Current strategies for cell delivery in cartilage and bone regeneration.


:Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, improved tissue differentiation, suitable handling and properties for delivery and fixation into patients. Delivery of cells can be achieved using injectable matrices, soft scaffolds, membranes, solid load-bearing scaffolds or immunoprotective macroencapsulation. Thus, to expand the clinical potential, next generation therapies will depend on smart delivery concepts that make use of the regenerative potential of stem cells, morphogenetic growth factors and biomimetic materials.


Curr Opin Biotechnol


Sittinger M,Hutmacher DW,Risbud MV




Has Abstract


2004-10-01 00:00:00














  • Directed evolution and synthetic biology applications to microbial systems.

    abstract::Biotechnology applications require engineering complex multi-genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole bio...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bassalo MC,Liu R,Gill RT

    更新日期:2016-06-01 00:00:00

  • Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture.

    abstract::The increasing demand for a steady, healthy food supply requires an efficient control of the major pests and plant diseases. Current management practices are based largely on the application of synthetic pesticides. The excessive use of agrochemicals has caused serious environmental and health problems. Therefore, the...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pérez-García A,Romero D,de Vicente A

    更新日期:2011-04-01 00:00:00

  • Impact of microbial transformation of food on health - from fermented foods to fermentation in the gastro-intestinal tract.

    abstract::Fermentation of food components by microbes occurs both during certain food production processes and in the gastro-intestinal tract. In these processes specific compounds are produced that originate from either biotransformation reactions or biosynthesis, and that can affect the health of the consumer. In this review,...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: van Hylckama Vlieg JE,Veiga P,Zhang C,Derrien M,Zhao L

    更新日期:2011-04-01 00:00:00

  • Bacterial persistence from a system-level perspective.

    abstract::In recent years, our understanding about bacterial persistence has significantly advanced: we comprehend the persister phenotype better, more triggers for persistence entry have been found, and more insights in the involvement and role of toxin-antitoxin systems and other molecular mechanisms have been unravelled. In ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Radzikowski JL,Schramke H,Heinemann M

    更新日期:2017-08-01 00:00:00

  • Oxygen-tolerant hydrogenases in hydrogen-based technologies.

    abstract::To develop a viable H2 technology, production of H2 has to be significantly enlarged by using renewable resources. One option of generating H2 is the photosynthetic conversion of sunlight and water directly to H2 and O2. Photosystems and hydrogenases are currently being exploited for the design of efficient H2-produci...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Friedrich B,Fritsch J,Lenz O

    更新日期:2011-06-01 00:00:00

  • Technoeconomic analysis for biofuels and bioproducts.

    abstract::Technoeconomic analysis (TEA) is an approach for conducting process design and simulation, informed by empirical data, to estimate capital costs, operating costs, mass balances, and energy balances for a commercial scale biorefinery. TEA serves as a useful method to screen potential research priorities, identify cost ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Scown CD,Baral NR,Yang M,Vora N,Huntington T

    更新日期:2021-01-18 00:00:00

  • Combined metal catalysis and biocatalysis for an efficient deracemization process.

    abstract::Driven by the demand to enhance the economic balance of chemical processes, the transformation of a racemate into a single stereoisomer (the deracemization process) is one of the most important areas of research. The recent development of the combined use of enzyme and metal catalysis has led to efficient deracemizati...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pàmies O,Bäckvall JE

    更新日期:2003-08-01 00:00:00

  • Logic of gene regulatory networks.

    abstract::Regulatory networks of transcription factors and signaling molecules lie at the heart of development. Their architecture implements logic functions whose execution propels cells from one regulatory state to the next, thus driving development forward. As an example of a subcircuit that translates transcriptional input ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Materna SC,Davidson EH

    更新日期:2007-08-01 00:00:00

  • Molecular tools for pathway engineering in Saccharomyces cerevisiae.

    abstract::Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Besada-Lombana PB,McTaggart TL,Da Silva NA

    更新日期:2018-10-01 00:00:00

  • Stable isotope probing of carbon flow in the plant holobiont.

    abstract::Microbial communities associated with a plant host, constituting a holobiont, affect the physiology and growth of the plant via metabolites that are mainly derived from their photosynthates. The structure and function of active microbial communities that assimilate root exudates can be tracked by using stable isotope ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Haichar FEZ,Heulin T,Guyonnet JP,Achouak W

    更新日期:2016-10-01 00:00:00

  • Novel methods for cloning and engineering genes using the polymerase chain reaction.

    abstract::Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rashtchian A

    更新日期:1995-02-01 00:00:00

  • Trade-offs between gene expression, growth and phenotypic diversity in microbial populations.

    abstract::Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

    更新日期:2020-04-01 00:00:00

  • Continuous integrated manufacturing of therapeutic proteins.

    abstract::With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Karst DJ,Steinebach F,Morbidelli M

    更新日期:2018-10-01 00:00:00

  • Fungal enzymes for environmental management.

    abstract::Fungal ligninolytic enzymes have broad biotechnological applications. Particularly laccases and certain fungal class II peroxidases from white-rot basidiomycetes are considered in degradation of persistent organic pollutants. Promising processes with reusable immobilized laccases in special reactors have been develope...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kües U

    更新日期:2015-06-01 00:00:00

  • Nanotechnology: convergence with modern biology and medicine.

    abstract::The worldwide emergence of nanoscale science and engineering was marked by the announcement of the National Nanotechnology Initiative (NNI) in January 2000. Recent research on biosystems at the nanoscale has created one of the most dynamic science and technology domains at the confluence of physical sciences, molecula...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Roco MC

    更新日期:2003-06-01 00:00:00

  • Systems biology as a foundation for genome-scale synthetic biology.

    abstract::As the ambitions of synthetic biology approach genome-scale engineering, comprehensive characterization of cellular systems is required, as well as a means to accurately model cell-scale molecular interactions. These requirements are coincident with the goals of systems biology and, thus, systems biology will become t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Barrett CL,Kim TY,Kim HU,Palsson BØ,Lee SY

    更新日期:2006-10-01 00:00:00

  • Shared mechanisms among probiotic taxa: implications for general probiotic claims.

    abstract::Strain-specificity of probiotic effects has been a cornerstone principle of probiotic science for decades. Certainly, some important mechanisms are present in only a few probiotic strains. But scientific advances now reveal commonalities among members of certain taxonomic groups of probiotic microbes. Some clinical be...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Sanders ME,Benson A,Lebeer S,Merenstein DJ,Klaenhammer TR

    更新日期:2018-02-01 00:00:00

  • Origin and analysis of microbial population heterogeneity in bioprocesses.

    abstract::Heterogeneity of industrial production cultures is accepted to a certain degree; however, the underlying mechanisms are seldom perceived or included in the development of new bioprocess control strategies. Population heterogeneity and its basics, perceptible in the diverse proficiency of cells, begins with asymmetric ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Müller S,Harms H,Bley T

    更新日期:2010-02-01 00:00:00

  • Rational recognition of nucleic acid sequences.

    abstract::Recent progress in synthetic and computational chemistry has made it possible to develop certain novel drug candidates. Drug candidates for genetic diseases, such as cancer, may also be designed on the basis of structural information obtained using X-ray analysis and NMR, as well as evidence from biological techniques...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: lida H,Jia G,Lown JW

    更新日期:1999-02-01 00:00:00

  • Protein fragment complementation strategies for biochemical network mapping.

    abstract::The organization of biochemical networks that make up the living cell can be defined by studying the dynamics of protein-protein interactions. To this end, experimental strategies based on protein fragment complementation assays (PCAs) have been used to map biochemical networks and to identify novel components of thes...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Michnick SW

    更新日期:2003-12-01 00:00:00

  • Ribozymes.

    abstract::RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rossi JJ

    更新日期:1992-02-01 00:00:00

  • Bacteriophage ecology in environmental biotechnology processes.

    abstract::Heterotrophic bacteria are an integral part of any environmental biotechnology process (EBP). Therefore, factors controlling bacterial abundance, activity, and community composition are central to the understanding of such processes. Among these factors, top-down control by bacteriophage predation has so far received ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Shapiro OH,Kushmaro A

    更新日期:2011-06-01 00:00:00

  • Genetics of plant-pathogen interactions

    abstract::Progress has occurred in understanding the function of disease-resistance genes that govern the resistance of plants to pathogens, and pathogen-produced molecules, called elicitors, that resistance genes key on. Data support the elicitor-receptor model wherein resistant plants contain receptors for pathogen elicitors....

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Ji C,Smith-Becker J,Keen NT

    更新日期:1998-04-01 00:00:00

  • Strategies for extended serum half-life of protein therapeutics.

    abstract::With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kontermann RE

    更新日期:2011-12-01 00:00:00

  • Visualizing life with ambient mass spectrometry.

    abstract::Since the development of desorption electrospray ionization (DESI), many other ionization methods for ambient and atmospheric pressure mass spectrometry have been developed. Ambient ionization mass spectrometry has now been used for a wide variety of biological applications, including plant science, microbiology, neur...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hsu CC,Dorrestein PC

    更新日期:2015-02-01 00:00:00

  • The role of sigmaB in the stress response of Gram-positive bacteria -- targets for food preservation and safety.

    abstract::The alternative sigma factor sigmaB modulates the stress response of several Gram-positive bacteria, including Bacillus subtilis and the food-borne human pathogens Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus. In all these bacteria, sigmaB is responsible for the transcription of genes that can con...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: van Schaik W,Abee T

    更新日期:2005-04-01 00:00:00

  • Iron biofortification in the 21st century: setting realistic targets, overcoming obstacles, and new strategies for healthy nutrition.

    abstract::Plant-based foods offer a wide range of nutrients that are essential for human and animal health. Among these nutrients, iron stands out as one of the most important micronutrients. Increasing the iron content in many staple and non-staple plant foods continues to be a goal of many scientists around the world. However...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Vasconcelos MW,Gruissem W,Bhullar NK

    更新日期:2017-04-01 00:00:00

  • Engineering yeast for utilization of alternative feedstocks.

    abstract::Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xyl...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Yaguchi A,Spagnuolo M,Blenner M

    更新日期:2018-10-01 00:00:00

  • Detergent proteases.

    abstract::Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes. The number of molecules identified and characterized, however, is in clear opposition to the number of molecules that are ente...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Maurer KH

    更新日期:2004-08-01 00:00:00

  • Genetic alphabet expansion biotechnology by creating unnatural base pairs.

    abstract::Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UB...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lee KH,Hamashima K,Kimoto M,Hirao I

    更新日期:2018-06-01 00:00:00