Abstract:
:The intestinal microbiota plays a profound role in human health and extensive research has been dedicated to identify microbiota aberrations that are associated with disease. Most of this work has been targeting the large intestine and fecal microbiota, while the small intestine microbiota may also have a profound impact on various aspects of the host's physiology, including immune, metabolic and endocrine functions. This review highlights the recent advances made in the study of the human small intestine microbiota. In addition, it describes recent human and animal studies that underpin the importance of this part of the intestine for health of the host organism.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
El Aidy S,van den Bogert B,Kleerebezem Mdoi
10.1016/j.copbio.2014.09.005subject
Has Abstractpub_date
2015-04-01 00:00:00pages
14-20eissn
0958-1669issn
1879-0429pii
S0958-1669(14)00165-7journal_volume
32pub_type
杂志文章,评审abstract::In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(98)80037-2
更新日期:1998-10-01 00:00:00
abstract::The concept of whole genome amplification is something that has arisen in the past few years as the polymerase chain reaction (PCR) has been adapted to replicate regions of genomes that are of biological interest. The applications are many--forensic science, embryonic disease diagnosis, bioterrorism genome detection, ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(02)00286-0
更新日期:2002-02-01 00:00:00
abstract::Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1-200 microm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit inc...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0958-1669(99)80060-3
更新日期:1999-08-01 00:00:00
abstract::Microorganisms play important roles in the environmental fate of toxic metals and radionuclides with a multiplicity of mechanisms effecting transformations between soluble and insoluble forms. These mechanisms are integral components of natural biogeochemical cycles and are of potential for both in situ and ex situ bi...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(00)00095-1
更新日期:2000-06-01 00:00:00
abstract::The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2012.08.011
更新日期:2013-06-01 00:00:00
abstract::During the last years, a lot of efforts have been put into developing novel sensors and biosensors systems for monitoring the occurrence of emerging pollutants in the environment. These analyses are often challenging because of the variety of physico-chemical properties of these substances, the extremely diluted conce...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.02.017
更新日期:2017-06-01 00:00:00
abstract::Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connec...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2016.03.018
更新日期:2016-10-01 00:00:00
abstract::Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(02)00380-4
更新日期:2002-12-01 00:00:00
abstract::Academic microfluidics has decisively shifted in recent years from the research on phenomenology and proof-of-concept fluidic functionalities to the developments oriented at applications with biology, medicine and biotechnology in prime focus. Significant efforts are made to demonstrate that microfluidics can be used ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2018.08.004
更新日期:2019-02-01 00:00:00
abstract::The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(03)00060-0
更新日期:2003-06-01 00:00:00
abstract::Fifteen years have passed since T-cell receptor (TCR) genes were identified (reviewed in [1]). Unlike the situation for antibodies, no direct structural information on the TCR proteins has been available for most of this time. Recently, however, the crystal structures of isolated alpha and beta chains were determined,...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(98)80004-9
更新日期:1998-08-01 00:00:00
abstract::Antibacterial strategies targeting bacterial adhesion to substrates are considered a valuable alternative to traditional antibiotic therapy, in view of the great advantage they bring in combating the infectious process at the very early stage without selecting for drug resistant cells. Amongst bioactive compounds with...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2011.08.006
更新日期:2012-04-01 00:00:00
abstract::Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2012.10.020
更新日期:2013-06-01 00:00:00
abstract::In recent years, our understanding about bacterial persistence has significantly advanced: we comprehend the persister phenotype better, more triggers for persistence entry have been found, and more insights in the involvement and role of toxin-antitoxin systems and other molecular mechanisms have been unravelled. In ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.02.012
更新日期:2017-08-01 00:00:00
abstract::Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2017.12.002
更新日期:2018-10-01 00:00:00
abstract::Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2019.08.004
更新日期:2020-04-01 00:00:00
abstract::Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(96)80056-5
更新日期:1996-10-01 00:00:00
abstract::Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2005.07.007
更新日期:2005-10-01 00:00:00
abstract::Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactio...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2004.06.007
更新日期:2004-08-01 00:00:00
abstract::The design and construction of synthetic gene circuits with complex spatiotemporal dynamics was pioneered in bacteria, but it took almost a decade until synthetic biologists were able to construct genetic circuits with complex spatiotemporal dynamics in mammalian cells. This review highlights the most recent advances ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2010.07.006
更新日期:2010-10-01 00:00:00
abstract::Novel biotechnological approaches such as Metabolic Engineering (ME) and New Plant Breeding Techniques (NPBTs) are currently being developed to produce biological compounds for food and non-food products. NPBTs span a range of methods for in vivo production in crops, some of which are classified as GMOs while others a...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2018.08.012
更新日期:2019-04-01 00:00:00
abstract::The biosynthesis of the solvents 1-butanol and acetone is restricted to species of the genus Clostridium, a diverse group of Gram-positive, endospore forming anaerobes comprising toxin-producing strains as well as terrestrial non-pathogenic species of biotechnological impact. Among solventogenic clostridia, Clostridiu...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2011.01.011
更新日期:2011-10-01 00:00:00
abstract::Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites b...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章
doi:10.1016/j.copbio.2010.01.012
更新日期:2010-02-01 00:00:00
abstract::Originally proposed one decade ago, the idea of engineering proteins outside the immunoglobulin family for novel binding functions has evolved as a powerful technology. Several classes of protein scaffolds proved to yield reagents with specificities and affinities in a range that was previously considered unique to an...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2007.04.010
更新日期:2007-08-01 00:00:00
abstract::Plant-based foods offer a wide range of nutrients that are essential for human and animal health. Among these nutrients, iron stands out as one of the most important micronutrients. Increasing the iron content in many staple and non-staple plant foods continues to be a goal of many scientists around the world. However...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2016.10.001
更新日期:2017-04-01 00:00:00
abstract::Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage. Given this situation, one would predict translational problems with an abundant...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0958-1669(95)80082-4
更新日期:1995-10-01 00:00:00
abstract::The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet ke...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(00)00075-6
更新日期:2000-04-01 00:00:00
abstract::Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the p...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(00)00116-6
更新日期:2000-08-01 00:00:00
abstract::To develop a viable H2 technology, production of H2 has to be significantly enlarged by using renewable resources. One option of generating H2 is the photosynthetic conversion of sunlight and water directly to H2 and O2. Photosystems and hydrogenases are currently being exploited for the design of efficient H2-produci...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章
doi:10.1016/j.copbio.2011.01.006
更新日期:2011-06-01 00:00:00
abstract::Estrogen receptors are multi-domain proteins that interact with other proteins and DNA to fulfil their function: the regulation of transcription. During the past 2-3 years, our understanding of this complex process has increased tremendously as crystal structures of isolated ligand-binding domains in complex with vari...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0958-1669(99)00034-8
更新日期:1999-12-01 00:00:00