Engineered minichromosomes in plants.

abstract：:The fundamental role of glycoconjugates in many biological processes is now well appreciated and has intensified the development of innovative and improved synthetic strategies. All areas of synthetic methodology have seen major advances and many complex, highly branched carbohydrates and glycoproteins have been prepa...

journal_title：Current opinion in biotechnology

authors： Hölemann A,Seeberger PH

更新日期：2004-12-01 00:00:00

abstract：:Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...

journal_title：Current opinion in biotechnology

authors： Martin CH,Kaufman DS

更新日期：2005-10-01 00:00:00

abstract：:The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

journal_title：Current opinion in biotechnology

authors： Tao WA,Aebersold R

更新日期：2003-02-01 00:00:00

abstract：:To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...

journal_title：Current opinion in biotechnology

authors： Azerad R,Buisson D

更新日期：2000-12-01 00:00:00

abstract：:An intense interest has grown in the unique interactions of nucleic acids with metal ions, which lead to the formation of metal-base pairs and the generation of fluorescent nanomaterials. In this review, different types of metal-base pairs, especially those formed from naturally occurring nucleosides, are described wi...

journal_title：Current opinion in biotechnology

authors： Park KS,Park HG

更新日期：2014-08-01 00:00:00

abstract：:NMR methods in drug discovery have traditionally been used to obtain structural information for drug targets or target-ligand complexes. Recently, it has been shown that NMR may be used as an alternative approach for identification of ligands that bind to protein drug targets, shifting the emphasis of many NMR laborat...

journal_title：Current opinion in biotechnology

authors： Moore JM

更新日期：1999-02-01 00:00:00

abstract：:The release of sub-picomole levels of N-linked oligosaccharides directly from 1-5 micrograms of protein in a band on an SDS PAGE gel, coupled with recent advances in mass spectrometry and HPLC, opens the way for the analysis of biologically important glycoproteins that are difficult to purify or are available only in ...

journal_title：Current opinion in biotechnology

authors： Rudd PM,Dwek RA

更新日期：1997-08-01 00:00:00

abstract：:Fatty acid oxidation pathways are attractive for metabolic engineering purposes due to their cyclic nature as well as their reactions that allow for the selective functionalization of alkyl chains. These characteristics allow for the production of various chemicals, such as alcohols, alkanes, ketones and hydroxyacids,...

journal_title：Current opinion in biotechnology

authors： Kim S,Cheong S,Chou A,Gonzalez R

更新日期：2016-12-01 00:00:00

abstract：:Plants synthesize a myriad of secondary metabolites (SMs) that are derived from central or primary metabolism. While these so-called natural products have been targets for plant metabolic engineering attempts for many years, the immense value of manipulating the interface between committed steps in secondary metabolis...

journal_title：Current opinion in biotechnology

authors： Aharoni A,Galili G

更新日期：2011-04-01 00:00:00

abstract：:Plant biotechnology has been around since the advent of humankind, resulting in tremendous improvements in plant cultivation through crop domestication, breeding and selection. The emergence of transgenic approaches involving the introduction of defined DNA sequences into plants by humans has rapidly changed the surfa...

journal_title：Current opinion in biotechnology

authors： Francis D,Finer JJ,Grotewold E

更新日期：2017-04-01 00:00:00

abstract：:Poxviruses are widely used for the cytoplasmic expression of recombinant genes in mammalian cells. Recent improvements allow high expression and simplify the integration of multiple foreign genes. Vaccinia virus mutants and other poxviruses that undergo abortive infection in mammalian cells are receiving special atten...

journal_title：Current opinion in biotechnology

authors： Carroll MW,Moss B

更新日期：1997-10-01 00:00:00

abstract：:With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

journal_title：Current opinion in biotechnology

authors： Karst DJ,Steinebach F,Morbidelli M

更新日期：2018-10-01 00:00:00

abstract：:Saccharomyces cerevisiae has decisive advantages in industrial processes due to its tolerance to alcohols and fermentation conditions. Butanol isomers are considered as suitable fuel substitutes and valuable biomass-derived chemical building blocks. Whereas high production was achieved with bacterial systems, metaboli...

journal_title：Current opinion in biotechnology

authors： Generoso WC,Schadeweg V,Oreb M,Boles E

更新日期：2015-06-01 00:00:00

abstract：:Nutraceuticals are important natural bioactive compounds that confer health-promoting and medical benefits to humans. Globally growing demands for value-added nutraceuticals for prevention and treatment of human diseases have rendered nutraceuticals a multi-billion dollar market. However, supply limitations and extrac...

journal_title：Current opinion in biotechnology

authors： Wang J,Guleria S,Koffas MA,Yan Y

更新日期：2016-02-01 00:00:00

abstract：:Wound healing can no longer be considered a generic term: it is the summation of a complex series of processes beginning with coagulation and ending in remodelling. This review, therefore, summarizes the past year's advances in growth factor research according to the roles of the individual growth factors in the speci...

journal_title：Current opinion in biotechnology

authors： Robson MC

更新日期：1991-12-01 00:00:00

abstract：:The impact of directed evolution and site-specific mutagenesis on the industrial utility of enzymatic catalysis through the modification of enzyme structure and function is clearly an important area of research in bioprocess engineering. High-throughput screening for novel or improved enzyme activities, both by more e...

journal_title：Current opinion in biotechnology

authors： Panke S,Wubbolts MG

更新日期：2002-04-01 00:00:00

abstract：:Self-assembled peptide systems have been widely studied in the context of gaining understanding of the rules that govern biomolecular processes and increasingly as new bio-inspired nanomaterials. Such materials may be designed to be highly dynamic, displaying adaptive and self-healing properties. This review focuses o...

journal_title：Current opinion in biotechnology

authors： Sadownik JW,Ulijn RV

更新日期：2010-08-01 00:00:00

abstract：:Biotechnology applications require engineering complex multi-genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole bio...

journal_title：Current opinion in biotechnology

authors： Bassalo MC,Liu R,Gill RT

更新日期：2016-06-01 00:00:00

abstract：:Adverse environmental conditions pose serious limitations to agricultural production. Classical biotechnological approaches towards increasing abiotic stress tolerance focus on boosting plant endogenous defence mechanisms. However, overexpression of regulatory elements or effectors is usually accompanied by growth han...

journal_title：Current opinion in biotechnology

authors： Cabello JV,Lodeyro AF,Zurbriggen MD

更新日期：2014-04-01 00:00:00

abstract：:Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

journal_title：Current opinion in biotechnology

authors： Friedrich MW

更新日期：2006-02-01 00:00:00

abstract：:The use of microbial consortia for bioprocessing has been limited by our ability to reliably control community composition and function simultaneously. Recent advances in synthetic biology have enabled population-level coordination and control of ecosystem stability and dynamics. Further, new experimental and computat...

journal_title：Current opinion in biotechnology

authors： Shong J,Jimenez Diaz MR,Collins CH

更新日期：2012-10-01 00:00:00

abstract：:The organization of biochemical networks that make up the living cell can be defined by studying the dynamics of protein-protein interactions. To this end, experimental strategies based on protein fragment complementation assays (PCAs) have been used to map biochemical networks and to identify novel components of thes...

journal_title：Current opinion in biotechnology

authors： Michnick SW

更新日期：2003-12-01 00:00:00

abstract：:Intraportal islet transplantation has emerged as a promising treatment for type 1 diabetes mellitus (T1DM). Nevertheless, long-term efficacy has been limited to a marginal number of patients. Outcomes have been restricted, in part, by challenges associated with the transplant site, poor vascularization, and disruption...

journal_title：Current opinion in biotechnology

authors： Coronel MM,Stabler CL

更新日期：2013-10-01 00:00:00

abstract：:Scaffolds are used in tissue engineering as a matrix for the seeding and attachment of human cells. The creation of porosity in three-dimensional (3D) structures of scaffolds plays a critical role in cell proliferation, migration, and differentiation into the specific tissue while secreting extracellular matrix compon...

journal_title：Current opinion in biotechnology

authors： Dehghani F,Annabi N

更新日期：2011-10-01 00:00:00

abstract：:Marine microbes have received growing attention as sources of bioactive metabolites and offer a unique opportunity to both increase the number of marine natural products in clinical trials as well as expedite their development. This review focuses specifically on those molecules currently in the clinical pipeline that...

journal_title：Current opinion in biotechnology

authors： Waters AL,Hill RT,Place AR,Hamann MT

更新日期：2010-12-01 00:00:00

abstract：:Progress in biosensors has mainly been made by the improvement of the biological components and the implementation of microsystem technologies. Enzymes are still the most appropriate recognition elements because they combine high chemical specificity and inherent biocatalytic signal amplification. A breakthrough has b...

journal_title：Current opinion in biotechnology

authors： Scheller FW,Wollenberger U,Warsinke A,Lisdat F

更新日期：2001-02-01 00:00:00

abstract：:As the consumption of seafood and shellfish increases around the world, so is the incidence of associated outbreaks of illness. Various postharvest treatments are effective at killing seafood-associated bacteria, but most of these treatments also kill the mollusks. Because consumer preferences for raw live shellfish p...

journal_title：Current opinion in biotechnology

authors： Teplitski M,Wright AC,Lorca G

更新日期：2009-04-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...

journal_title：Current opinion in biotechnology

authors： DelRosso NV,Derr ND

更新日期：2017-08-01 00:00:00

abstract：:Life depends on the interaction of proteins. The availability of the complete human genome sequence has highlighted the need for a tool to analyse protein interactions and several databases have been compiled for this purpose. These databases document, categorize, and analyze interacting proteins and the cellular func...

journal_title：Current opinion in biotechnology

authors： Xenarios I,Eisenberg D

更新日期：2001-08-01 00:00:00