High-throughput system-wide engineering and screening for microbial biotechnology.

Abstract:

:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their experiments to the nanoliter and picoliter scale and the single-cell level. In this review, we summarize different high-throughput system-wide genome engineering and screening strategies for microbes. More specifically, we will emphasize the use of multiplex automated genome evolution (MAGE) and CRISPR/Cas systems for high-throughput genome engineering and the application of (lab-on-chip) nanoreactors for high-throughput single-cell or population screening.

journal_name

Curr Opin Biotechnol

authors

Vervoort Y,Linares AG,Roncoroni M,Liu C,Steensels J,Verstrepen KJ

doi

10.1016/j.copbio.2017.02.011

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

120-125

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(16)30277-4

journal_volume

46

pub_type

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