'Hybrid' processing strategies for expanding and improving the synthesis of renewable bioproducts.

abstract：:Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

journal_title：Current opinion in biotechnology

authors： Watanabe K

更新日期：2001-06-01 00:00:00

abstract：:The ecophysiology of microorganisms has been at the heart of microbial ecology since its early days, but only during the past decade have methods become available for cultivation-independent, direct identification of microorganisms in complex communities and for the simultaneous investigation of their activity and sub...

journal_title：Current opinion in biotechnology

authors： Wagner M,Nielsen PH,Loy A,Nielsen JL,Daims H

更新日期：2006-02-01 00:00:00

abstract：:The conservation and sustainable use of freshwater resources is of global importance. Microorganisms are not only the most abundant organisms in natural freshwater systems, but are also key players in ecological processes controlling water quality. Detailed knowledge of the diversity and function of microorganisms dwe...

journal_title：Current opinion in biotechnology

authors： Hahn MW

更新日期：2006-06-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activiti...

journal_title：Current opinion in biotechnology

authors： Allen DK,Young JD

更新日期：2020-08-01 00:00:00

abstract：:Fifteen years have passed since T-cell receptor (TCR) genes were identified (reviewed in [1]). Unlike the situation for antibodies, no direct structural information on the TCR proteins has been available for most of this time. Recently, however, the crystal structures of isolated alpha and beta chains were determined,...

journal_title：Current opinion in biotechnology

authors： Garcia KC,Teyton L

更新日期：1998-08-01 00:00:00

abstract：:RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

journal_title：Current opinion in biotechnology

authors： Rossi JJ

更新日期：1992-02-01 00:00:00

abstract：:There is growing scientific evidence supported by mechanistic and clinical studies that probiotics can provide health benefits. As probiotics are highly sensitive to many environmental factors, and because the propagation of many strains of intestinal origin is not straightforward, most commercial strains are selected...

journal_title：Current opinion in biotechnology

authors： Lacroix C,Yildirim S

更新日期：2007-04-01 00:00:00

abstract：:Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...

journal_title：Current opinion in biotechnology

authors： Martin CH,Kaufman DS

更新日期：2005-10-01 00:00:00

abstract：:One goal of plant genetic engineering is the manipulation of protein levels within crop plants. New insights into the ubiquitin-dependent proteolytic pathway provide potential novel ways of enhancing levels of desired proteins by synthesizing them as ubiquitin fusions, and reducing levels of undesired proteins by sele...

journal_title：Current opinion in biotechnology

authors： Hondred D,Vierstra RD

更新日期：1992-04-01 00:00:00

abstract：:Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understa...

journal_title：Current opinion in biotechnology

authors： Dougherty MJ,Arnold FH

更新日期：2009-08-01 00:00:00

abstract：:Fungal bioactive polysaccharides deriving mainly from the Basidiomycetes family (and some from the Ascomycetes) and medicinal mushrooms have been well known and widely used in far Asia as part of traditional diet and medicine, and in the last decades have been the core of intense research for the understanding and the...

journal_title：Current opinion in biotechnology

authors： Giavasis I

更新日期：2014-04-01 00:00:00

abstract：:Since the development of desorption electrospray ionization (DESI), many other ionization methods for ambient and atmospheric pressure mass spectrometry have been developed. Ambient ionization mass spectrometry has now been used for a wide variety of biological applications, including plant science, microbiology, neur...

journal_title：Current opinion in biotechnology

authors： Hsu CC,Dorrestein PC

更新日期：2015-02-01 00:00:00

abstract：:Over the next decade, the impact of library synthesis will play a major role in shortening the lead optimization phase of drug discovery. The prognosis for combinatorial chemistry to discover fundamentally different new classes of therapeutically active small molecules against some of the more difficult biological tar...

journal_title：Current opinion in biotechnology

authors： Myers PL

更新日期：1997-12-01 00:00:00

abstract：:The development of electrospray and matrix-assisted laser desorption mass spectrometry has provided protein chemists with tools for peptide and protein structure analysis with unprecedented sensitivity and molecular weight range. The two technologies can be viewed as competitive with respect to their molecular mass de...

journal_title：Current opinion in biotechnology

authors： Lee TD,Shively JE

更新日期：1991-02-01 00:00:00

abstract：:Fungal ligninolytic enzymes have broad biotechnological applications. Particularly laccases and certain fungal class II peroxidases from white-rot basidiomycetes are considered in degradation of persistent organic pollutants. Promising processes with reusable immobilized laccases in special reactors have been develope...

journal_title：Current opinion in biotechnology

authors： Kües U

更新日期：2015-06-01 00:00:00

abstract：:Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for ...

journal_title：Current opinion in biotechnology

authors： Besada-Lombana PB,McTaggart TL,Da Silva NA

更新日期：2018-10-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:The commercialization of high-precision microcalorimeters has led to a sharp increase in the acquisition and utilization of calorimetric data on the binding and folding reactions of proteins. This thermodynamic database, together with high-resolution structures of proteins and their complexes with ligands, has facilit...

journal_title：Current opinion in biotechnology

authors： Connelly PR

更新日期：1994-08-01 00:00:00

abstract：:Global aquaculture is projected to grow from 10 million to 20 million metric tons by the year 2000. To meet this projection, the aquaculture industry must bring some of its infectious disease problems under control without relying on antibiotics and chemotherapeutics. Thus, vaccines for fish and shellfish are being so...

journal_title：Current opinion in biotechnology

authors： Leong JA

更新日期：1993-06-01 00:00:00

abstract：:Organ-on-chip systems integrate microfluidic technology and living cells to study human physiology and pathophysiology. These human in vitro models are promising substitutes for animal testing, and their small scale enables precise control of culture conditions and high-throughput experiments, which would not be econo...

journal_title：Current opinion in biotechnology

authors： Luni C,Serena E,Elvassore N

更新日期：2014-02-01 00:00:00

abstract：:Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

journal_title：Current opinion in biotechnology

authors： Friedrich MW

更新日期：2006-02-01 00:00:00

abstract：:The metabolome, the collection of small molecules associated with an organism, is a growing subject of inquiry, with the data utilized for data-intensive systems biology, disease diagnostics, biomarker discovery, and the broader characterization of small molecules in mixtures. Owing to their close proximity to the fun...

journal_title：Current opinion in biotechnology

authors： Eghbalnia HR,Romero PR,Westler WM,Baskaran K,Ulrich EL,Markley JL

更新日期：2017-02-01 00:00:00

abstract：:The most significant feature of the current transgenic models of Alzheimer's disease continues to be the amyloid phenotype. In the past year, mice have been more extensively characterized in terms of the effect of amyloid accumulation on downstream events, such as neurodegeneration and behavioral changes, but the resu...

journal_title：Current opinion in biotechnology

authors： Duff K

更新日期：1998-12-01 00:00:00

abstract：:Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

journal_title：Current opinion in biotechnology

authors： Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

更新日期：2019-04-01 00:00:00

abstract：:In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

journal_title：Current opinion in biotechnology

authors： Gumpert J,Hoischen C

更新日期：1998-10-01 00:00:00

abstract：:Recent progress in synthetic and computational chemistry has made it possible to develop certain novel drug candidates. Drug candidates for genetic diseases, such as cancer, may also be designed on the basis of structural information obtained using X-ray analysis and NMR, as well as evidence from biological techniques...

journal_title：Current opinion in biotechnology

authors： lida H,Jia G,Lown JW

更新日期：1999-02-01 00:00:00

abstract：:Water deficit or drought stress is a major limitation to crop production globally. Plant breeders have used a wide range of technologies to successfully breed varieties that perform well under the growth conditions for their target environments but they are always seeking new opportunities to enhance rates of genetic ...

journal_title：Current opinion in biotechnology

authors： Langridge P,Reynolds MP

更新日期：2015-04-01 00:00:00

abstract：:The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medi...

journal_title：Current opinion in biotechnology

authors： Schwechheimer SK,Becker J,Wittmann C

更新日期：2018-12-01 00:00:00

abstract：:The impact of directed evolution and site-specific mutagenesis on the industrial utility of enzymatic catalysis through the modification of enzyme structure and function is clearly an important area of research in bioprocess engineering. High-throughput screening for novel or improved enzyme activities, both by more e...

journal_title：Current opinion in biotechnology

authors： Panke S,Wubbolts MG

更新日期：2002-04-01 00:00:00