Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project.


:The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet kept up to this pace. Recent progress in large-scale insertional mutagenesis opens new possibilities for functional genomics in Arabidopsis. The number of T-DNA and transposon insertion lines from different laboratories will soon represent insertions into most Arabidopsis genes. Vast resources of gene knockouts are becoming available that can be subjected to different types of reverse genetics screens to deduce the functions of the sequenced genes.


Curr Opin Biotechnol


Parinov S,Sundaresan V




Has Abstract


2000-04-01 00:00:00














  • Microbial production of diols as platform chemicals: recent progresses.

    abstract::Diols are chemicals with two hydroxyl groups which have a wide range of appealing applications as chemicals and fuels. In particular, four diol compounds, namely 1,3-propanediol (1,3-PDO), 1,2-propanediol (1,2-PDO), 2,3-butanediol (2,3-BDO) and 1,4-butanediol (1,4-BDO) can be biotechnologically produced by direct micr...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Zeng AP,Sabra W

    更新日期:2011-12-01 00:00:00

  • Towards real-time monitoring of therapeutic protein quality in mammalian cell processes.

    abstract::Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pais DA,Carrondo MJ,Alves PM,Teixeira AP

    更新日期:2014-12-01 00:00:00

  • Epigenomic technologies for deciphering circulating tumor DNA.

    abstract::Tumor-derived DNA, found in body fluids (liquid biopsy) of cancer patients as part of cell-free DNA (cfDNA), lends itself for noninvasive cancer detection and monitoring. Advantages of cfDNA as analytical target have evoked a burst of sophisticated techniques, providing clinically relevant information. Each cell type ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Daniūnaitė K,Jarmalaitė S,Kriukienė E

    更新日期:2019-02-01 00:00:00

  • Cranberry components for the therapy of infectious disease.

    abstract::Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

    更新日期:2012-04-01 00:00:00

  • Biological function made crystal clear - annotation of hypothetical proteins via structural genomics.

    abstract::Many of the gene products of completely sequenced organisms are 'hypothetical' - they cannot be related to any previously characterized proteins - and so are of completely unknown function. Structural studies provide one means of obtaining functional information in these cases. A 'structural genomics' project has been...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Eisenstein E,Gilliland GL,Herzberg O,Moult J,Orban J,Poljak RJ,Banerjei L,Richardson D,Howard AJ

    更新日期:2000-02-01 00:00:00

  • Protein interaction databases.

    abstract::Life depends on the interaction of proteins. The availability of the complete human genome sequence has highlighted the need for a tool to analyse protein interactions and several databases have been compiled for this purpose. These databases document, categorize, and analyze interacting proteins and the cellular func...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Xenarios I,Eisenberg D

    更新日期:2001-08-01 00:00:00

  • Technological applications arising from the interactions of DNA bases with metal ions.

    abstract::An intense interest has grown in the unique interactions of nucleic acids with metal ions, which lead to the formation of metal-base pairs and the generation of fluorescent nanomaterials. In this review, different types of metal-base pairs, especially those formed from naturally occurring nucleosides, are described wi...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Park KS,Park HG

    更新日期:2014-08-01 00:00:00

  • Stable isotope probing of carbon flow in the plant holobiont.

    abstract::Microbial communities associated with a plant host, constituting a holobiont, affect the physiology and growth of the plant via metabolites that are mainly derived from their photosynthates. The structure and function of active microbial communities that assimilate root exudates can be tracked by using stable isotope ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Haichar FEZ,Heulin T,Guyonnet JP,Achouak W

    更新日期:2016-10-01 00:00:00

  • Steps towards 'drop-in' biofuels: focusing on metabolic pathways.

    abstract::The past decade has witnessed rapid advance in microbial production of 'drop-in' biofuels from renewable resources. Various biosynthetic pathways have been constructed to produce biofuels with diverse structures, and multiple metabolic engineering strategies have been developed to increase biofuel titers, yields, prod...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Jiang W,Gu P,Zhang F

    更新日期:2018-10-01 00:00:00

  • The use of adenoviral vectors for gene therapy and gene transfer in vivo.

    abstract::Adenoviral vectors have proven to be excellent vehicles for gene delivery in vivo to a wide range of cell types. These vectors have been used to transfer genes such as CFTR to correct the defect in cystic fibrosis and, more recently, to supply serum blood factors and genetically modify tumors to enhance therapy. ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bramson JL,Graham FL,Gauldie J

    更新日期:1995-10-01 00:00:00

  • Biosynthesis of fatty acid-derived hydrocarbons: perspectives on enzymology and enzyme engineering.

    abstract::Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels. In the past decade, novel hydrocar...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Liu K,Li S

    更新日期:2020-04-01 00:00:00

  • Silent constraints: the hidden challenges faced in plant metabolic engineering.

    abstract::Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lynch JH,Huang XQ,Dudareva N

    更新日期:2021-01-08 00:00:00

  • Biological hydrogen production by dark fermentation: challenges and prospects towards scaled-up production.

    abstract::Among different technologies of hydrogen production, bio-hydrogen production exhibits perhaps the greatest potential to replace fossil fuels. Based on recent research on dark fermentative hydrogen production, this article reviews the following aspects towards scaled-up application of this technology: bioreactor develo...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: RenNanqi,GuoWanqian,LiuBingfeng,CaoGuangli,DingJie

    更新日期:2011-06-01 00:00:00

  • Designer microbes for biosynthesis.

    abstract::Microbes have long been adapted for the biosynthetic production of useful compounds. There is increasing demand for the rapid and cheap microbial production of diverse molecules in an industrial setting. Microbes can now be designed and engineered for a particular biosynthetic purpose, thanks to recent developments in...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Quin MB,Schmidt-Dannert C

    更新日期:2014-10-01 00:00:00

  • Novel applications of the ubiquitin-dependent proteolytic pathway in plant genetic engineering.

    abstract::One goal of plant genetic engineering is the manipulation of protein levels within crop plants. New insights into the ubiquitin-dependent proteolytic pathway provide potential novel ways of enhancing levels of desired proteins by synthesizing them as ubiquitin fusions, and reducing levels of undesired proteins by sele...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hondred D,Vierstra RD

    更新日期:1992-04-01 00:00:00

  • Gene expression using Xenopus oocytes.

    abstract::Xenopus oocytes have been used as a surrogate genetic system for the study of many facets of gene expression. Some recent salient examples where injected oocytes proved to be indispensable for the analysis of transcription, translation, RNA/protein transport, and ion channel formation are described. ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Richter JD

    更新日期:1991-10-01 00:00:00

  • Dynamic resolution and stereoinversion of secondary alcohols by chemo-enzymatic processes.

    abstract::To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Azerad R,Buisson D

    更新日期:2000-12-01 00:00:00

  • Enantioselective biocatalysis optimized by directed evolution.

    abstract::Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactio...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Jaeger KE,Eggert T

    更新日期:2004-08-01 00:00:00

  • Targeting split vaccines to the endosome improves vaccination.

    abstract::Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wagner H,Heit A,Schmitz F,Bauer S

    更新日期:2004-12-01 00:00:00

  • Advances in monitoring environmental microbes.

    abstract::Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hazen TC,Rocha AM,Techtmann SM

    更新日期:2013-06-01 00:00:00

  • Novel biotechnological approaches to produce biological compounds: challenges and opportunities for science communication.

    abstract::Novel biotechnological approaches such as Metabolic Engineering (ME) and New Plant Breeding Techniques (NPBTs) are currently being developed to produce biological compounds for food and non-food products. NPBTs span a range of methods for in vivo production in crops, some of which are classified as GMOs while others a...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pei L,Schmidt M

    更新日期:2019-04-01 00:00:00

  • Poxviruses as expression vectors.

    abstract::Poxviruses are widely used for the cytoplasmic expression of recombinant genes in mammalian cells. Recent improvements allow high expression and simplify the integration of multiple foreign genes. Vaccinia virus mutants and other poxviruses that undergo abortive infection in mammalian cells are receiving special atten...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Carroll MW,Moss B

    更新日期:1997-10-01 00:00:00

  • APOE is a major susceptibility gene for Alzheimer's disease.

    abstract::The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Roses AD,Saunders AM

    更新日期:1994-12-01 00:00:00

  • Protein fragment complementation strategies for biochemical network mapping.

    abstract::The organization of biochemical networks that make up the living cell can be defined by studying the dynamics of protein-protein interactions. To this end, experimental strategies based on protein fragment complementation assays (PCAs) have been used to map biochemical networks and to identify novel components of thes...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Michnick SW

    更新日期:2003-12-01 00:00:00

  • Antibody discovery: phage display.

    abstract::Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kretzschmar T,von Rüden T

    更新日期:2002-12-01 00:00:00

  • Engineering nanoscale protein compartments for synthetic organelles.

    abstract::Advances in metabolic engineering have given rise to the biological production of novel fuels and chemicals, but yields are often low without significant optimization. One generalizable solution is to create a specialized organelle for the sequestration of engineered metabolic pathways. Bacterial microcompartments are...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kim EY,Tullman-Ercek D

    更新日期:2013-08-01 00:00:00

  • Advances in optical detection strategies for reporter signal measurements.

    abstract::Many recent advances in bioreporter technology focus on challenges related to bioengineering, yet in many applications implementation of optical signal measurement is equally susceptible to improvement. For bioluminescent bioreporters, one area of effort lies in the development of semiconductor chip-based detector mod...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wells M

    更新日期:2006-02-01 00:00:00

  • Protein secretion in bacteria.

    abstract::Most secretory proteins are synthesized as precursors with an amino-terminal signal peptide. Genetic identification of proteins essential for signal peptide dependent translocation to the Escherichia coli periplasm has led to the biochemical dissection of the secretion pathway. Additional mechanisms exist in Gram-nega...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Gennity JM,Inouye M

    更新日期:1991-10-01 00:00:00

  • Understanding cellular networks to improve hematopoietic stem cell expansion cultures.

    abstract::Efforts to develop culture technologies capable of eliciting robust human blood stem cell growth have met with limited success. Considering that adult stem cell cultures are complex systems, comprising multiple cell types with dynamically changing intracellular signalling environments and cellular compositions, this i...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kirouac DC,Zandstra PW

    更新日期:2006-10-01 00:00:00

  • Systems biology approach to bioremediation.

    abstract::Bioremediation has historically been approached as a 'black box' in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Chakraborty R,Wu CH,Hazen TC

    更新日期:2012-06-01 00:00:00