Abstract:
:Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated biochemical reactions by in vitro assembling a number of enzymes or their complexes and coenzymes. Assembly of numerous enzymes without cellular membrane, gene regulation, or undesired pathway can circumvent some of the obstacles to modifying living microorganisms. Several synthetic pathways for the production of liquid biofuels--alcohols and hydrocarbon precursors (polyols) as well as gaseous biofuel--hydrogen have been presented. The present constraints to SyPaB include the lack of stable enzymes as Lego-like building blocks, the different optimal reaction conditions for individual enzyme, and the use of costly labile coenzymes. It is expected that high-yield SyPaB will be an important platform for producing low-cost biofuels and biochemicals.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Zhang YH,Sun J,Zhong JJdoi
10.1016/j.copbio.2010.05.005subject
Has Abstractpub_date
2010-10-01 00:00:00pages
663-9issue
5eissn
0958-1669issn
1879-0429pii
S0958-1669(10)00101-1journal_volume
21pub_type
杂志文章,评审abstract::Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.08.003
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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abstract::Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites b...
journal_title:Current opinion in biotechnology
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abstract::Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....
journal_title:Current opinion in biotechnology
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abstract::Antisense technology is finding increasing application not only in clinical development, but also for cellular engineering. Several types of antisense methods (e.g. antisense oligonucleotides, antisense RNA and small interfering RNA) can be used to inhibit the expression of a target gene. These antisense methods are b...
journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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abstract::Growing interest in RNAs for biomedical, agricultural, and other applications has necessitated the development of methods for producing oligoribonucleotides in milligram, or larger, quantities. Recent research on in vitro transcription has focused on optimizing solution conditions and the use of immobilized templates....
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2016.10.011
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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更新日期:2017-04-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2005.12.007
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(00)00116-6
更新日期:2000-08-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2003.10.014
更新日期:2003-12-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0958-1669(94)90061-2
更新日期:1994-10-01 00:00:00
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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