Abstract:
:Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites based on their comparison to available authentic compounds. Such metabolomics platforms were mostly based on mass spectrometry (MS) technologies enabled by a combination of different ionization methods together with a variety of separation steps including LC, GC, and CE. Among these, Fourier-transform ion cyclotron resonance MS (FT-ICR/MS) is distinguished from other MS technologies by its ultrahigh resolution power in mass to charge ratio (m/z). The potential of FT-ICR/MS as a distinctive metabolomics tool has been demonstrated in nontargeted metabolic profiling and functional characterization of novel genes. Here, we discuss both the advantages and difficulties encountered in the FT-ICR/MS metabolomics studies.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Ohta D,Kanaya S,Suzuki Hdoi
10.1016/j.copbio.2010.01.012subject
Has Abstractpub_date
2010-02-01 00:00:00pages
35-44issue
1eissn
0958-1669issn
1879-0429pii
S0958-1669(10)00013-3journal_volume
21pub_type
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