Peptide ligation and semisynthesis.

abstract：:Dramatic technical progress in RNA synthesis and structure determination has allowed several difficulties inherent to the preparation, handling and structural analysis of RNA to be overcome, and this has led to a wealth of information about RNA structure and its relationship with biological function. It is now fully r...

journal_title：Current opinion in biotechnology

authors： Hermann T,Westhof E

更新日期：1998-02-01 00:00:00

abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...

journal_title：Current opinion in biotechnology

authors： Schwager J,Mohajeri MH,Fowler A,Weber P

更新日期：2008-04-01 00:00:00

abstract：:Tremendous advances in biocatalytic approaches to oligosaccharide synthesis have taken place in the past two years. The use of isolated enzymes, both glycosyltransferases and glycosidases, or engineered whole cells allows the preparation of natural oligosaccharides and analogs required for glycobiology research. ...

journal_title：Current opinion in biotechnology

authors： Palcic MM

更新日期：1999-12-01 00:00:00

abstract：:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

journal_title：Current opinion in biotechnology

authors： Eckart MR,Bussineau CM

更新日期：1996-10-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00

abstract：:In recent years a number of aglycosylated therapeutic antibodies have entered the clinic. The clinical evaluation of these antibodies has served to dispel concerns that the absence of the ubiquitous N297 glycan in the Fc of IgG might result in immunogenicity, poor in vivo stability or unfavorable pharmacokinetics. Imp...

journal_title：Current opinion in biotechnology

authors： Jung ST,Kang TH,Kelton W,Georgiou G

更新日期：2011-12-01 00:00:00

abstract：:The recent adoption of whole plasmid genome sequencing as a routine analytical technique has provided the basis for cataloging the historical events through which plasmids are assembled from the available families of modular plasmid components. Horizontal gene transfer mediated by plasmids plays an important role in t...

journal_title：Current opinion in biotechnology

authors： Dennis JJ

更新日期：2005-06-01 00:00:00

abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...

journal_title：Current opinion in biotechnology

authors： O'Malley J,Woltjen K,Kaji K

更新日期：2009-10-01 00:00:00

abstract：:Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...

journal_title：Current opinion in biotechnology

authors： Zubarev RA

更新日期：2004-02-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...

journal_title：Current opinion in biotechnology

authors： Martin CH,Kaufman DS

更新日期：2005-10-01 00:00:00

abstract：:The development of contrast agents that can be localized to a particular tissue or cellular epitope will potentially allow the noninvasive visualization and characterization of a variety of disease states. Recent advances have been made in the field of molecular imaging with magnetic resonance imaging and ultrasound a...

journal_title：Current opinion in biotechnology

authors： Morawski AM,Lanza GA,Wickline SA

更新日期：2005-02-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:Fluorescent proteins (FPs) and fluorescent protein-derived biosensors are indispensable tools in life sciences. They make it possible to probe the location, activity, or interaction of molecules of interest from a subcellular to a multicellular scale. The desire for high-resolution and multidimensional information imp...

journal_title：Current opinion in biotechnology

authors： Duwé S,Dedecker P

更新日期：2019-08-01 00:00:00

abstract：:New technologies have allowed researchers to better design, build, and analyze complex consortia. These developments are fueling a wider implementation of consortium-based bioprocessing by leveraging synthetic biology, delivering on the field's multitudinous promises of higher efficiencies, superior resiliency, augmen...

journal_title：Current opinion in biotechnology

authors： Rapp KM,Jenkins JP,Betenbaugh MJ

更新日期：2020-12-01 00:00:00

abstract：:Herbicides are economically important, but the non-point pollution that they cause may disrupt the surrounding environment. Phytoremediation of herbicides has been well studied using conventional plants. Transgenic plants produced for metabolizing herbicides and long-persisting pollutants can be used for phytoremediat...

journal_title：Current opinion in biotechnology

authors： Kawahigashi H

更新日期：2009-04-01 00:00:00

abstract：:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...

journal_title：Current opinion in biotechnology

authors： Vervoort Y,Linares AG,Roncoroni M,Liu C,Steensels J,Verstrepen KJ

更新日期：2017-08-01 00:00:00

abstract：:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...

journal_title：Current opinion in biotechnology

authors： Olson DG,Sparling R,Lynd LR

更新日期：2015-06-01 00:00:00

abstract：:The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...

journal_title：Current opinion in biotechnology

authors： Ogawa J,Shimizu S

更新日期：2002-08-01 00:00:00

abstract：:Both cyanobacteria and eukaryotic microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of...

journal_title：Current opinion in biotechnology

authors： Wijffels RH,Kruse O,Hellingwerf KJ

更新日期：2013-06-01 00:00:00

abstract：:Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage. Given this situation, one would predict translational problems with an abundant...

journal_title：Current opinion in biotechnology

authors： Kane JF

更新日期：1995-10-01 00:00:00

abstract：:Technoeconomic analysis (TEA) is an approach for conducting process design and simulation, informed by empirical data, to estimate capital costs, operating costs, mass balances, and energy balances for a commercial scale biorefinery. TEA serves as a useful method to screen potential research priorities, identify cost ...

journal_title：Current opinion in biotechnology

authors： Scown CD,Baral NR,Yang M,Vora N,Huntington T

更新日期：2021-01-18 00:00:00

abstract：:Although metabolic networks can be readily reconstructed through comparative genomics, the reconstruction of regulatory networks has been hindered by the relatively low level of evolutionary conservation of their molecular components. Recent developments in experimental techniques have allowed the generation of vast a...

journal_title：Current opinion in biotechnology

authors： Herrgård MJ,Covert MW,Palsson BØ

更新日期：2004-02-01 00:00:00

abstract：:Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connec...

journal_title：Current opinion in biotechnology

authors： Adrian L,Marco-Urrea E

更新日期：2016-10-01 00:00:00

abstract：:Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...

journal_title：Current opinion in biotechnology

authors： Bjork SM,Joensson HN

更新日期：2019-02-01 00:00:00

abstract：:The commercialization of high-precision microcalorimeters has led to a sharp increase in the acquisition and utilization of calorimetric data on the binding and folding reactions of proteins. This thermodynamic database, together with high-resolution structures of proteins and their complexes with ligands, has facilit...

journal_title：Current opinion in biotechnology

authors： Connelly PR

更新日期：1994-08-01 00:00:00

abstract：:Over the next decade, the impact of library synthesis will play a major role in shortening the lead optimization phase of drug discovery. The prognosis for combinatorial chemistry to discover fundamentally different new classes of therapeutically active small molecules against some of the more difficult biological tar...

journal_title：Current opinion in biotechnology

authors： Myers PL

更新日期：1997-12-01 00:00:00

abstract：:Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...

journal_title：Current opinion in biotechnology

authors： Newton RJ,McClary JS

更新日期：2019-06-01 00:00:00

abstract：:Heterotrophic bacteria are an integral part of any environmental biotechnology process (EBP). Therefore, factors controlling bacterial abundance, activity, and community composition are central to the understanding of such processes. Among these factors, top-down control by bacteriophage predation has so far received ...

journal_title：Current opinion in biotechnology

authors： Shapiro OH,Kushmaro A

更新日期：2011-06-01 00:00:00

abstract：:Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites b...

journal_title：Current opinion in biotechnology

authors： Ohta D,Kanaya S,Suzuki H

更新日期：2010-02-01 00:00:00