Abstract：
:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable alternative to dredging for treatment of PCB impacted sites. Being hydrophobic, PCBs partition into organic material and accumulate in anoxic environments well poised to support anaerobic dechlorination of highly chlorinated congeners; products of which are susceptible to complete aerobic degradation. Laboratory research over the past 25 years is now leading to new microbial technologies that could soon be tested for treatment of PCB impacted sediments in the field.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Sowers KR,May HDdoi
10.1016/j.copbio.2012.10.004subject
Has Abstractpub_date
2013-06-01 00:00:00pages
482-8issue
3eissn
0958-1669issn
1879-0429pii
S0958-1669(12)00159-0journal_volume
24pub_type
杂志文章,评审abstract：:The intestinal microbiota plays a profound role in human health and extensive research has been dedicated to identify microbiota aberrations that are associated with disease. Most of this work has been targeting the large intestine and fecal microbiota, while the small intestine microbiota may also have a profound imp...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.09.005
更新日期：2015-04-01 00:00:00
abstract：:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2015.02.006
更新日期：2015-06-01 00:00:00
abstract：:Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2006.12.001
更新日期：2007-04-01 00:00:00
abstract：:The phytodegradation of organic compounds can take place inside the plant or within the rhizosphere of the plant. Many different compounds and classes of compounds can be removed from the environment by this method, including solvents in groundwater, petroleum and aromatic compounds in soils, and volatile compounds in...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2004.04.006
更新日期：2004-06-01 00:00:00
abstract：:Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are bei...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2015.05.003
更新日期：2015-12-01 00:00:00
abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80040-1
更新日期：1996-06-01 00:00:00
abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2008.02.016
更新日期：2008-04-01 00:00:00
abstract：:Bacterial cell wall hydrolases (BCWHs) display a remarkable structural and functional diversity that offers perspectives for novel food applications, reaching beyond those of the archetype BCWH and established biopreservative hen egg white lysozyme. Insights in BCWHs from bacteriophages to animals have provided concep...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2010.10.012
更新日期：2011-04-01 00:00:00
abstract：:The use of ultrafast laser pulses in surgery has allowed for unprecedented precision with minimal collateral damage to surrounding tissues. For these reasons, ultrafast laser nanosurgery, as an injury model, has gained tremendous momentum in experimental biology ranging from in vitro manipulations of subcellular struc...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2009.01.008
更新日期：2009-02-01 00:00:00
abstract：:Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2006.02.001
更新日期：2006-04-01 00:00:00
abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.06.019
更新日期：2014-12-01 00:00:00
abstract：:To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00144-0
更新日期：2000-12-01 00:00:00
abstract：:Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destru...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(95)80069-7
更新日期：1995-08-01 00:00:00
abstract：:Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2020.02.002
更新日期：2020-06-01 00:00:00
abstract：:During the last years, a lot of efforts have been put into developing novel sensors and biosensors systems for monitoring the occurrence of emerging pollutants in the environment. These analyses are often challenging because of the variety of physico-chemical properties of these substances, the extremely diluted conce...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2017.02.017
更新日期：2017-06-01 00:00:00
abstract：:Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90086-x
更新日期：1992-10-01 00:00:00
abstract：:Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00077-x
更新日期：2000-04-01 00:00:00
abstract：:The development of new strategies for the delivery of vaccine antigens or immune modulators to the mucosal tissue includes innovative approaches such as the use of genetically modified food microorganisms and plants. Even though the 'proof-of-concept' has recently been established for these two systems, key questions ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00255-x
更新日期：2001-10-01 00:00:00
abstract：:Many of the gene products of completely sequenced organisms are 'hypothetical' - they cannot be related to any previously characterized proteins - and so are of completely unknown function. Structural studies provide one means of obtaining functional information in these cases. A 'structural genomics' project has been...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(99)00063-4
更新日期：2000-02-01 00:00:00
abstract：:Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(02)00380-4
更新日期：2002-12-01 00:00:00
abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(03)00060-0
更新日期：2003-06-01 00:00:00
abstract：:The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet ke...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00075-6
更新日期：2000-04-01 00:00:00
abstract：:Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hos...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.12.029
更新日期：2012-10-01 00:00:00
abstract：:Scaffolds are used in tissue engineering as a matrix for the seeding and attachment of human cells. The creation of porosity in three-dimensional (3D) structures of scaffolds plays a critical role in cell proliferation, migration, and differentiation into the specific tissue while secreting extracellular matrix compon...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.04.005
更新日期：2011-10-01 00:00:00
abstract：:Much progress has been made in the development of techniques for constructing dense grids either of ligands, such as peptides and oligonucleotides, or of cloned nucleic acids. Such arrays are finding practical applications in the analysis of sequence variation and gene expression. Methods for carrying out large number...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80100-5
更新日期：1996-02-01 00:00:00
abstract：:Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yie...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2009.07.001
更新日期：2009-08-01 00:00:00
abstract：:To develop a viable H2 technology, production of H2 has to be significantly enlarged by using renewable resources. One option of generating H2 is the photosynthetic conversion of sunlight and water directly to H2 and O2. Photosystems and hydrogenases are currently being exploited for the design of efficient H2-produci...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/j.copbio.2011.01.006
更新日期：2011-06-01 00:00:00
abstract：:Fundamental biological processes such as cell-cell communication, signal transduction, molecular transport and energy conversion are performed by membrane proteins. These important proteins are studied best in their native environment, the lipid bilayer. The atomic force microscope (AFM) is the instrument of choice to...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.11.032
更新日期：2012-08-01 00:00:00
abstract：:New biological inspired methods were recently developed to recover precious metals from waste streams and to concomitantly produce palladium nanoparticles on bacteria, that is, bio-Pd. This technology offers a variety of opportunities, as the process can considered to be green, tunable, affordable and scalable. The na...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.01.007
更新日期：2012-08-01 00:00:00
abstract：:Phage display of proteins continues to be an important technology with a variety of applications. In the past year, advances have been made in coupling rational protein design with the power of the display selection process. In addition to the widely used filamentous phage, other bacteriophage surface expression syste...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80060-7
更新日期：1996-10-01 00:00:00
