Abstract:
:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Gygi SP,Rist B,Aebersold Rdoi
10.1016/s0958-1669(00)00116-6subject
Has Abstractpub_date
2000-08-01 00:00:00pages
396-401issue
4eissn
0958-1669issn
1879-0429pii
S0958-1669(00)00116-6journal_volume
11pub_type
杂志文章,评审abstract::Microfluidic systems have emerged as revolutionary new platform technologies for a range of applications, from consumer products such as inkjet printer cartridges to lab-on-a-chip diagnostic systems. Recent developments have opened the door to a new set of opportunities for microfluidic systems, in the field of tissue...
journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2011.05.512
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abstract::Fatty acid oxidation pathways are attractive for metabolic engineering purposes due to their cyclic nature as well as their reactions that allow for the selective functionalization of alkyl chains. These characteristics allow for the production of various chemicals, such as alcohols, alkanes, ketones and hydroxyacids,...
journal_title:Current opinion in biotechnology
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abstract::Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...
journal_title:Current opinion in biotechnology
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abstract::The phytodegradation of organic compounds can take place inside the plant or within the rhizosphere of the plant. Many different compounds and classes of compounds can be removed from the environment by this method, including solvents in groundwater, petroleum and aromatic compounds in soils, and volatile compounds in...
journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2004.04.006
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abstract::Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2005.07.007
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abstract::Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0958-1669(92)90086-x
更新日期:1992-10-01 00:00:00
abstract::Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applicat...
journal_title:Current opinion in biotechnology
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abstract::Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2019.03.015
更新日期:2019-06-01 00:00:00
abstract::Patient-derived xenografts (PDXs) are obtained by transplanting fragments of a patient's tumour into immunodeficient mice. Growth and propagation of PDXs allows correlating therapeutic response in vivo with extensive, multi-dimensional molecular annotation, leading to identification of predictive biomarkers. PDXs are ...
journal_title:Current opinion in biotechnology
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abstract::Broad application of microbial fuel cells will require substantial increases in current density. A better understanding of the microbiology of these systems may help. Recent studies have greatly expanded the range of microorganisms known to function either as electrode-reducing microorganisms at the anode or as electr...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2008.10.005
更新日期:2008-12-01 00:00:00
abstract::Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(05)80109-0
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abstract::Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2006.02.001
更新日期:2006-04-01 00:00:00
abstract::The use of ultrafast laser pulses in surgery has allowed for unprecedented precision with minimal collateral damage to surrounding tissues. For these reasons, ultrafast laser nanosurgery, as an injury model, has gained tremendous momentum in experimental biology ranging from in vitro manipulations of subcellular struc...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2009.01.008
更新日期:2009-02-01 00:00:00
abstract::Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2018.11.012
更新日期:2019-04-01 00:00:00
abstract::Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.05.006
更新日期:2013-10-01 00:00:00
abstract::Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.02.007
更新日期:2013-12-01 00:00:00
abstract::Nutraceuticals are important natural bioactive compounds that confer health-promoting and medical benefits to humans. Globally growing demands for value-added nutraceuticals for prevention and treatment of human diseases have rendered nutraceuticals a multi-billion dollar market. However, supply limitations and extrac...
journal_title:Current opinion in biotechnology
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doi:10.1016/j.copbio.2015.11.003
更新日期:2016-02-01 00:00:00
abstract::To develop a viable H2 technology, production of H2 has to be significantly enlarged by using renewable resources. One option of generating H2 is the photosynthetic conversion of sunlight and water directly to H2 and O2. Photosystems and hydrogenases are currently being exploited for the design of efficient H2-produci...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章
doi:10.1016/j.copbio.2011.01.006
更新日期:2011-06-01 00:00:00
abstract::Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2011.10.009
更新日期:2012-04-01 00:00:00
abstract::Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.06.019
更新日期:2014-12-01 00:00:00
abstract::Since the development of desorption electrospray ionization (DESI), many other ionization methods for ambient and atmospheric pressure mass spectrometry have been developed. Ambient ionization mass spectrometry has now been used for a wide variety of biological applications, including plant science, microbiology, neur...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.07.005
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abstract::Over the review period, a significant amount of literature has been published documenting the impact of biofilms on engineered and biomedical systems. Reactor systems and analytical techniques have evolved to study the molecular chemistry and microbial ecology within biofilm layers only tens of micrometers thick, and ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0958-1669(93)90125-g
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abstract::Based on projections for global population growth, current techniques for improving agricultural yields will not be able to address future demands for major food crops. Improving photosynthetic efficiency by engineering carbon fixation has been identified as one of the most important approaches for increasing agricult...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.01.004
更新日期:2017-08-01 00:00:00
abstract::Lactococcus lactis is the primary model organism for lactic acid bacteria (LAB) and is widely used in the production of fermented dairy products. In recent years there has been increasing interest in strains isolated from non-dairy environments, as these exhibit a high metabolic diversity and have unique flavour-formi...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2006.02.007
更新日期:2006-04-01 00:00:00
abstract::Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2018.08.011
更新日期:2019-02-01 00:00:00
abstract::The selection of an expression platform for recombinant biopharmaceuticals is often centered upon suitable product titers and critical quality attributes, including post-translational modifications. Although notable differences between microbial, yeast, plant, and mammalian host systems exist, recent advances have gre...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.06.007
更新日期:2014-12-01 00:00:00
abstract::The development of contrast agents that can be localized to a particular tissue or cellular epitope will potentially allow the noninvasive visualization and characterization of a variety of disease states. Recent advances have been made in the field of molecular imaging with magnetic resonance imaging and ultrasound a...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2004.11.001
更新日期:2005-02-01 00:00:00
abstract::Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UB...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.09.006
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abstract::Synthetic building blocks bearing hydroxylated chiral centers are important targets for biocatalysis. Many C-C bond forming enzymes have recently been investigated for new applications and new strategies towards the synthesis of natural products and related oxygenated compounds. Several old catalysts have been studied...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(01)00265-8
更新日期:2001-12-01 00:00:00
abstract::Metabolic flux analysis (MFA) represents a powerful tool for systems biology research on eukaryotic cells. This review describes recent advances, the challenges as well as applications of metabolic flux analysis comprising fungi, mammalian cells and plants. While MFA is widely established and applied in microorganisms...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2010.01.011
更新日期:2010-02-01 00:00:00