Abstract:
:Efforts to develop culture technologies capable of eliciting robust human blood stem cell growth have met with limited success. Considering that adult stem cell cultures are complex systems, comprising multiple cell types with dynamically changing intracellular signalling environments and cellular compositions, this is not surprising. Typically treated as single-input single-output systems, adult stem cell cultures are better described as complex, non-linear, multiple-input multiple-output systems wherein the proliferation of subpopulations of cells leads to the formation of intercellular endogenously secreted protein interaction networks. Genomic and proteomic tools need to be applied to generate high-throughput (and ideally high-content) biological measurements of stem cell culture evolution. Datasets describing cellular interaction networks need to be integrated into predictive models of in vitro stem cell development. Ultimately, such models will serve as a starting point for the rational design of blood stem cell expansion bioprocesses utilizing dynamic system perturbations to achieve the preferential expansion of target cell populations.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Kirouac DC,Zandstra PWdoi
10.1016/j.copbio.2006.07.003subject
Has Abstractpub_date
2006-10-01 00:00:00pages
538-47issue
5eissn
0958-1669issn
1879-0429pii
S0958-1669(06)00103-0journal_volume
17pub_type
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2006.08.001
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.12.001
更新日期:2014-06-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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更新日期:1998-10-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2005.12.007
更新日期:2006-02-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2006.02.001
更新日期:2006-04-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0958-1669(95)80112-x
更新日期:1995-12-01 00:00:00
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journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2019.01.011
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journal_title:Current opinion in biotechnology
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doi:10.1016/s0958-1669(00)00169-5
更新日期:2001-02-01 00:00:00
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(99)00015-4
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2009.07.001
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journal_title:Current opinion in biotechnology
pub_type: 临床试验,杂志文章,评审
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2017.09.006
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journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2013.03.021
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2010.12.003
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abstract::The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medi...
journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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abstract::Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.02.011
更新日期:2017-08-01 00:00:00