Nanophotonic label-free biosensors for environmental monitoring.

abstract：:The structure of microbial communities is key to their functionality. However, this structure is likely to be influenced by adaptive genetic change in members of the community, which can occur over a matter of days. Changes in community structure can in turn influence the evolutionary trajectories of species within th...

journal_title：Current opinion in biotechnology

authors： O'Brien S,Hodgson DJ,Buckling A

更新日期：2013-08-01 00:00:00

abstract：:Biomaterials are increasingly being developed as in vitro microenvironments mimicking in vivo stem cell niches. However, current macroscale methodologies to produce these niche models fail to recapitulate the spatial and temporal characteristics of the complex native stem cell regulatory systems. Microfluidic technolo...

journal_title：Current opinion in biotechnology

authors： Kobel S,Lutolf MP

更新日期：2011-10-01 00:00:00

abstract：:Transgenic experimentation has become a crucial part of hypertension and atherosclerosis research, and is growing more important in several other areas of cardiovascular disease. It has recently made a particular contribution to understanding the role of the renin-angiotensin system in controlling hypertension. The st...

journal_title：Current opinion in biotechnology

authors： Barrett G,Mullins JJ

更新日期：1992-12-01 00:00:00

abstract：:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

journal_title：Current opinion in biotechnology

authors： Rashtchian A

更新日期：1995-02-01 00:00:00

abstract：:Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent...

journal_title：Current opinion in biotechnology

authors： Stoloff DH,Wanunu M

更新日期：2013-08-01 00:00:00

abstract：:Contamination of the vadose zone with various pollutants is a world-wide problem, and often technical or economic constraints impose remediation without excavation. In situ bioremediation in the vadose zone by bioventing has become a standard remediation technology for light spilled petroleum products. In this review,...

journal_title：Current opinion in biotechnology

authors： Höhener P,Ponsin V

更新日期：2014-06-01 00:00:00

abstract：:In recent years a number of aglycosylated therapeutic antibodies have entered the clinic. The clinical evaluation of these antibodies has served to dispel concerns that the absence of the ubiquitous N297 glycan in the Fc of IgG might result in immunogenicity, poor in vivo stability or unfavorable pharmacokinetics. Imp...

journal_title：Current opinion in biotechnology

authors： Jung ST,Kang TH,Kelton W,Georgiou G

更新日期：2011-12-01 00:00:00

abstract：:Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in severa...

journal_title：Current opinion in biotechnology

authors： Krishnan M,Namasivayam V,Lin R,Pal R,Burns MA

更新日期：2001-02-01 00:00:00

abstract：:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for preci...

journal_title：Current opinion in biotechnology

authors： Tolle F,Stücheli P,Fussenegger M

更新日期：2019-10-01 00:00:00

abstract：:Fifteen years have passed since T-cell receptor (TCR) genes were identified (reviewed in [1]). Unlike the situation for antibodies, no direct structural information on the TCR proteins has been available for most of this time. Recently, however, the crystal structures of isolated alpha and beta chains were determined,...

journal_title：Current opinion in biotechnology

authors： Garcia KC,Teyton L

更新日期：1998-08-01 00:00:00

abstract：:Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology can also ...

journal_title：Current opinion in biotechnology

authors： Olins PO,Lee SC

更新日期：1993-10-01 00:00:00

abstract：:Vernalization is the process by which flowering is promoted by prolonged exposure to the cold of a typical winter. In certain plant species, the role of vernalization is to suppress the expression of genes that encode repressors of flowering. In Arabidopsis, this suppression is an epigenetic phenomenon in the sense th...

journal_title：Current opinion in biotechnology

authors： Amasino RM

更新日期：2005-04-01 00:00:00

abstract：:Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated biochemical reactions by in vitro assembling a number of enzymes or their complexes and coenzymes. Assembly of numerous enzymes without cellular membrane, gene regulation, or undesired pathway can circumvent some of the obstacle...

journal_title：Current opinion in biotechnology

authors： Zhang YH,Sun J,Zhong JJ

更新日期：2010-10-01 00:00:00

abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...

journal_title：Current opinion in biotechnology

authors： O'Malley J,Woltjen K,Kaji K

更新日期：2009-10-01 00:00:00

abstract：:The use of novel materials in the nano-scale size range for applications in devices, drugs and diagnostic agents comes with a number of new opportunities, and also serious challenges to human applications. The larger size of particulate-based agents, as compared to traditional drugs, allows for the significant advanta...

journal_title：Current opinion in biotechnology

authors： Scheinberg DA,Grimm J,Heller DA,Stater EP,Bradbury M,McDevitt MR

更新日期：2017-08-01 00:00:00

abstract：:Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...

journal_title：Current opinion in biotechnology

authors： DelRosso NV,Derr ND

更新日期：2017-08-01 00:00:00

abstract：:Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

journal_title：Current opinion in biotechnology

authors： Junker BH

更新日期：2014-04-01 00:00:00

abstract：:Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage. Given this situation, one would predict translational problems with an abundant...

journal_title：Current opinion in biotechnology

authors： Kane JF

更新日期：1995-10-01 00:00:00

abstract：:The concept of whole genome amplification is something that has arisen in the past few years as the polymerase chain reaction (PCR) has been adapted to replicate regions of genomes that are of biological interest. The applications are many--forensic science, embryonic disease diagnosis, bioterrorism genome detection, ...

journal_title：Current opinion in biotechnology

authors： Hawkins TL,Detter JC,Richardson PM

更新日期：2002-02-01 00:00:00

abstract：:Adverse environmental conditions pose serious limitations to agricultural production. Classical biotechnological approaches towards increasing abiotic stress tolerance focus on boosting plant endogenous defence mechanisms. However, overexpression of regulatory elements or effectors is usually accompanied by growth han...

journal_title：Current opinion in biotechnology

authors： Cabello JV,Lodeyro AF,Zurbriggen MD

更新日期：2014-04-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:Recent advances in miniaturization and automation of technologies have enabled cell-based assay high-throughput screening, bringing along new challenges in data analysis. Automation, standardization, reproducibility have become requirements for qualitative research. The Bioconductor community has worked in that direct...

journal_title：Current opinion in biotechnology

authors： Le Meur N

更新日期：2013-02-01 00:00:00

abstract：:Despite their central role in directing some of the most complex biological processes, carbohydrates--nature's other information carrying biopolymer--have been largely ignored as building blocks for synthetic hierarchical assemblies. The non-stoichiometric binding and astronomical diversity characteristic of carbohydr...

journal_title：Current opinion in biotechnology

authors： Han X,Zheng Y,Munro CJ,Ji Y,Braunschweig AB

更新日期：2015-08-01 00:00:00

abstract：:Metabolic engineering is not another form of classic manipulation of intermediary metabolism; rather, it is the purposeful design of metabolic networks. The metabolic engineering approach examines biochemical reactions in their entirety, rather than individually, and is concerned with the construction of novel pathway...

journal_title：Current opinion in biotechnology

authors： Stephanopoulos G

更新日期：1994-02-01 00:00:00

abstract：:Heterogeneity of industrial production cultures is accepted to a certain degree; however, the underlying mechanisms are seldom perceived or included in the development of new bioprocess control strategies. Population heterogeneity and its basics, perceptible in the diverse proficiency of cells, begins with asymmetric ...

journal_title：Current opinion in biotechnology

authors： Müller S,Harms H,Bley T

更新日期：2010-02-01 00:00:00

abstract：:Recent progress in synthetic and computational chemistry has made it possible to develop certain novel drug candidates. Drug candidates for genetic diseases, such as cancer, may also be designed on the basis of structural information obtained using X-ray analysis and NMR, as well as evidence from biological techniques...

journal_title：Current opinion in biotechnology

authors： lida H,Jia G,Lown JW

更新日期：1999-02-01 00:00:00

abstract：:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...

journal_title：Current opinion in biotechnology

authors： Olson DG,Sparling R,Lynd LR

更新日期：2015-06-01 00:00:00

abstract：:Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...

journal_title：Current opinion in biotechnology

authors： Hazen TC,Rocha AM,Techtmann SM

更新日期：2013-06-01 00:00:00

abstract：:Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xyl...

journal_title：Current opinion in biotechnology

authors： Yaguchi A,Spagnuolo M,Blenner M

更新日期：2018-10-01 00:00:00

abstract：:With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

journal_title：Current opinion in biotechnology

authors： Kontermann RE

更新日期：2011-12-01 00:00:00