Top value platform chemicals: bio-based production of organic acids.

abstract：:Recent advances in emerging food-processing technologies, such as high hydrostatic pressure or high-intensity electric field pulses, allow targeted and sophisticated modification and preservation of foods. We are beginning to understand the mechanisms involved in pressure inactivation of bacterial spores and have been...

journal_title：Current opinion in biotechnology

authors： Knorr D

更新日期：1999-10-01 00:00:00

abstract：:Phage display of proteins continues to be an important technology with a variety of applications. In the past year, advances have been made in coupling rational protein design with the power of the display selection process. In addition to the widely used filamentous phage, other bacteriophage surface expression syste...

journal_title：Current opinion in biotechnology

authors： Dunn IS

更新日期：1996-10-01 00:00:00

abstract：:Mitochondria are both morphologically and functionally diverse, and this variety is thought to have important biological ramifications. The development of methods to probe the properties of individual mitochondria is therefore of utmost importance. Recent advances have been made using in situ microscopy techniques and...

journal_title：Current opinion in biotechnology

authors： Fuller KM,Arriaga EA

更新日期：2003-02-01 00:00:00

abstract：:Adverse environmental conditions pose serious limitations to agricultural production. Classical biotechnological approaches towards increasing abiotic stress tolerance focus on boosting plant endogenous defence mechanisms. However, overexpression of regulatory elements or effectors is usually accompanied by growth han...

journal_title：Current opinion in biotechnology

authors： Cabello JV,Lodeyro AF,Zurbriggen MD

更新日期：2014-04-01 00:00:00

abstract：:Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...

journal_title：Current opinion in biotechnology

authors： Vanholme B,El Houari I,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:Extremolytes are small organic molecules, which protect cells under extreme, virtually inhabitable conditions. Their exceptional properties can be translated into health-promoting and therapeutic activities, which open an avenue of opportunities for the cosmetic, medical, and food industries. Supported by powerful app...

journal_title：Current opinion in biotechnology

authors： Becker J,Wittmann C

更新日期：2020-10-01 00:00:00

abstract：:Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

journal_title：Current opinion in biotechnology

authors： Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

更新日期：2019-04-01 00:00:00

abstract：:Combinatorial biocatalysis is an emerging technology in the field of drug discovery. The biocatalytic approach to combinatorial chemistry uses enzymatic, chemoenzymatic, and microbial transformations to generate libraries from lead compounds. Important recent advances in combinatorial biocatalysis include iterative de...

journal_title：Current opinion in biotechnology

authors： Altreuter DH,Clark DS

更新日期：1999-04-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...

journal_title：Current opinion in biotechnology

authors： Kildegaard HF,Baycin-Hizal D,Lewis NE,Betenbaugh MJ

更新日期：2013-12-01 00:00:00

abstract：:Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...

journal_title：Current opinion in biotechnology

authors： Jewett MC,Hofmann G,Nielsen J

更新日期：2006-04-01 00:00:00

abstract：:Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies...

journal_title：Current opinion in biotechnology

authors： Suter B,Kittanakom S,Stagljar I

更新日期：2008-08-01 00:00:00

abstract：:Efforts to develop culture technologies capable of eliciting robust human blood stem cell growth have met with limited success. Considering that adult stem cell cultures are complex systems, comprising multiple cell types with dynamically changing intracellular signalling environments and cellular compositions, this i...

journal_title：Current opinion in biotechnology

authors： Kirouac DC,Zandstra PW

更新日期：2006-10-01 00:00:00

abstract：:In the past two decades, biological nanopores have been developed and explored for use in sensing applications as a result of their exquisite sensitivity and easily engineered, reproducible, and economically manufactured structures. Nanopore sensing has been shown to differentiate between highly similar analytes, meas...

journal_title：Current opinion in biotechnology

authors： Schmidt J

更新日期：2016-06-01 00:00:00

abstract：:Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

journal_title：Current opinion in biotechnology

authors： Wagner H,Heit A,Schmitz F,Bauer S

更新日期：2004-12-01 00:00:00

abstract：:Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nu...

journal_title：Current opinion in biotechnology

authors： Houlihan G,Arangundy-Franklin S,Holliger P

更新日期：2017-12-01 00:00:00

abstract：:Clinical testing of therapies for sepsis that target tumor necrosis factor and interleukin-1 is currently in progress. It is now clear from early clinical results that patients display a heterogeneous response to anti-cytokine therapies that may be related to the stage or severity of disease. Experimental results show...

journal_title：Current opinion in biotechnology

authors： Russell DA,Thompson RC

更新日期：1993-12-01 00:00:00

abstract：:Signal transduction is the process of converting one kind of signal or stimulus into another, with the goal of processing external or internal signals into diverse functions. In recent years, progress has been made in identifying signaling pathways, investigating cross-talk and feedbacks in signaling modules, analyzin...

journal_title：Current opinion in biotechnology

authors： Kiel C,Serrano L

更新日期：2012-06-01 00:00:00

abstract：:Microbes have long been adapted for the biosynthetic production of useful compounds. There is increasing demand for the rapid and cheap microbial production of diverse molecules in an industrial setting. Microbes can now be designed and engineered for a particular biosynthetic purpose, thanks to recent developments in...

journal_title：Current opinion in biotechnology

authors： Quin MB,Schmidt-Dannert C

更新日期：2014-10-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...

journal_title：Current opinion in biotechnology

authors： Sittinger M,Hutmacher DW,Risbud MV

更新日期：2004-10-01 00:00:00

abstract：:Technoeconomic analysis (TEA) is an approach for conducting process design and simulation, informed by empirical data, to estimate capital costs, operating costs, mass balances, and energy balances for a commercial scale biorefinery. TEA serves as a useful method to screen potential research priorities, identify cost ...

journal_title：Current opinion in biotechnology

authors： Scown CD,Baral NR,Yang M,Vora N,Huntington T

更新日期：2021-01-18 00:00:00

abstract：:The development of new strategies for the delivery of vaccine antigens or immune modulators to the mucosal tissue includes innovative approaches such as the use of genetically modified food microorganisms and plants. Even though the 'proof-of-concept' has recently been established for these two systems, key questions ...

journal_title：Current opinion in biotechnology

authors： Mercenier A,Wiedermann U,Breiteneder H

更新日期：2001-10-01 00:00:00

abstract：:Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...

journal_title：Current opinion in biotechnology

authors： Park D,Swayambhu G,Pfeifer BA

更新日期：2020-12-01 00:00:00

abstract：:Contamination of the vadose zone with various pollutants is a world-wide problem, and often technical or economic constraints impose remediation without excavation. In situ bioremediation in the vadose zone by bioventing has become a standard remediation technology for light spilled petroleum products. In this review,...

journal_title：Current opinion in biotechnology

authors： Höhener P,Ponsin V

更新日期：2014-06-01 00:00:00

abstract：:Growing interest in RNAs for biomedical, agricultural, and other applications has necessitated the development of methods for producing oligoribonucleotides in milligram, or larger, quantities. Recent research on in vitro transcription has focused on optimizing solution conditions and the use of immobilized templates....

journal_title：Current opinion in biotechnology

authors： Davis RH

更新日期：1995-04-01 00:00:00

abstract：:Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

journal_title：Current opinion in biotechnology

authors： Junker BH

更新日期：2014-04-01 00:00:00

abstract：:Considerable progress has been made in understanding the relationship between local amino acid sequence and local protein structure. Recent highlights include numerous studies of the structures adopted by short peptides, new approaches to correlating sequence patterns with structure patterns, and folding simulations u...

journal_title：Current opinion in biotechnology

authors： Bystroff C,Simons KT,Han KF,Baker D

更新日期：1996-08-01 00:00:00

abstract：:During the last years, a lot of efforts have been put into developing novel sensors and biosensors systems for monitoring the occurrence of emerging pollutants in the environment. These analyses are often challenging because of the variety of physico-chemical properties of these substances, the extremely diluted conce...

journal_title：Current opinion in biotechnology

authors： Sanchís J,Llorca M,Barceló D,Farré M

更新日期：2017-06-01 00:00:00

abstract：:A growing body of evidence indicates that many cellular reactions within metabolic pathways are catalyzed not by free-floating 'soluble' enzymes, but via one or more membrane-associated multienzyme complexes. This type of macromolecular organization has important implications for the overall efficiency, specificity, a...

journal_title：Current opinion in biotechnology

authors： Conrado RJ,Varner JD,DeLisa MP

更新日期：2008-10-01 00:00:00

abstract：:The federal government is critically examining its responsibilities and opportunities for bringing the new field of gene therapy to fruition and for assuring public confidence in this new area of biomedicine. The evolving mechanisms for review and regulation in human gene transfer studies in the United States are bein...

journal_title：Current opinion in biotechnology

authors： Friedmann T,Noguchi P,Mickelson C

更新日期：2001-06-01 00:00:00