Sample treatment procedures for environmental sensing and biosensing.

abstract：:Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

journal_title：Current opinion in biotechnology

authors： Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

更新日期：2019-04-01 00:00:00

abstract：:In the past year, significant progress in the field of gene transfer has been made possible by refinement of the technique of particle bombardment. The process has been utilized for the study of gene expression in plastids and mitochondria, the production of transgenic crop plants and gene transfer into live animals. ...

journal_title：Current opinion in biotechnology

authors： Klein TM,Fitzpatrick-McElligott S

更新日期：1993-10-01 00:00:00

abstract：:Life depends on the interaction of proteins. The availability of the complete human genome sequence has highlighted the need for a tool to analyse protein interactions and several databases have been compiled for this purpose. These databases document, categorize, and analyze interacting proteins and the cellular func...

journal_title：Current opinion in biotechnology

authors： Xenarios I,Eisenberg D

更新日期：2001-08-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...

journal_title：Current opinion in biotechnology

authors： Labrou NE,Papageorgiou AC,Pavli O,Flemetakis E

更新日期：2015-04-01 00:00:00

abstract：:Microbial communities are essential for most ecosystem processes and interact in highly complex ways with virtually all eukaryotes. Thus, a detailed understanding of the function of such communities is a fundamental prerequisite for microbial ecologists, applied microbiologists and microbiome researchers. Using single...

journal_title：Current opinion in biotechnology

authors： Wang Y,Huang WE,Cui L,Wagner M

更新日期：2016-10-01 00:00:00

abstract：:Our ability to dissect and understand the principles of gene regulatory circuits is partly limited by the resolution of our experimental assays. In this brief review, we discuss aspects of gene expression in microbial organisms apparent only when increasing the experimental resolution from populations to single cells ...

journal_title：Current opinion in biotechnology

authors： Wallden M,Elf J

更新日期：2011-02-01 00:00:00

abstract：:The use of novel materials in the nano-scale size range for applications in devices, drugs and diagnostic agents comes with a number of new opportunities, and also serious challenges to human applications. The larger size of particulate-based agents, as compared to traditional drugs, allows for the significant advanta...

journal_title：Current opinion in biotechnology

authors： Scheinberg DA,Grimm J,Heller DA,Stater EP,Bradbury M,McDevitt MR

更新日期：2017-08-01 00:00:00

abstract：:Antibacterial strategies targeting bacterial adhesion to substrates are considered a valuable alternative to traditional antibiotic therapy, in view of the great advantage they bring in combating the infectious process at the very early stage without selecting for drug resistant cells. Amongst bioactive compounds with...

journal_title：Current opinion in biotechnology

authors： Signoretto C,Canepari P,Stauder M,Vezzulli L,Pruzzo C

更新日期：2012-04-01 00:00:00

abstract：:Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destru...

journal_title：Current opinion in biotechnology

authors： Wallace CJ

更新日期：1995-08-01 00:00:00

abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

journal_title：Current opinion in biotechnology

authors： Pais DA,Carrondo MJ,Alves PM,Teixeira AP

更新日期：2014-12-01 00:00:00

abstract：:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for preci...

journal_title：Current opinion in biotechnology

authors： Tolle F,Stücheli P,Fussenegger M

更新日期：2019-10-01 00:00:00

abstract：:Heterogeneity of industrial production cultures is accepted to a certain degree; however, the underlying mechanisms are seldom perceived or included in the development of new bioprocess control strategies. Population heterogeneity and its basics, perceptible in the diverse proficiency of cells, begins with asymmetric ...

journal_title：Current opinion in biotechnology

authors： Müller S,Harms H,Bley T

更新日期：2010-02-01 00:00:00

abstract：:Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

journal_title：Current opinion in biotechnology

authors： Meyer RD,Cook D

更新日期：2000-02-01 00:00:00

abstract：:Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...

journal_title：Current opinion in biotechnology

authors： Zubarev RA

更新日期：2004-02-01 00:00:00

abstract：:Is structure, rather than sequence, the key to the successful generation of truly novel proteins? While protein evolution by homologous recombination has become an established tool to explore confined regions in sequence space, the generation of functional hybrid proteins by homology-independent methods further expand...

journal_title：Current opinion in biotechnology

authors： Lutz S,Benkovic SJ

更新日期：2000-08-01 00:00:00

abstract：:Fermentation of syngas is a means through which unutilized organic waste streams can be converted biologically into biofuels and commodity chemicals. Despite recent advances, several issues remain which limit implementation of industrial-scale syngas fermentation processes. At the cellular level, the energy conservati...

journal_title：Current opinion in biotechnology

authors： Latif H,Zeidan AA,Nielsen AT,Zengler K

更新日期：2014-06-01 00:00:00

abstract：:The worldwide emergence of nanoscale science and engineering was marked by the announcement of the National Nanotechnology Initiative (NNI) in January 2000. Recent research on biosystems at the nanoscale has created one of the most dynamic science and technology domains at the confluence of physical sciences, molecula...

journal_title：Current opinion in biotechnology

authors： Roco MC

更新日期：2003-06-01 00:00:00

abstract：:Based on projections for global population growth, current techniques for improving agricultural yields will not be able to address future demands for major food crops. Improving photosynthetic efficiency by engineering carbon fixation has been identified as one of the most important approaches for increasing agricult...

journal_title：Current opinion in biotechnology

authors： Giessen TW,Silver PA

更新日期：2017-08-01 00:00:00

abstract：:Genetic engineering for complex or combined traits requires the simultaneous expression of multiple genes, and has been considered as the bottleneck for the next generation of genetic engineering in plants. Minichromosome technology provides one solution to the stable expression and maintenance of multiple transgenes ...

journal_title：Current opinion in biotechnology

authors： Yu W,Han F,Birchler JA

更新日期：2007-10-01 00:00:00

abstract：:Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...

journal_title：Current opinion in biotechnology

authors： Hazen TC,Rocha AM,Techtmann SM

更新日期：2013-06-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:Over the next decade, the impact of library synthesis will play a major role in shortening the lead optimization phase of drug discovery. The prognosis for combinatorial chemistry to discover fundamentally different new classes of therapeutically active small molecules against some of the more difficult biological tar...

journal_title：Current opinion in biotechnology

authors： Myers PL

更新日期：1997-12-01 00:00:00

abstract：:Plants synthesize a myriad of secondary metabolites (SMs) that are derived from central or primary metabolism. While these so-called natural products have been targets for plant metabolic engineering attempts for many years, the immense value of manipulating the interface between committed steps in secondary metabolis...

journal_title：Current opinion in biotechnology

authors： Aharoni A,Galili G

更新日期：2011-04-01 00:00:00

abstract：:The nature of DNA, the sequence of the human genome and our increased understanding of the genetic basis of many inherited and acquired disorders have made the possibility of curing diseases a reality. The modulation of a host's genome is now the ultimate goal in the treatment of genetic diseases. Historically, gene t...

journal_title：Current opinion in biotechnology

authors： Kren BT,Steer CJ

更新日期：2002-10-01 00:00:00

abstract：:Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...

journal_title：Current opinion in biotechnology

authors： Sligar SG,Salemme FR

更新日期：1992-08-01 00:00:00

abstract：:Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgen...

journal_title：Current opinion in biotechnology

authors： Somers DA,Makarevitch I

更新日期：2004-04-01 00:00:00

abstract：:Biological pulping has the potential to improve the quality of pulp, properties of paper and to reduce energy costs and environmental impact relative to traditional pulping operations. It has been suggested that energy savings alone could make the process economically viable. Other benefits include improved burst stre...

journal_title：Current opinion in biotechnology

authors： Breen A,Singleton FL

更新日期：1999-06-01 00:00:00

abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...

journal_title：Current opinion in biotechnology

authors： Schwager J,Mohajeri MH,Fowler A,Weber P

更新日期：2008-04-01 00:00:00

abstract：:Obtaining an abundant supply of human cells with specific differentiated properties is a key issue in many research studies and biotechnology applications. Recent advances in this area include the finding that forced expression of the enzyme telomerase can greatly increase the proliferative potential of at least some ...

journal_title：Current opinion in biotechnology

authors： Yeager TR,Reddel RR

更新日期：1999-10-01 00:00:00