Total internal reflection fluorescence microscopy: technical innovations and novel applications.

Abstract:

:Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel applications have concentrated on single-molecule detection (e.g. of cellular receptors), imaging of exocytosis or endocytosis, measurements of adhesion foci of microtubules, and studies of the localization, activity and structural arrangement of specific ion channels. In addition to conventional fluorescent dyes, genetically engineered fluorescent proteins are increasingly being used to measure molecular conformations or intermolecular distances by fluorescence resonance energy transfer.

journal_name

Curr Opin Biotechnol

authors

Schneckenburger H

doi

10.1016/j.copbio.2004.12.004

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

13-8

issue

1

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(04)00169-7

journal_volume

16

pub_type

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