Abstract:
:Escherichia coli is still a very popular host for the production of recombinant proteins at an analytical or industrial scale. Secretion of the proteins into the culture medium or display at the cell surface would be preferred in many applications but is hampered by the complex two-layered cell envelope. The autotransporter pathway is used by E. coli to secrete virulence factors via a relatively simple but efficient and specific mechanism. Here we discuss recent progress in the structural and mechanistic analysis of this pathway and the implications for future development of a versatile platform for secretion and display of heterologous proteins.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Jong WS,Saurí A,Luirink Jdoi
10.1016/j.copbio.2010.07.009subject
Has Abstractpub_date
2010-10-01 00:00:00pages
646-52issue
5eissn
0958-1669issn
1879-0429pii
S0958-1669(10)00128-Xjournal_volume
21pub_type
杂志文章,评审abstract::Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...
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journal_title:Current opinion in biotechnology
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