Abstract:
:Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high-throughput screens on non-growth-related phenotypes. While these inducible systems, primarily transcription factor/promoter pairs, have been utilized extensively in Escherichia coli, progress in other bacteria is limited because of differences in transcription machinery, physiological compatibility of parts and proteins, and other nuances. Here, we provide an overview of the available genetic biosensing elements in non-model organisms and state-of-the-art efforts to engineer them, and then discuss challenges preventing these methods from common use in non-model bacteria.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Kim NM,Sinnott RW,Sandoval NRdoi
10.1016/j.copbio.2019.09.009subject
Has Abstractpub_date
2020-08-01 00:00:00pages
39-46eissn
0958-1669issn
1879-0429pii
S0958-1669(19)30078-3journal_volume
64pub_type
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