Abstract:
:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogenase (PDH), pyruvate formate lyase (PFL), and pyruvate ferredoxin oxidoreductase (PFOR). For each of these pathways except PFL, we see examples where ethanol production has been engineered with a yield of >90% of the theoretical maximum. In each of these cases, this engineering was achieved mainly by modulating expression of native genes. We have not found an example where a thermophilic ethanol production pathway has been transferred to a non-ethanol-producing organism to produce ethanol at high yield. A key reason for the lack of transferability of ethanol production pathways is the current lack of understanding of the enzymes involved.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Olson DG,Sparling R,Lynd LRdoi
10.1016/j.copbio.2015.02.006subject
Has Abstractpub_date
2015-06-01 00:00:00pages
130-41eissn
0958-1669issn
1879-0429pii
S0958-1669(15)00025-7journal_volume
33pub_type
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