Abstract:
:Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applications. However, the variety of possible interactions with a putative target molecule is limited by the chemical repertoire of the natural nucleobases. Consequently, many desired targets are not addressable by aptamers. This obstacle is overcome by broadening the chemical diversity of aptamers, mainly achieved by nucleobase-modifications and the introduction of novel bases or base pairs. We discuss these achievements and the characteristics of the respective modified aptamers, reflected by SOMAmers (slow off-rate modified aptamers), clickmers, and aptamers bearing an expanded genetic alphabet.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Pfeiffer F,Rosenthal M,Siegl J,Ewers J,Mayer Gdoi
10.1016/j.copbio.2017.03.026subject
Has Abstractpub_date
2017-12-01 00:00:00pages
111-118eissn
0958-1669issn
1879-0429pii
S0958-1669(17)30050-2journal_volume
48pub_type
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