Quality and authenticity of heterologous proteins synthesized in yeast.


:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determining authenticity.


Curr Opin Biotechnol


Eckart MR,Bussineau CM




Has Abstract


1996-10-01 00:00:00














  • Why recombinant antibodies - benefits and applications.

    abstract::Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological conditions. Abs are required for the quantification of targets, dete...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Basu K,Green EM,Cheng Y,Craik CS

    更新日期:2019-12-01 00:00:00

  • Techniques for the experimental investigation of the protein corona.

    abstract::Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Carrillo-Carrion C,Carril M,Parak WJ

    更新日期:2017-08-01 00:00:00

  • The evolution of IncP catabolic plasmids.

    abstract::The recent adoption of whole plasmid genome sequencing as a routine analytical technique has provided the basis for cataloging the historical events through which plasmids are assembled from the available families of modular plasmid components. Horizontal gene transfer mediated by plasmids plays an important role in t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Dennis JJ

    更新日期:2005-06-01 00:00:00

  • Electron-capture dissociation tandem mass spectrometry.

    abstract::Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Zubarev RA

    更新日期:2004-02-01 00:00:00

  • Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture.

    abstract::The increasing demand for a steady, healthy food supply requires an efficient control of the major pests and plant diseases. Current management practices are based largely on the application of synthetic pesticides. The excessive use of agrochemicals has caused serious environmental and health problems. Therefore, the...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pérez-García A,Romero D,de Vicente A

    更新日期:2011-04-01 00:00:00

  • Directed evolution: new parts and optimized function.

    abstract::Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understa...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Dougherty MJ,Arnold FH

    更新日期:2009-08-01 00:00:00

  • Synergistic use of adult and embryonic stem cells to study human hematopoiesis.

    abstract::Embryonic stem cells (ESCs) and adult stem cells both provide important resources to define the mechanisms of hematopoietic cell development. To date, studies that utilize hematopoietic stem cells (HSCs) isolated from sites such as bone marrow or umbilical cord blood have been the primary means to identify molecular a...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Martin CH,Kaufman DS

    更新日期:2005-10-01 00:00:00

  • Protein engineering for molecular electronics.

    abstract::Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Sligar SG,Salemme FR

    更新日期:1992-08-01 00:00:00

  • Directed evolution and synthetic biology applications to microbial systems.

    abstract::Biotechnology applications require engineering complex multi-genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole bio...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bassalo MC,Liu R,Gill RT

    更新日期:2016-06-01 00:00:00

  • The Hox gene family in transgenic mice.

    abstract::Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Krumlauf R

    更新日期:1991-12-01 00:00:00

  • Exploiting molecular motors as nanomachines: the mechanisms of de novo and re-engineered cytoskeletal motors.

    abstract::Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: DelRosso NV,Derr ND

    更新日期:2017-08-01 00:00:00

  • Stabilization of protein structures.

    abstract::The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowled...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lee B,Vasmatzis G

    更新日期:1997-08-01 00:00:00

  • Genetically encoded tools for RNA imaging in living cells.

    abstract::RNA imaging probes help us investigate how transport and dynamics of RNA contribute to subcellular RNA localization or regulation of gene expression. Out of the plethora of strategies that have been developed to image RNA in living cells, genetically encoded probes are interesting because they can be produced by the c...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rath AK,Rentmeister A

    更新日期:2015-02-01 00:00:00

  • Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli.

    abstract::Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hos...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Yuzawa S,Kim W,Katz L,Keasling JD

    更新日期:2012-10-01 00:00:00

  • Engineering and application of polymerases for synthetic genetics.

    abstract::Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nu...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Houlihan G,Arangundy-Franklin S,Holliger P

    更新日期:2017-12-01 00:00:00

  • Engineering solventogenic clostridia.

    abstract::Solventogenic clostridia are strictly anaerobic, endospore forming bacteria that produce a large array of primary metabolites, like butanol, by anaerobically degrading simple and complex carbohydrates, including cellulose and hemicellulose. Two genomes have been sequenced and some genetic tools have been developed, bu...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Papoutsakis ET

    更新日期:2008-10-01 00:00:00

  • Microorganisms relevant to bioremediation.

    abstract::Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Watanabe K

    更新日期:2001-06-01 00:00:00

  • Advances in the genetics of thermophilic lactic acid bacteria.

    abstract::Molecular genetics of thermophilic lactic acid bacteria has advanced in several directions: exploitation of the milk proteins and sugars; primary and secondary metabolism; stress response; and molecular ecology of bacteria and their phages. These have singularly contributed to open new avenues of scientific interest i...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Delcour J,Ferain T,Hols P

    更新日期:2000-10-01 00:00:00

  • DNA nanotechnology with one-dimensional self-assembled nanostructures.

    abstract::The information encoded in the base sequence of DNA provides substantial structural and functional information for the instructive self-assembly of one-dimensional (1D) functional DNA nanostructures. The hybridization chain reaction (HCR) and the formation of HCR-stimulated DNAzyme nanochains are presented, as a means...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wang F,Willner B,Willner I

    更新日期:2013-08-01 00:00:00

  • Continuous integrated manufacturing of therapeutic proteins.

    abstract::With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Karst DJ,Steinebach F,Morbidelli M

    更新日期:2018-10-01 00:00:00

  • Molecular and biotechnological approaches to fish vaccines.

    abstract::Global aquaculture is projected to grow from 10 million to 20 million metric tons by the year 2000. To meet this projection, the aquaculture industry must bring some of its infectious disease problems under control without relying on antibiotics and chemotherapeutics. Thus, vaccines for fish and shellfish are being so...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Leong JA

    更新日期:1993-06-01 00:00:00

  • Targeting split vaccines to the endosome improves vaccination.

    abstract::Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wagner H,Heit A,Schmitz F,Bauer S

    更新日期:2004-12-01 00:00:00

  • Aquatic phototrophs: efficient alternatives to land-based crops for biofuels.

    abstract::To mitigate some of the potentially deleterious environmental and agricultural consequences associated with current land-based-biofuel feedstocks, we propose the use of biofuels derived from aquatic microbial oxygenic photoautotrophs (AMOPs), more commonly known as cyanobacteria, algae, and diatoms. Herein we review t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Dismukes GC,Carrieri D,Bennette N,Ananyev GM,Posewitz MC

    更新日期:2008-06-01 00:00:00

  • Steps towards 'drop-in' biofuels: focusing on metabolic pathways.

    abstract::The past decade has witnessed rapid advance in microbial production of 'drop-in' biofuels from renewable resources. Various biosynthetic pathways have been constructed to produce biofuels with diverse structures, and multiple metabolic engineering strategies have been developed to increase biofuel titers, yields, prod...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Jiang W,Gu P,Zhang F

    更新日期:2018-10-01 00:00:00

  • Bioavailability of pollutants and chemotaxis.

    abstract::The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Krell T,Lacal J,Reyes-Darias JA,Jimenez-Sanchez C,Sungthong R,Ortega-Calvo JJ

    更新日期:2013-06-01 00:00:00

  • Extracellular production of recombinant proteins using bacterial autotransporters.

    abstract::Escherichia coli is still a very popular host for the production of recombinant proteins at an analytical or industrial scale. Secretion of the proteins into the culture medium or display at the cell surface would be preferred in many applications but is hampered by the complex two-layered cell envelope. The autotrans...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Jong WS,Saurí A,Luirink J

    更新日期:2010-10-01 00:00:00

  • Ligand-binding domain of estrogen receptors.

    abstract::Estrogen receptors are multi-domain proteins that interact with other proteins and DNA to fulfil their function: the regulation of transcription. During the past 2-3 years, our understanding of this complex process has increased tremendously as crystal structures of isolated ligand-binding domains in complex with vari...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Mueller-Fahrnow A,Egner U

    更新日期:1999-12-01 00:00:00

  • Optimizing the fluorescent protein toolbox and its use.

    abstract::Fluorescent proteins (FPs) and fluorescent protein-derived biosensors are indispensable tools in life sciences. They make it possible to probe the location, activity, or interaction of molecules of interest from a subcellular to a multicellular scale. The desire for high-resolution and multidimensional information imp...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Duwé S,Dedecker P

    更新日期:2019-08-01 00:00:00

  • Technical issues in the large-scale hydrothermal liquefaction of microalgal biomass to biocrude.

    abstract::Much of the current knowledge on the hydrothermic liquefaction of biomass to biocrude is on the basis of laboratory benchtop findings, and the step up to industrial scale reactors will require a range of information that is currently either unavailable or insufficient. This work highlights a number of these issues suc...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lee A,Lewis D,Kalaitzidis T,Ashman P

    更新日期:2016-04-01 00:00:00

  • The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology.

    abstract::Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kildegaard HF,Baycin-Hizal D,Lewis NE,Betenbaugh MJ

    更新日期:2013-12-01 00:00:00