Customised nucleic acid libraries for enhanced aptamer selection and performance.

Abstract:

:Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applications. However, the variety of possible interactions with a putative target molecule is limited by the chemical repertoire of the natural nucleobases. Consequently, many desired targets are not addressable by aptamers. This obstacle is overcome by broadening the chemical diversity of aptamers, mainly achieved by nucleobase-modifications and the introduction of novel bases or base pairs. We discuss these achievements and the characteristics of the respective modified aptamers, reflected by SOMAmers (slow off-rate modified aptamers), clickmers, and aptamers bearing an expanded genetic alphabet.

journal_name

Curr Opin Biotechnol

authors

Pfeiffer F,Rosenthal M,Siegl J,Ewers J,Mayer G

doi

10.1016/j.copbio.2017.03.026

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

111-118

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(17)30050-2

journal_volume

48

pub_type

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