Targeting split vaccines to the endosome improves vaccination.

abstract：:The phytodegradation of organic compounds can take place inside the plant or within the rhizosphere of the plant. Many different compounds and classes of compounds can be removed from the environment by this method, including solvents in groundwater, petroleum and aromatic compounds in soils, and volatile compounds in...

journal_title：Current opinion in biotechnology

authors： Newman LA,Reynolds CM

更新日期：2004-06-01 00:00:00

abstract：:Regulatory networks of transcription factors and signaling molecules lie at the heart of development. Their architecture implements logic functions whose execution propels cells from one regulatory state to the next, thus driving development forward. As an example of a subcircuit that translates transcriptional input ...

journal_title：Current opinion in biotechnology

authors： Materna SC,Davidson EH

更新日期：2007-08-01 00:00:00

abstract：:Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hos...

journal_title：Current opinion in biotechnology

authors： Yuzawa S,Kim W,Katz L,Keasling JD

更新日期：2012-10-01 00:00:00

abstract：:Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...

journal_title：Current opinion in biotechnology

authors： Marquardt LM,Sakiyama-Elbert SE

更新日期：2013-10-01 00:00:00

abstract：:Is structure, rather than sequence, the key to the successful generation of truly novel proteins? While protein evolution by homologous recombination has become an established tool to explore confined regions in sequence space, the generation of functional hybrid proteins by homology-independent methods further expand...

journal_title：Current opinion in biotechnology

authors： Lutz S,Benkovic SJ

更新日期：2000-08-01 00:00:00

abstract：:The information encoded in the base sequence of DNA provides substantial structural and functional information for the instructive self-assembly of one-dimensional (1D) functional DNA nanostructures. The hybridization chain reaction (HCR) and the formation of HCR-stimulated DNAzyme nanochains are presented, as a means...

journal_title：Current opinion in biotechnology

authors： Wang F,Willner B,Willner I

更新日期：2013-08-01 00:00:00

abstract：:Plant-based foods offer a wide range of nutrients that are essential for human and animal health. Among these nutrients, iron stands out as one of the most important micronutrients. Increasing the iron content in many staple and non-staple plant foods continues to be a goal of many scientists around the world. However...

journal_title：Current opinion in biotechnology

authors： Vasconcelos MW,Gruissem W,Bhullar NK

更新日期：2017-04-01 00:00:00

abstract：:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for preci...

journal_title：Current opinion in biotechnology

authors： Tolle F,Stücheli P,Fussenegger M

更新日期：2019-10-01 00:00:00

abstract：:Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in severa...

journal_title：Current opinion in biotechnology

authors： Krishnan M,Namasivayam V,Lin R,Pal R,Burns MA

更新日期：2001-02-01 00:00:00

abstract：:The federal government is critically examining its responsibilities and opportunities for bringing the new field of gene therapy to fruition and for assuring public confidence in this new area of biomedicine. The evolving mechanisms for review and regulation in human gene transfer studies in the United States are bein...

journal_title：Current opinion in biotechnology

authors： Friedmann T,Noguchi P,Mickelson C

更新日期：2001-06-01 00:00:00

abstract：:Solventogenic clostridia are strictly anaerobic, endospore forming bacteria that produce a large array of primary metabolites, like butanol, by anaerobically degrading simple and complex carbohydrates, including cellulose and hemicellulose. Two genomes have been sequenced and some genetic tools have been developed, bu...

journal_title：Current opinion in biotechnology

authors： Papoutsakis ET

更新日期：2008-10-01 00:00:00

abstract：:It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...

journal_title：Current opinion in biotechnology

authors： Kuwajima K

更新日期：1992-10-01 00:00:00

abstract：:New methods and applications in the separation of biomolecules are reviewed, with an emphasis on the large-scale recovery of proteins. Highlights include the advent of flow-through particles in perfusion chromatography, which allows for very high flow rates, while retaining a high chromatographic efficiency. ...

journal_title：Current opinion in biotechnology

authors： Glad M,Larsson PO

更新日期：1991-06-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:During the past decade, the increasing knowledge of flavonoid biosynthesis and the important function of flavonoid compounds in plants and in human and animal nutrition have made the biosynthetic pathways to flavonoids and isoflavonoids excellent targets for metabolic engineering. Recent strategies have included intro...

journal_title：Current opinion in biotechnology

authors： Forkmann G,Martens S

更新日期：2001-04-01 00:00:00

abstract：:The world continues to increase its energy use, brought about by an expanding population and a desire for a greater standard of living. This energy use coupled with the realization of the impact of carbon dioxide on the climate, has led us to reanalyze the potential of plant-based biofuels. Of the potential sources of...

journal_title：Current opinion in biotechnology

authors： Jones CS,Mayfield SP

更新日期：2012-06-01 00:00:00

abstract：:Oil prices and government mandates have catalyzed rapid growth of nonfossil transportation fuels in recent years, with a large focus on ethanol from energy crops, but the food crops used as first-generation energy crops today are not optimized for this purpose. We show that the theoretical efficiency of conversion of ...

journal_title：Current opinion in biotechnology

authors： Heaton EA,Flavell RB,Mascia PN,Thomas SR,Dohleman FG,Long SP

更新日期：2008-06-01 00:00:00

abstract：:Novel biotechnological approaches such as Metabolic Engineering (ME) and New Plant Breeding Techniques (NPBTs) are currently being developed to produce biological compounds for food and non-food products. NPBTs span a range of methods for in vivo production in crops, some of which are classified as GMOs while others a...

journal_title：Current opinion in biotechnology

authors： Pei L,Schmidt M

更新日期：2019-04-01 00:00:00

abstract：:Transgenic experimentation has become a crucial part of hypertension and atherosclerosis research, and is growing more important in several other areas of cardiovascular disease. It has recently made a particular contribution to understanding the role of the renin-angiotensin system in controlling hypertension. The st...

journal_title：Current opinion in biotechnology

authors： Barrett G,Mullins JJ

更新日期：1992-12-01 00:00:00

abstract：:The structure of microbial communities is key to their functionality. However, this structure is likely to be influenced by adaptive genetic change in members of the community, which can occur over a matter of days. Changes in community structure can in turn influence the evolutionary trajectories of species within th...

journal_title：Current opinion in biotechnology

authors： O'Brien S,Hodgson DJ,Buckling A

更新日期：2013-08-01 00:00:00

abstract：:Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...

journal_title：Current opinion in biotechnology

authors： Sittinger M,Hutmacher DW,Risbud MV

更新日期：2004-10-01 00:00:00

abstract：:Fungal ligninolytic enzymes have broad biotechnological applications. Particularly laccases and certain fungal class II peroxidases from white-rot basidiomycetes are considered in degradation of persistent organic pollutants. Promising processes with reusable immobilized laccases in special reactors have been develope...

journal_title：Current opinion in biotechnology

authors： Kües U

更新日期：2015-06-01 00:00:00

abstract：:Broad application of microbial fuel cells will require substantial increases in current density. A better understanding of the microbiology of these systems may help. Recent studies have greatly expanded the range of microorganisms known to function either as electrode-reducing microorganisms at the anode or as electr...

journal_title：Current opinion in biotechnology

authors： Lovley DR

更新日期：2008-12-01 00:00:00

abstract：:Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...

journal_title：Current opinion in biotechnology

authors： Vanholme B,El Houari I,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:During 1993, significant advances have been achieved in applications of DNA analysis to forensic science, disease assessment, and animal/plant identification. These advances include the development of simple and high sample-throughput techniques for highly informative personal identification, rapid screening for patho...

journal_title：Current opinion in biotechnology

authors： Alford RL,Caskey CT

更新日期：1994-02-01 00:00:00

abstract：:The gastrointestinal (GI) tract is a complex system characterized by multiple cell types with a determined architectural arrangement. Tissue engineering of the GI tract aims to reinstate the architecture and function of all structural layers. The key point for successful tissue regeneration includes the use of cells/b...

journal_title：Current opinion in biotechnology

authors： Bitar KN,Zakhem E

更新日期：2013-10-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing to predict the expr...

journal_title：Current opinion in biotechnology

authors： Wirth D,Gama-Norton L,Riemer P,Sandhu U,Schucht R,Hauser H

更新日期：2007-10-01 00:00:00

abstract：:The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet ke...

journal_title：Current opinion in biotechnology

authors： Parinov S,Sundaresan V

更新日期：2000-04-01 00:00:00

abstract：:The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medi...

journal_title：Current opinion in biotechnology

authors： Schwechheimer SK,Becker J,Wittmann C

更新日期：2018-12-01 00:00:00