Transgene integration in plants: poking or patching holes in promiscuous genomes?

abstract：:Bioremediation has historically been approached as a 'black box' in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at t...

journal_title：Current opinion in biotechnology

authors： Chakraborty R,Wu CH,Hazen TC

更新日期：2012-06-01 00:00:00

abstract：:Many significant advances have occurred recently in the field of protein electrophoresis and related technologies. Improvements have been made in capillary electrophoresis in apparatus design, detection, and capillary modification with coatings and fillers. The transfer of proteins to a blot, the capacity to analyze a...

journal_title：Current opinion in biotechnology

authors： Zewert TE,Harrington MG

更新日期：1993-02-01 00:00:00

abstract：:Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies...

journal_title：Current opinion in biotechnology

authors： Suter B,Kittanakom S,Stagljar I

更新日期：2008-08-01 00:00:00

abstract：:Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

journal_title：Current opinion in biotechnology

authors： Wagner H,Heit A,Schmitz F,Bauer S

更新日期：2004-12-01 00:00:00

abstract：:NMR methods in drug discovery have traditionally been used to obtain structural information for drug targets or target-ligand complexes. Recently, it has been shown that NMR may be used as an alternative approach for identification of ligands that bind to protein drug targets, shifting the emphasis of many NMR laborat...

journal_title：Current opinion in biotechnology

authors： Moore JM

更新日期：1999-02-01 00:00:00

abstract：:Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...

journal_title：Current opinion in biotechnology

authors： Jewett MC,Hofmann G,Nielsen J

更新日期：2006-04-01 00:00:00

abstract：:Thiopeptide antibiotics, which are a class of sulfur-rich and highly modified peptide natural products, exhibit a wide variety of important biological properties. These antibiotics are ribosomally synthesized and arise from post-translational modifications, exemplifying a process through which nature develops the stru...

journal_title：Current opinion in biotechnology

authors： Lin Z,He Q,Liu W

更新日期：2017-12-01 00:00:00

abstract：:Recent field and laboratory studies have evaluated the potential for aerobic co-metabolism of chlorinated solvents. Different co-metabolic substrates and different methods of application have been tried, including growing indigenous microbes in situ, and injecting into the soil subsurface strains grown in subsurface r...

journal_title：Current opinion in biotechnology

authors： Semprini L

更新日期：1997-06-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:In recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. It has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ESTs had limited impact for breeding. Until recently, the models used by ...

journal_title：Current opinion in biotechnology

authors： Dal-Bianco M,Carneiro MS,Hotta CT,Chapola RG,Hoffmann HP,Garcia AA,Souza GM

更新日期：2012-04-01 00:00:00

abstract：:To mitigate some of the potentially deleterious environmental and agricultural consequences associated with current land-based-biofuel feedstocks, we propose the use of biofuels derived from aquatic microbial oxygenic photoautotrophs (AMOPs), more commonly known as cyanobacteria, algae, and diatoms. Herein we review t...

journal_title：Current opinion in biotechnology

authors： Dismukes GC,Carrieri D,Bennette N,Ananyev GM,Posewitz MC

更新日期：2008-06-01 00:00:00

abstract：:Fungal ligninolytic enzymes have broad biotechnological applications. Particularly laccases and certain fungal class II peroxidases from white-rot basidiomycetes are considered in degradation of persistent organic pollutants. Promising processes with reusable immobilized laccases in special reactors have been develope...

journal_title：Current opinion in biotechnology

authors： Kües U

更新日期：2015-06-01 00:00:00

abstract：:Population-level studies enabled by high-throughput phenotyping have revealed significant variation in lignin characteristics including content, S:G:H ratio, inter-unit linkage distributions, and molecular weights across multiple plant species. Coupled with genome-wide association mapping studies (GWAS) targeted at li...

journal_title：Current opinion in biotechnology

authors： Tuskan GA,Muchero W,Tschaplinski TJ,Ragauskas AJ

更新日期：2019-04-01 00:00:00

abstract：:Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1-200 microm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit inc...

journal_title：Current opinion in biotechnology

authors： Govardhan CP

更新日期：1999-08-01 00:00:00

abstract：:Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for ...

journal_title：Current opinion in biotechnology

authors： Besada-Lombana PB,McTaggart TL,Da Silva NA

更新日期：2018-10-01 00:00:00

abstract：:Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

journal_title：Current opinion in biotechnology

authors： Kim NM,Sinnott RW,Sandoval NR

更新日期：2020-08-01 00:00:00

abstract：:Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...

journal_title：Current opinion in biotechnology

authors： Vanholme B,El Houari I,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:The structure determinations of the cytochrome bc1 complex and the prokaryotic potassium channel demonstrate that a wider range of membrane proteins are now amenable to study by X-ray crystallography. Furthermore, the structures of porins and interfacial membrane proteins show that membrane structural biology is becom...

journal_title：Current opinion in biotechnology

authors： Garavito RM

更新日期：1998-08-01 00:00:00

abstract：:Silk combines biological properties, such as non-toxicity and biodegradability, with physico-chemical ones, for example, mechanical strength. Base on molecular engineering, nowadays also new non-silk functions can be implemented in silk materials. Driven by rational design and ingenuity, innovative recombinant silk pr...

journal_title：Current opinion in biotechnology

authors： Saric M,Scheibel T

更新日期：2019-12-01 00:00:00

abstract：:Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent...

journal_title：Current opinion in biotechnology

authors： Stoloff DH,Wanunu M

更新日期：2013-08-01 00:00:00

abstract：:Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...

journal_title：Current opinion in biotechnology

authors： Zubarev RA

更新日期：2004-02-01 00:00:00

abstract：:Oil prices and government mandates have catalyzed rapid growth of nonfossil transportation fuels in recent years, with a large focus on ethanol from energy crops, but the food crops used as first-generation energy crops today are not optimized for this purpose. We show that the theoretical efficiency of conversion of ...

journal_title：Current opinion in biotechnology

authors： Heaton EA,Flavell RB,Mascia PN,Thomas SR,Dohleman FG,Long SP

更新日期：2008-06-01 00:00:00

abstract：:Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactio...

journal_title：Current opinion in biotechnology

authors： Jaeger KE,Eggert T

更新日期：2004-08-01 00:00:00

abstract：:The concept of whole genome amplification is something that has arisen in the past few years as the polymerase chain reaction (PCR) has been adapted to replicate regions of genomes that are of biological interest. The applications are many--forensic science, embryonic disease diagnosis, bioterrorism genome detection, ...

journal_title：Current opinion in biotechnology

authors： Hawkins TL,Detter JC,Richardson PM

更新日期：2002-02-01 00:00:00

abstract：:Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....

journal_title：Current opinion in biotechnology

authors： Krumlauf R

更新日期：1991-12-01 00:00:00

abstract：:The recent technological advances in next generation sequencing have brought the field closer to the goal of reconstructing all genomes within a community by presenting high throughput sequencing at much lower costs. While these next-generation sequencing technologies have allowed a massive increase in available raw s...

journal_title：Current opinion in biotechnology

authors： Scholz MB,Lo CC,Chain PS

更新日期：2012-02-01 00:00:00

abstract：:Vaccines against mutating pathogens such as influenza, HIV, or plasmodium are poorly protective towards new evolving strains. Rare individuals naturally mount broadly neutralizing antibodies covering most strains, but the requirements for their induction are unknown. The antibody response to vaccination has been recap...

journal_title：Current opinion in biotechnology

authors： Robert PA,Marschall AL,Meyer-Hermann M

更新日期：2018-06-01 00:00:00

abstract：:The development of new strategies for the delivery of vaccine antigens or immune modulators to the mucosal tissue includes innovative approaches such as the use of genetically modified food microorganisms and plants. Even though the 'proof-of-concept' has recently been established for these two systems, key questions ...

journal_title：Current opinion in biotechnology

authors： Mercenier A,Wiedermann U,Breiteneder H

更新日期：2001-10-01 00:00:00

abstract：:Poxviruses are widely used for the cytoplasmic expression of recombinant genes in mammalian cells. Recent improvements allow high expression and simplify the integration of multiple foreign genes. Vaccinia virus mutants and other poxviruses that undergo abortive infection in mammalian cells are receiving special atten...

journal_title：Current opinion in biotechnology

authors： Carroll MW,Moss B

更新日期：1997-10-01 00:00:00

abstract：:Adenoviral vectors have proven to be excellent vehicles for gene delivery in vivo to a wide range of cell types. These vectors have been used to transfer genes such as CFTR to correct the defect in cystic fibrosis and, more recently, to supply serum blood factors and genetically modify tumors to enhance therapy. ...

journal_title：Current opinion in biotechnology

authors： Bramson JL,Graham FL,Gauldie J

更新日期：1995-10-01 00:00:00