Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project.

abstract：:Plants synthesize a myriad of secondary metabolites (SMs) that are derived from central or primary metabolism. While these so-called natural products have been targets for plant metabolic engineering attempts for many years, the immense value of manipulating the interface between committed steps in secondary metabolis...

journal_title：Current opinion in biotechnology

authors： Aharoni A,Galili G

更新日期：2011-04-01 00:00:00

abstract：:The possibility of generating electricity with microbial fuel cells has been recognized for some time, but practical applications have been slow to develop. The recent development of a microbial fuel cell that can harvest electricity from the organic matter stored in marine sediments has demonstrated the feasibility o...

journal_title：Current opinion in biotechnology

authors： Lovley DR

更新日期：2006-06-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...

journal_title：Current opinion in biotechnology

authors： Olson DG,Sparling R,Lynd LR

更新日期：2015-06-01 00:00:00

abstract：:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

journal_title：Current opinion in biotechnology

authors： Eckart MR,Bussineau CM

更新日期：1996-10-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...

journal_title：Current opinion in biotechnology

authors： Ogawa J,Shimizu S

更新日期：2002-08-01 00:00:00

abstract：:RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

journal_title：Current opinion in biotechnology

authors： Rossi JJ

更新日期：1992-02-01 00:00:00

abstract：:A mechanistic understanding of gene regulatory network dynamics requires quantitative single-cell data of multiple network components in response to well-defined perturbations. Recent advances in the development of fluorescent biomarkers for proteins, detection of RNA and interactions, microfluidic technology, and hig...

journal_title：Current opinion in biotechnology

authors： Selimkhanov J,Hasty J,Tsimring LS

更新日期：2012-02-01 00:00:00

abstract：:Polyphenols are secondary metabolites produced by higher plants, which play multiple essential roles in plant physiology and have potential healthy properties on human organism, mainly as antioxidants, anti-allergic, anti-inflammatory, anticancer, antihypertensive, and antimicrobial agents. In the present review the a...

journal_title：Current opinion in biotechnology

authors： Daglia M

更新日期：2012-04-01 00:00:00

abstract：:Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for ...

journal_title：Current opinion in biotechnology

authors： Besada-Lombana PB,McTaggart TL,Da Silva NA

更新日期：2018-10-01 00:00:00

abstract：:Plant-derived diterpenoids are indispensable to plant development, stress-resistance and interaction with environmental microorganisms. Besides significant roles in plant fitness and adaption, many bioactivities beneficial to human beings are also found in diterpenoids from terrestrial plants. However, these high-valu...

journal_title：Current opinion in biotechnology

authors： Shao J,Sun Y,Liu H,Wang Y

更新日期：2020-10-05 00:00:00

abstract：:Plant-based foods offer a wide range of nutrients that are essential for human and animal health. Among these nutrients, iron stands out as one of the most important micronutrients. Increasing the iron content in many staple and non-staple plant foods continues to be a goal of many scientists around the world. However...

journal_title：Current opinion in biotechnology

authors： Vasconcelos MW,Gruissem W,Bhullar NK

更新日期：2017-04-01 00:00:00

abstract：:In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these display techniques ha...

journal_title：Current opinion in biotechnology

authors： Amstutz P,Forrer P,Zahnd C,Plückthun A

更新日期：2001-08-01 00:00:00

abstract：:Recent advances in the use of fluorescence spectroscopy to study protein interactions have primarily involved combinations of classic fluorescence techniques, novel probe and coupling chemistries, and advances in laser excitation and detection capabilities. For example, new coupling strategies for fluorescent probes h...

journal_title：Current opinion in biotechnology

authors： Brown MP,Royer C

更新日期：1997-02-01 00:00:00

abstract：:Organ-on-chip systems integrate microfluidic technology and living cells to study human physiology and pathophysiology. These human in vitro models are promising substitutes for animal testing, and their small scale enables precise control of culture conditions and high-throughput experiments, which would not be econo...

journal_title：Current opinion in biotechnology

authors： Luni C,Serena E,Elvassore N

更新日期：2014-02-01 00:00:00

abstract：:Microbial communities within anaerobic ecosystems have evolved to degrade and recycle carbon throughout the earth. A number of strains have been isolated from anaerobic microbial communities, which are rich in carbohydrate active enzymes (CAZymes) to liberate fermentable sugars from crude plant biomass (lignocellulose...

journal_title：Current opinion in biotechnology

authors： Lillington SP,Leggieri PA,Heom KA,O'Malley MA

更新日期：2020-04-01 00:00:00

abstract：:The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowled...

journal_title：Current opinion in biotechnology

authors： Lee B,Vasmatzis G

更新日期：1997-08-01 00:00:00

abstract：:Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nu...

journal_title：Current opinion in biotechnology

authors： Houlihan G,Arangundy-Franklin S,Holliger P

更新日期：2017-12-01 00:00:00

abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...

journal_title：Current opinion in biotechnology

authors： McGrath SP,Zhao FJ

更新日期：2003-06-01 00:00:00

abstract：:Recombinant proteins that have survived the challenges of process development and clinical trials are becoming blockbuster medical products. Growth factors, enzymes and antibodies are being improved by mutational approaches, fused with other proteins, and even chemically modified in vitro. Drug development and testing...

journal_title：Current opinion in biotechnology

authors： Koths K

更新日期：1995-12-01 00:00:00

abstract：:Over the next decade, the impact of library synthesis will play a major role in shortening the lead optimization phase of drug discovery. The prognosis for combinatorial chemistry to discover fundamentally different new classes of therapeutically active small molecules against some of the more difficult biological tar...

journal_title：Current opinion in biotechnology

authors： Myers PL

更新日期：1997-12-01 00:00:00

abstract：:Various strategies have been developed to exploit plants as bioreactors for the production of pharmaceutical antibodies, to engineer antibody-mediated pathogen resistance or to alter the plant phenotype by immunomodulation. Recent research developments focus on the fine-tuning of expression systems and the detailed ch...

journal_title：Current opinion in biotechnology

authors： Stoger E,Sack M,Fischer R,Christou P

更新日期：2002-04-01 00:00:00

abstract：:Many significant advances have occurred recently in the field of protein electrophoresis and related technologies. Improvements have been made in capillary electrophoresis in apparatus design, detection, and capillary modification with coatings and fillers. The transfer of proteins to a blot, the capacity to analyze a...

journal_title：Current opinion in biotechnology

authors： Zewert TE,Harrington MG

更新日期：1993-02-01 00:00:00

abstract：:Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound protein without using harsh or denaturing elution conditions. The development of methods to identify monoclonal antibodies that bind antigens tightly, but rele...

journal_title：Current opinion in biotechnology

authors： Burgess RR,Thompson NE

更新日期：2002-08-01 00:00:00

abstract：:Biological pulping has the potential to improve the quality of pulp, properties of paper and to reduce energy costs and environmental impact relative to traditional pulping operations. It has been suggested that energy savings alone could make the process economically viable. Other benefits include improved burst stre...

journal_title：Current opinion in biotechnology

authors： Breen A,Singleton FL

更新日期：1999-06-01 00:00:00

abstract：:Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

journal_title：Current opinion in biotechnology

authors： Watanabe K

更新日期：2001-06-01 00:00:00

abstract：:Patient-derived xenografts (PDXs) are obtained by transplanting fragments of a patient's tumour into immunodeficient mice. Growth and propagation of PDXs allows correlating therapeutic response in vivo with extensive, multi-dimensional molecular annotation, leading to identification of predictive biomarkers. PDXs are ...

journal_title：Current opinion in biotechnology

authors： Invrea F,Rovito R,Torchiaro E,Petti C,Isella C,Medico E

更新日期：2020-06-01 00:00:00

abstract：:Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activiti...

journal_title：Current opinion in biotechnology

authors： Allen DK,Young JD

更新日期：2020-08-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00