Abstract：
:Recent progress in synthetic and computational chemistry has made it possible to develop certain novel drug candidates. Drug candidates for genetic diseases, such as cancer, may also be designed on the basis of structural information obtained using X-ray analysis and NMR, as well as evidence from biological techniques applied to natural products - DNA (or RNA) complexes and conjugates. The resulting designed drug candidates exhibit promising performance based on the recognition on nucleic acid sequences.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
lida H,Jia G,Lown JWdoi
10.1016/s0958-1669(99)80006-8subject
Has Abstractpub_date
1999-02-01 00:00:00pages
29-33issue
1eissn
0958-1669issn
1879-0429pii
S0958166999800068journal_volume
10pub_type
杂志文章,评审abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(96)80040-1
更新日期：1996-06-01 00:00:00
abstract：:Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destru...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(95)80069-7
更新日期：1995-08-01 00:00:00
abstract：:The use of novel materials in the nano-scale size range for applications in devices, drugs and diagnostic agents comes with a number of new opportunities, and also serious challenges to human applications. The larger size of particulate-based agents, as compared to traditional drugs, allows for the significant advanta...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2017.01.002
更新日期：2017-08-01 00:00:00
abstract：:Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2008.06.005
更新日期：2008-08-01 00:00:00
abstract：:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2017.02.011
更新日期：2017-08-01 00:00:00
abstract：:Over the review period, a significant amount of literature has been published documenting the impact of biofilms on engineered and biomedical systems. Reactor systems and analytical techniques have evolved to study the molecular chemistry and microbial ecology within biofilm layers only tens of micrometers thick, and ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(93)90125-g
更新日期：1993-04-01 00:00:00
abstract：:Is structure, rather than sequence, the key to the successful generation of truly novel proteins? While protein evolution by homologous recombination has become an established tool to explore confined regions in sequence space, the generation of functional hybrid proteins by homology-independent methods further expand...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00106-3
更新日期：2000-08-01 00:00:00
abstract：:Recent advancements in high-throughput large-scale analytical methods to sequence genomes of organisms, and to quantify gene expression, proteins, lipids and metabolites have changed the paradigm of metabolic modeling. The cost of data generation and analysis has decreased significantly, which has allowed exponential ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2015.10.010
更新日期：2016-02-01 00:00:00
abstract：:To mitigate some of the potentially deleterious environmental and agricultural consequences associated with current land-based-biofuel feedstocks, we propose the use of biofuels derived from aquatic microbial oxygenic photoautotrophs (AMOPs), more commonly known as cyanobacteria, algae, and diatoms. Herein we review t...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2008.05.007
更新日期：2008-06-01 00:00:00
abstract：:Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2006.02.001
更新日期：2006-04-01 00:00:00
abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.06.019
更新日期：2014-12-01 00:00:00
abstract：:Microbial communities are essential for most ecosystem processes and interact in highly complex ways with virtually all eukaryotes. Thus, a detailed understanding of the function of such communities is a fundamental prerequisite for microbial ecologists, applied microbiologists and microbiome researchers. Using single...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.04.018
更新日期：2016-10-01 00:00:00
abstract：:The spatiotemporal patterns of ion and metabolite levels in living cells are important in understanding signal transduction and metabolite flux. Imaging approaches using genetically encoded sensors are ideal for detecting such molecule dynamics, which are hard to capture otherwise. Recent years have seen iterative imp...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/j.copbio.2010.01.009
更新日期：2010-02-01 00:00:00
abstract：:Escherichia coli is still a very popular host for the production of recombinant proteins at an analytical or industrial scale. Secretion of the proteins into the culture medium or display at the cell surface would be preferred in many applications but is hampered by the complex two-layered cell envelope. The autotrans...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2010.07.009
更新日期：2010-10-01 00:00:00
abstract：:Wound healing can no longer be considered a generic term: it is the summation of a complex series of processes beginning with coagulation and ending in remodelling. This review, therefore, summarizes the past year's advances in growth factor research according to the roles of the individual growth factors in the speci...
journal_title：Current opinion in biotechnology
pub_type： 临床试验,杂志文章,评审
doi：10.1016/s0958-1669(05)80122-3
更新日期：1991-12-01 00:00:00
abstract：:Mismatch-repair systems have been identified in organisms ranging from Escherichia coli to humans. They can repair almost all DNA base pair mismatches as well as small insertion/deletion mismatches. Molecular and biochemical analyses have shown that the core components of eukaryotic mismatch-repair systems are highly ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(94)90079-5
更新日期：1994-12-01 00:00:00
abstract：:Fundamental biological processes such as cell-cell communication, signal transduction, molecular transport and energy conversion are performed by membrane proteins. These important proteins are studied best in their native environment, the lipid bilayer. The atomic force microscope (AFM) is the instrument of choice to...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.11.032
更新日期：2012-08-01 00:00:00
abstract：:The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medi...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2018.07.001
更新日期：2018-12-01 00:00:00
abstract：:Drought, high salinity and low temperature are major environmental factors that limit plant productivity. Plants respond and adapt to these stresses in order to survive. Signaling pathways are induced in response to environmental stress and recent molecular and genetic studies have revealed that these pathways involve...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(03)00030-2
更新日期：2003-04-01 00:00:00
abstract：:Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2003.12.002
更新日期：2004-02-01 00:00:00
abstract：:Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology can also ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(93)90071-4
更新日期：1993-10-01 00:00:00
abstract：:The recent technological advances in next generation sequencing have brought the field closer to the goal of reconstructing all genomes within a community by presenting high throughput sequencing at much lower costs. While these next-generation sequencing technologies have allowed a massive increase in available raw s...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.11.013
更新日期：2012-02-01 00:00:00
abstract：:Antisense technology is finding increasing application not only in clinical development, but also for cellular engineering. Several types of antisense methods (e.g. antisense oligonucleotides, antisense RNA and small interfering RNA) can be used to inhibit the expression of a target gene. These antisense methods are b...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(03)00115-0
更新日期：2003-10-01 00:00:00
abstract：:Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2005.12.003
更新日期：2006-02-01 00:00:00
abstract：:Although metabolic networks can be readily reconstructed through comparative genomics, the reconstruction of regulatory networks has been hindered by the relatively low level of evolutionary conservation of their molecular components. Recent developments in experimental techniques have allowed the generation of vast a...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2003.11.002
更新日期：2004-02-01 00:00:00
abstract：:Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connec...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.03.018
更新日期：2016-10-01 00:00:00
abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2009.09.005
更新日期：2009-10-01 00:00:00
abstract：:Progress has occurred in understanding the function of disease-resistance genes that govern the resistance of plants to pathogens, and pathogen-produced molecules, called elicitors, that resistance genes key on. Data support the elicitor-receptor model wherein resistant plants contain receptors for pathogen elicitors....
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/s0958-1669(98)80116-x
更新日期：1998-04-01 00:00:00
abstract：:Autonomous vehicles are becoming an essential tool in a wide range of environmental applications that include ambient data acquisition, remote sensing, and mapping of the spatial extent of pollutant spills. Among these applications, pollution source localization has drawn increasing interest due to its scientific and ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2017.01.009
更新日期：2017-06-01 00:00:00
abstract：:The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.08.011
更新日期：2013-06-01 00:00:00
