Bacteriophage ecology in environmental biotechnology processes.

abstract：:Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding ...

journal_title：Current opinion in biotechnology

authors： Lynch JH,Huang XQ,Dudareva N

更新日期：2021-01-08 00:00:00

abstract：:Starting from experimental data on sequence, structure or biochemical properties of enzymes, protein design seeks to construct enzymes with desired activity, stability, specificity and selectivity. Two strategies are widely used to investigate sequence-structure-function relationships: statistical methods to analyse p...

journal_title：Current opinion in biotechnology

authors： Pleiss J

更新日期：2011-10-01 00:00:00

abstract：:Most secretory proteins are synthesized as precursors with an amino-terminal signal peptide. Genetic identification of proteins essential for signal peptide dependent translocation to the Escherichia coli periplasm has led to the biochemical dissection of the secretion pathway. Additional mechanisms exist in Gram-nega...

journal_title：Current opinion in biotechnology

authors： Gennity JM,Inouye M

更新日期：1991-10-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activiti...

journal_title：Current opinion in biotechnology

authors： Allen DK,Young JD

更新日期：2020-08-01 00:00:00

abstract：:In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

journal_title：Current opinion in biotechnology

authors： Gumpert J,Hoischen C

更新日期：1998-10-01 00:00:00

abstract：:Driven by the quest for sustainability, recent years have seen a tremendous progress in bio-based production routes from renewable raw materials to commercial goods. Particularly, the production of organic acids has crystallized as a competitive and fast-evolving field, related to the broad applicability of organic ac...

journal_title：Current opinion in biotechnology

authors： Becker J,Lange A,Fabarius J,Wittmann C

更新日期：2015-12-01 00:00:00

abstract：:Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify...

journal_title：Current opinion in biotechnology

authors： Kretzschmar T,von Rüden T

更新日期：2002-12-01 00:00:00

abstract：:It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...

journal_title：Current opinion in biotechnology

authors： Kuwajima K

更新日期：1992-10-01 00:00:00

abstract：:Fc fusion proteins are molecules in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. Fc fusion proteins have some antibody-like properties such as long serum half-life and easy expression and purification, making them ...

journal_title：Current opinion in biotechnology

authors： Huang C

更新日期：2009-12-01 00:00:00

abstract：:Adaptive laboratory evolution (ALE) strategies allow for the metabolic engineering of microorganisms by combining genetic variation with the selection of beneficial mutations in an unbiased fashion. These ALE strategies have been proven highly effective in the optimization of production strains. In contrast to rationa...

journal_title：Current opinion in biotechnology

authors： Portnoy VA,Bezdan D,Zengler K

更新日期：2011-08-01 00:00:00

abstract：:With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

journal_title：Current opinion in biotechnology

authors： Kontermann RE

更新日期：2011-12-01 00:00:00

abstract：:Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...

journal_title：Current opinion in biotechnology

authors： Rehemtulla A,Kaufman RJ

更新日期：1992-10-01 00:00:00

abstract：:Intraportal islet transplantation has emerged as a promising treatment for type 1 diabetes mellitus (T1DM). Nevertheless, long-term efficacy has been limited to a marginal number of patients. Outcomes have been restricted, in part, by challenges associated with the transplant site, poor vascularization, and disruption...

journal_title：Current opinion in biotechnology

authors： Coronel MM,Stabler CL

更新日期：2013-10-01 00:00:00

abstract：:Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...

journal_title：Current opinion in biotechnology

authors： Newton RJ,McClary JS

更新日期：2019-06-01 00:00:00

abstract：:Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

journal_title：Current opinion in biotechnology

authors： Tissier A

更新日期：2018-02-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:Considerable progress has been made in understanding the relationship between local amino acid sequence and local protein structure. Recent highlights include numerous studies of the structures adopted by short peptides, new approaches to correlating sequence patterns with structure patterns, and folding simulations u...

journal_title：Current opinion in biotechnology

authors： Bystroff C,Simons KT,Han KF,Baker D

更新日期：1996-08-01 00:00:00

abstract：:Combinatorial biocatalysis is an emerging technology in the field of drug discovery. The biocatalytic approach to combinatorial chemistry uses enzymatic, chemoenzymatic, and microbial transformations to generate libraries from lead compounds. Important recent advances in combinatorial biocatalysis include iterative de...

journal_title：Current opinion in biotechnology

authors： Altreuter DH,Clark DS

更新日期：1999-04-01 00:00:00

abstract：:Saccharomyces cerevisiae has decisive advantages in industrial processes due to its tolerance to alcohols and fermentation conditions. Butanol isomers are considered as suitable fuel substitutes and valuable biomass-derived chemical building blocks. Whereas high production was achieved with bacterial systems, metaboli...

journal_title：Current opinion in biotechnology

authors： Generoso WC,Schadeweg V,Oreb M,Boles E

更新日期：2015-06-01 00:00:00

abstract：:The pathway of monolignol biosynthesis involves many components interacting in a metabolic grid to regulate the supply and ratios of monolignols for lignification. The complexity of the pathway challenges any intuitive prediction of the output without mathematical modeling. Several models have been presented to quanti...

journal_title：Current opinion in biotechnology

authors： Wang JP,Matthews ML,Naik PP,Williams CM,Ducoste JJ,Sederoff RR,Chiang VL

更新日期：2019-04-01 00:00:00

abstract：:Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yie...

journal_title：Current opinion in biotechnology

authors： Wen F,Nair NU,Zhao H

更新日期：2009-08-01 00:00:00

abstract：:Xenopus oocytes have been used as a surrogate genetic system for the study of many facets of gene expression. Some recent salient examples where injected oocytes proved to be indispensable for the analysis of transcription, translation, RNA/protein transport, and ion channel formation are described. ...

journal_title：Current opinion in biotechnology

authors： Richter JD

更新日期：1991-10-01 00:00:00

abstract：:Technoeconomic analysis (TEA) is an approach for conducting process design and simulation, informed by empirical data, to estimate capital costs, operating costs, mass balances, and energy balances for a commercial scale biorefinery. TEA serves as a useful method to screen potential research priorities, identify cost ...

journal_title：Current opinion in biotechnology

authors： Scown CD,Baral NR,Yang M,Vora N,Huntington T

更新日期：2021-01-18 00:00:00

abstract：:Recombinant DNA technology has expanded the potential of the targeted screening approach in drug discovery. The use of cloned and expressed receptors as targets for the evaluation of novel compounds can significantly simplify the structure affinity/activity process. However, the patenting of cloned receptors, as well ...

journal_title：Current opinion in biotechnology

authors： Williams M

更新日期：1993-02-01 00:00:00

abstract：:Biological pulping has the potential to improve the quality of pulp, properties of paper and to reduce energy costs and environmental impact relative to traditional pulping operations. It has been suggested that energy savings alone could make the process economically viable. Other benefits include improved burst stre...

journal_title：Current opinion in biotechnology

authors： Breen A,Singleton FL

更新日期：1999-06-01 00:00:00

abstract：:As the ambitions of synthetic biology approach genome-scale engineering, comprehensive characterization of cellular systems is required, as well as a means to accurately model cell-scale molecular interactions. These requirements are coincident with the goals of systems biology and, thus, systems biology will become t...

journal_title：Current opinion in biotechnology

authors： Barrett CL,Kim TY,Kim HU,Palsson BØ,Lee SY

更新日期：2006-10-01 00:00:00

abstract：:Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted s...

journal_title：Current opinion in biotechnology

authors： Sayler GS,Fleming JT,Nivens DE

更新日期：2001-10-01 00:00:00

abstract：:An intense interest has grown in the unique interactions of nucleic acids with metal ions, which lead to the formation of metal-base pairs and the generation of fluorescent nanomaterials. In this review, different types of metal-base pairs, especially those formed from naturally occurring nucleosides, are described wi...

journal_title：Current opinion in biotechnology

authors： Park KS,Park HG

更新日期：2014-08-01 00:00:00

abstract：:Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...

journal_title：Current opinion in biotechnology

authors： Jewett MC,Hofmann G,Nielsen J

更新日期：2006-04-01 00:00:00