Alternative bioseparation operations: life beyond packed-bed chromatography.

Abstract:

:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attractive proposition, even if only a reduction in the extent of use of packed beds can be realized. This paper reviews the current state of unit operations posing as chromatography alternatives--including membrane filtration, aqueous two-phase extraction, three-phase partitioning, precipitation, crystallization, monoliths and membrane chromatography--and their potential to do the unthinkable.

journal_name

Curr Opin Biotechnol

authors

Przybycien TM,Pujar NS,Steele LM

doi

10.1016/j.copbio.2004.08.008

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

469-78

issue

5

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(04)00114-4

journal_volume

15

pub_type

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