Directed evolution and synthetic biology applications to microbial systems.

Abstract:

:Biotechnology applications require engineering complex multi-genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole biological systems toward desired phenotypes without requiring prior knowledge of the genetic basis of the targeted trait. Recent developments in the synthetic biology field accelerates the directed evolution cycle, facilitating engineering of increasingly complex traits in biological systems. In this review, we summarize some of the most recent advances in directed evolution and synthetic biology that allows engineering of complex traits in microbial systems. Then, we discuss applications that can be achieved through engineering at the systems level.

journal_name

Curr Opin Biotechnol

authors

Bassalo MC,Liu R,Gill RT

doi

10.1016/j.copbio.2016.03.016

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

126-133

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(16)30072-6

journal_volume

39

pub_type

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