Total internal reflection fluorescence microscopy: technical innovations and novel applications.


:Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel applications have concentrated on single-molecule detection (e.g. of cellular receptors), imaging of exocytosis or endocytosis, measurements of adhesion foci of microtubules, and studies of the localization, activity and structural arrangement of specific ion channels. In addition to conventional fluorescent dyes, genetically engineered fluorescent proteins are increasingly being used to measure molecular conformations or intermolecular distances by fluorescence resonance energy transfer.


Curr Opin Biotechnol


Schneckenburger H




Has Abstract


2005-02-01 00:00:00














  • Electron-capture dissociation tandem mass spectrometry.

    abstract::Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Zubarev RA

    更新日期:2004-02-01 00:00:00

  • 'Hybrid' processing strategies for expanding and improving the synthesis of renewable bioproducts.

    abstract::The versatile functionality of microbial biocatalysts offers a promising solution to the growing need to replace conventional, petroleum-derived fuels, chemicals, and materials with sustainable alternatives from renewable biomass. Whereas metabolic pathway engineering and strain optimization have greatly expanded the ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Thompson B,Moon TS,Nielsen DR

    更新日期:2014-12-01 00:00:00

  • Bioremedial potential of microbial mechanisms of metal mobilization and immobilization.

    abstract::Microorganisms play important roles in the environmental fate of toxic metals and radionuclides with a multiplicity of mechanisms effecting transformations between soluble and insoluble forms. These mechanisms are integral components of natural biogeochemical cycles and are of potential for both in situ and ex situ bi...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Gadd GM

    更新日期:2000-06-01 00:00:00

  • Microbial biocatalyst developments to upgrade fossil fuels.

    abstract::Steady increases in the average sulfur content of petroleum and stricter environmental regulations concerning the sulfur content have promoted studies of bioprocessing to upgrade fossil fuels. Bioprocesses can potentially provide a solution to the need for improved and expanded fuel upgrading worldwide, because biopro...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kilbane JJ 2nd

    更新日期:2006-06-01 00:00:00

  • Current initiatives for the validation of analytical methods for botanicals.

    abstract::The demand for validated analytical methods for botanicals has grown in response to the increasing consumer market for botanical supplements. Government initiatives to increase the availability of validated analytical methods and botanical reference material have led to the publication of numerous validation studies i...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Brown PN,Lister P

    更新日期:2014-02-01 00:00:00

  • The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology.

    abstract::Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kildegaard HF,Baycin-Hizal D,Lewis NE,Betenbaugh MJ

    更新日期:2013-12-01 00:00:00

  • Synergistic use of adult and embryonic stem cells to study human hematopoiesis.

    abstract::Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Martin CH,Kaufman DS

    更新日期:2005-10-01 00:00:00

  • Advances in the analysis of single mitochondria.

    abstract::Mitochondria are both morphologically and functionally diverse, and this variety is thought to have important biological ramifications. The development of methods to probe the properties of individual mitochondria is therefore of utmost importance. Recent advances have been made using in situ microscopy techniques and...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Fuller KM,Arriaga EA

    更新日期:2003-02-01 00:00:00

  • Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production.

    abstract::The biosynthesis of the solvents 1-butanol and acetone is restricted to species of the genus Clostridium, a diverse group of Gram-positive, endospore forming anaerobes comprising toxin-producing strains as well as terrestrial non-pathogenic species of biotechnological impact. Among solventogenic clostridia, Clostridiu...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lütke-Eversloh T,Bahl H

    更新日期:2011-10-01 00:00:00

  • Gut metabotypes govern health effects of dietary polyphenols.

    abstract::Polyphenols are thought to be responsible for some of the health effects conferred by a diet rich in fruit and vegetables. Both the formation of bioactive polyphenol-derived metabolites and the modulation of colonic microbiota contribute to these health benefits. Therefore, one cannot infer biological responses from d...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bolca S,Van de Wiele T,Possemiers S

    更新日期:2013-04-01 00:00:00

  • Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project.

    abstract::The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet ke...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Parinov S,Sundaresan V

    更新日期:2000-04-01 00:00:00

  • Heterologous biosynthesis as a platform for producing new generation natural products.

    abstract::Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Park D,Swayambhu G,Pfeifer BA

    更新日期:2020-12-01 00:00:00

  • Novel approaches in food-processing technology: new technologies for preserving foods and modifying function.

    abstract::Recent advances in emerging food-processing technologies, such as high hydrostatic pressure or high-intensity electric field pulses, allow targeted and sophisticated modification and preservation of foods. We are beginning to understand the mechanisms involved in pressure inactivation of bacterial spores and have been...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Knorr D

    更新日期:1999-10-01 00:00:00

  • Genomic analysis.

    abstract::Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lee PS,Lee KH

    更新日期:2000-04-01 00:00:00

  • Novel methods for cloning and engineering genes using the polymerase chain reaction.

    abstract::Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rashtchian A

    更新日期:1995-02-01 00:00:00

  • Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli.

    abstract::Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage. Given this situation, one would predict translational problems with an abundant...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kane JF

    更新日期:1995-10-01 00:00:00

  • Advances in quantitative proteomics via stable isotope tagging and mass spectrometry.

    abstract::The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Tao WA,Aebersold R

    更新日期:2003-02-01 00:00:00

  • The interplay between microevolution and community structure in microbial populations.

    abstract::The structure of microbial communities is key to their functionality. However, this structure is likely to be influenced by adaptive genetic change in members of the community, which can occur over a matter of days. Changes in community structure can in turn influence the evolutionary trajectories of species within th...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: O'Brien S,Hodgson DJ,Buckling A

    更新日期:2013-08-01 00:00:00

  • Progress in computational protein design.

    abstract::Current progress in computational structure-based protein design is reviewed in the areas of methodology and applications. Foundational advances include new potential functions, more efficient ways of computing energetics, flexible treatments of solvent, and useful energy function approximations, as well as ensemble-b...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lippow SM,Tidor B

    更新日期:2007-08-01 00:00:00

  • Biological function made crystal clear - annotation of hypothetical proteins via structural genomics.

    abstract::Many of the gene products of completely sequenced organisms are 'hypothetical' - they cannot be related to any previously characterized proteins - and so are of completely unknown function. Structural studies provide one means of obtaining functional information in these cases. A 'structural genomics' project has been...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Eisenstein E,Gilliland GL,Herzberg O,Moult J,Orban J,Poljak RJ,Banerjei L,Richardson D,Howard AJ

    更新日期:2000-02-01 00:00:00

  • Plant GSTome: structure and functional role in xenome network and plant stress response.

    abstract::Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Labrou NE,Papageorgiou AC,Pavli O,Flemetakis E

    更新日期:2015-04-01 00:00:00

  • Population-level approaches reveal novel aspects of lignin biosynthesis, content, composition and structure.

    abstract::Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

    更新日期:2019-04-01 00:00:00

  • Industrial microbial enzymes: their discovery by screening and use in large-scale production of useful chemicals in Japan.

    abstract::The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Ogawa J,Shimizu S

    更新日期:2002-08-01 00:00:00

  • Membrane platforms for biological nanopore sensing and sequencing.

    abstract::In the past two decades, biological nanopores have been developed and explored for use in sensing applications as a result of their exquisite sensitivity and easily engineered, reproducible, and economically manufactured structures. Nanopore sensing has been shown to differentiate between highly similar analytes, meas...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Schmidt J

    更新日期:2016-06-01 00:00:00

  • Cranberry components for the therapy of infectious disease.

    abstract::Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

    更新日期:2012-04-01 00:00:00

  • The evolution of IncP catabolic plasmids.

    abstract::The recent adoption of whole plasmid genome sequencing as a routine analytical technique has provided the basis for cataloging the historical events through which plasmids are assembled from the available families of modular plasmid components. Horizontal gene transfer mediated by plasmids plays an important role in t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Dennis JJ

    更新日期:2005-06-01 00:00:00

  • Visualization of data.

    abstract::Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Meyer RD,Cook D

    更新日期:2000-02-01 00:00:00

  • APOE is a major susceptibility gene for Alzheimer's disease.

    abstract::The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Roses AD,Saunders AM

    更新日期:1994-12-01 00:00:00

  • Flux analysis in plant metabolic networks: increasing throughput and coverage.

    abstract::Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Junker BH

    更新日期:2014-04-01 00:00:00

  • Peptide ligation and semisynthesis.

    abstract::Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destru...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wallace CJ

    更新日期:1995-08-01 00:00:00