provided the basis for cataloging the historical events through which plasmids are assembled from the available families of modular plasmid components. Horizontal gene transfer mediated by plasmids plays an important role in t...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2005.04.002
更新日期：2005-06-01 00:00:00
abstract：:With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.06.012
更新日期：2011-12-01 00:00:00
abstract：:In the past year, significant progress in the field of gene transfer has been made possible by refinement of the technique of particle bombardment. The process has been utilized for the study of gene expression in plastids and mitochondria, the production of transgenic crop plants and gene transfer into live animals. ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(93)90081-7
更新日期：1993-10-01 00:00:00
abstract：:Recent advances in the use of fluorescence spectroscopy to study protein interactions have primarily involved combinations of classic fluorescence techniques, novel probe and coupling chemistries, and advances in laser excitation and detection capabilities. For example, new coupling strategies for fluorescent probes h...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/s0958-1669(97)80156-5
更新日期：1997-02-01 00:00:00
abstract：:Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00077-x
更新日期：2000-04-01 00:00:00
abstract：:Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90167-h
更新日期：1992-08-01 00:00:00
abstract：:Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2019.05.010
更新日期：2019-12-01 00:00:00
abstract：:It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90072-q
更新日期：1992-10-01 00:00:00
abstract：:RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90117-2
更新日期：1992-02-01 00:00:00
abstract：:Random peptide libraries displayed on phage are used as a source of peptides for epitope mapping, for the identification of critical amino acids responsible for protein-protein interactions and as leads for the discovery of new therapeutics. Efficient and simple procedures have been devised to select peptides binding ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80072-3
更新日期：1996-12-01 00:00:00
abstract：:Fermented foods and beverages are an integral part of the human diet globally. Understanding the microbial interactions within these fermenting ecosystems is required to deliver safe products with desirable consumer properties, and moreover, maintenance of these traditions. Effective tools are required for documentati...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.08.004
更新日期：2013-04-01 00:00:00
abstract：:Much progress has been made in the development of techniques for constructing dense grids either of ligands, such as peptides and oligonucleotides, or of cloned nucleic acids. Such arrays are finding practical applications in the analysis of sequence variation and gene expression. Methods for carrying out large number...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80100-5
更新日期：1996-02-01 00:00:00
abstract：:The intestinal microbiota plays a profound role in human health and extensive research has been dedicated to identify microbiota aberrations that are associated with disease. Most of this work has been targeting the large intestine and fecal microbiota, while the small intestine microbiota may also have a profound imp...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.09.005
更新日期：2015-04-01 00:00:00
abstract：:Progress in biosensors has mainly been made by the improvement of the biological components and the implementation of microsystem technologies. Enzymes are still the most appropriate recognition elements because they combine high chemical specificity and inherent biocatalytic signal amplification. A breakthrough has b...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00169-5
更新日期：2001-02-01 00:00:00
abstract：:Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.10.011
更新日期：2017-08-01 00:00:00
abstract：:The demand for validated analytical methods for botanicals has grown in response to the increasing consumer market for botanical supplements. Government initiatives to increase the availability of validated analytical methods and botanical reference material have led to the publication of numerous validation studies i...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2013.10.003
更新日期：2014-02-01 00:00:00
abstract：:Adaptive laboratory evolution (ALE) strategies allow for the metabolic engineering of microorganisms by combining genetic variation with the selection of beneficial mutations in an unbiased fashion. These ALE strategies have been proven highly effective in the optimization of production strains. In contrast to rationa...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.03.007
更新日期：2011-08-01 00:00:00
abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.10.009
更新日期：2012-04-01 00:00:00
abstract：:Microfluidic systems have emerged as revolutionary new platform technologies for a range of applications, from consumer products such as inkjet printer cartridges to lab-on-a-chip diagnostic systems. Recent developments have opened the door to a new set of opportunities for microfluidic systems, in the field of tissue...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.05.512
更新日期：2011-10-01 00:00:00
