Static and dynamic light scattering of biological macromolecules: what can we learn?

abstract：:Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...

journal_title：Current opinion in biotechnology

authors： Lee PS,Lee KH

更新日期：2000-04-01 00:00:00

abstract：:Recombinant DNA technology has expanded the potential of the targeted screening approach in drug discovery. The use of cloned and expressed receptors as targets for the evaluation of novel compounds can significantly simplify the structure affinity/activity process. However, the patenting of cloned receptors, as well ...

journal_title：Current opinion in biotechnology

authors： Williams M

更新日期：1993-02-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:Characterization of the various microbial populations present in exoelectrogenic biofilms provides insight into the processes required to convert complex organic matter in wastewater streams into electrical current in bioelectrochemical systems (BESs). Analysis of the community profiles of exoelectrogenic microbial co...

journal_title：Current opinion in biotechnology

authors： Kiely PD,Regan JM,Logan BE

更新日期：2011-06-01 00:00:00

abstract：:Various strategies have been developed to exploit plants as bioreactors for the production of pharmaceutical antibodies, to engineer antibody-mediated pathogen resistance or to alter the plant phenotype by immunomodulation. Recent research developments focus on the fine-tuning of expression systems and the detailed ch...

journal_title：Current opinion in biotechnology

authors： Stoger E,Sack M,Fischer R,Christou P

更新日期：2002-04-01 00:00:00

abstract：:Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...

journal_title：Current opinion in biotechnology

authors： Sligar SG,Salemme FR

更新日期：1992-08-01 00:00:00

abstract：:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

journal_title：Current opinion in biotechnology

authors： Rashtchian A

更新日期：1995-02-01 00:00:00

abstract：:The fundamental role of glycoconjugates in many biological processes is now well appreciated and has intensified the development of innovative and improved synthetic strategies. All areas of synthetic methodology have seen major advances and many complex, highly branched carbohydrates and glycoproteins have been prepa...

journal_title：Current opinion in biotechnology

authors： Hölemann A,Seeberger PH

更新日期：2004-12-01 00:00:00

abstract：:The demand for validated analytical methods for botanicals has grown in response to the increasing consumer market for botanical supplements. Government initiatives to increase the availability of validated analytical methods and botanical reference material have led to the publication of numerous validation studies i...

journal_title：Current opinion in biotechnology

authors： Brown PN,Lister P

更新日期：2014-02-01 00:00:00

abstract：:Understanding the hierarchical structure of proteins at their fundamental length scales is essential to get insights into their functions and roles in fundamental biological processes. Near-field scanning optical microscopy (NSOM), which overcomes the diffraction limits of conventional optics, provides a powerful anal...

journal_title：Current opinion in biotechnology

authors： Yong YC,Wang YZ,Zhong JJ

更新日期：2018-12-01 00:00:00

abstract：:Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes. The number of molecules identified and characterized, however, is in clear opposition to the number of molecules that are ente...

journal_title：Current opinion in biotechnology

authors： Maurer KH

更新日期：2004-08-01 00:00:00

abstract：:Advances in metabolic engineering have given rise to the biological production of novel fuels and chemicals, but yields are often low without significant optimization. One generalizable solution is to create a specialized organelle for the sequestration of engineered metabolic pathways. Bacterial microcompartments are...

journal_title：Current opinion in biotechnology

authors： Kim EY,Tullman-Ercek D

更新日期：2013-08-01 00:00:00

abstract：:NMR methods in drug discovery have traditionally been used to obtain structural information for drug targets or target-ligand complexes. Recently, it has been shown that NMR may be used as an alternative approach for identification of ligands that bind to protein drug targets, shifting the emphasis of many NMR laborat...

journal_title：Current opinion in biotechnology

authors： Moore JM

更新日期：1999-02-01 00:00:00

abstract：:The metabolome, the collection of small molecules associated with an organism, is a growing subject of inquiry, with the data utilized for data-intensive systems biology, disease diagnostics, biomarker discovery, and the broader characterization of small molecules in mixtures. Owing to their close proximity to the fun...

journal_title：Current opinion in biotechnology

authors： Eghbalnia HR,Romero PR,Westler WM,Baskaran K,Ulrich EL,Markley JL

更新日期：2017-02-01 00:00:00

abstract：:Progress in the field of protein nuclear magnetic resonance spectroscopy during the past year has included the elucidation of a number of new structures. In addition, several critical developments in the experimental methodology have opened up the potential for applying the nuclear magnetic resonance-based approach to...

journal_title：Current opinion in biotechnology

authors： Chazin WJ

更新日期：1991-08-01 00:00:00

abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...

journal_title：Current opinion in biotechnology

authors： McGrath SP,Zhao FJ

更新日期：2003-06-01 00:00:00

abstract：:Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated biochemical reactions by in vitro assembling a number of enzymes or their complexes and coenzymes. Assembly of numerous enzymes without cellular membrane, gene regulation, or undesired pathway can circumvent some of the obstacle...

journal_title：Current opinion in biotechnology

authors： Zhang YH,Sun J,Zhong JJ

更新日期：2010-10-01 00:00:00

abstract：:In recent years, our understanding about bacterial persistence has significantly advanced: we comprehend the persister phenotype better, more triggers for persistence entry have been found, and more insights in the involvement and role of toxin-antitoxin systems and other molecular mechanisms have been unravelled. In ...

journal_title：Current opinion in biotechnology

authors： Radzikowski JL,Schramke H,Heinemann M

更新日期：2017-08-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:Tremendous advances in biocatalytic approaches to oligosaccharide synthesis have taken place in the past two years. The use of isolated enzymes, both glycosyltransferases and glycosidases, or engineered whole cells allows the preparation of natural oligosaccharides and analogs required for glycobiology research. ...

journal_title：Current opinion in biotechnology

authors： Palcic MM

更新日期：1999-12-01 00:00:00

abstract：:Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels. In the past decade, novel hydrocar...

journal_title：Current opinion in biotechnology

authors： Liu K,Li S

更新日期：2020-04-01 00:00:00

abstract：:Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites b...

journal_title：Current opinion in biotechnology

authors： Ohta D,Kanaya S,Suzuki H

更新日期：2010-02-01 00:00:00

abstract：:Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

journal_title：Current opinion in biotechnology

authors： Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

更新日期：2019-04-01 00:00:00

abstract：:Bioremediation has historically been approached as a 'black box' in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at t...

journal_title：Current opinion in biotechnology

authors： Chakraborty R,Wu CH,Hazen TC

更新日期：2012-06-01 00:00:00

abstract：:Patient-derived xenografts (PDXs) are obtained by transplanting fragments of a patient's tumour into immunodeficient mice. Growth and propagation of PDXs allows correlating therapeutic response in vivo with extensive, multi-dimensional molecular annotation, leading to identification of predictive biomarkers. PDXs are ...

journal_title：Current opinion in biotechnology

authors： Invrea F,Rovito R,Torchiaro E,Petti C,Isella C,Medico E

更新日期：2020-06-01 00:00:00

abstract：:Heterotrophic bacteria are an integral part of any environmental biotechnology process (EBP). Therefore, factors controlling bacterial abundance, activity, and community composition are central to the understanding of such processes. Among these factors, top-down control by bacteriophage predation has so far received ...

journal_title：Current opinion in biotechnology

authors： Shapiro OH,Kushmaro A

更新日期：2011-06-01 00:00:00

abstract：:Transportation fuels will gradually shift from oil based fuels towards alternative fuel resources like biofuels. Current bioethanol and biodiesel can, however, not cover the increasing demand for biofuels and there is therefore a need for advanced biofuels with superior fuel properties. Novel cell factories will provi...

journal_title：Current opinion in biotechnology

authors： de Jong B,Siewers V,Nielsen J

更新日期：2012-08-01 00:00:00

abstract：:Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...

journal_title：Current opinion in biotechnology

authors： Park D,Swayambhu G,Pfeifer BA

更新日期：2020-12-01 00:00:00

abstract：:Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...

journal_title：Current opinion in biotechnology

authors： Newton RJ,McClary JS

更新日期：2019-06-01 00:00:00

abstract：:Fc fusion proteins are molecules in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. Fc fusion proteins have some antibody-like properties such as long serum half-life and easy expression and purification, making them ...

journal_title：Current opinion in biotechnology

authors： Huang C

更新日期：2009-12-01 00:00:00