Novel biotechnological approaches to produce biological compounds: challenges and opportunities for science communication.

abstract：:Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites b...

journal_title：Current opinion in biotechnology

authors： Ohta D,Kanaya S,Suzuki H

更新日期：2010-02-01 00:00:00

abstract：:The selection of an expression platform for recombinant biopharmaceuticals is often centered upon suitable product titers and critical quality attributes, including post-translational modifications. Although notable differences between microbial, yeast, plant, and mammalian host systems exist, recent advances have gre...

journal_title：Current opinion in biotechnology

authors： Meehl MA,Stadheim TA

更新日期：2014-12-01 00:00:00

abstract：:Much of the current knowledge on the hydrothermic liquefaction of biomass to biocrude is on the basis of laboratory benchtop findings, and the step up to industrial scale reactors will require a range of information that is currently either unavailable or insufficient. This work highlights a number of these issues suc...

journal_title：Current opinion in biotechnology

authors： Lee A,Lewis D,Kalaitzidis T,Ashman P

更新日期：2016-04-01 00:00:00

abstract：:The efficient and safe delivery of therapeutic proteins is the key to commercial success and, in some cases, the demonstration of efficacy in current and future biotechnology products. Numerous delivery technologies and companies have evolved over the past year. To critically evaluate the available options, each metho...

journal_title：Current opinion in biotechnology

authors： Cleland JL,Daugherty A,Mrsny R

更新日期：2001-04-01 00:00:00

abstract：:Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological conditions. Abs are required for the quantification of targets, dete...

journal_title：Current opinion in biotechnology

authors： Basu K,Green EM,Cheng Y,Craik CS

更新日期：2019-12-01 00:00:00

abstract：:Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

journal_title：Current opinion in biotechnology

authors： Friedrich MW

更新日期：2006-02-01 00:00:00

abstract：:Reductive catalytic fractionation (RCF) of lignocellulose is an emerging biorefinery scheme that combines biomass fractionation with lignin depolymerisation. Central to this scheme is the integration of heterogeneous catalysis, which overcomes the tendency of lignin to repolymerise. Ultimately, this leads to a low-Mw ...

journal_title：Current opinion in biotechnology

authors： Renders T,Van den Bossche G,Vangeel T,Van Aelst K,Sels B

更新日期：2019-04-01 00:00:00

abstract：:Plant biotechnology has been around since the advent of humankind, resulting in tremendous improvements in plant cultivation through crop domestication, breeding and selection. The emergence of transgenic approaches involving the introduction of defined DNA sequences into plants by humans has rapidly changed the surfa...

journal_title：Current opinion in biotechnology

authors： Francis D,Finer JJ,Grotewold E

更新日期：2017-04-01 00:00:00

abstract：:Starting from experimental data on sequence, structure or biochemical properties of enzymes, protein design seeks to construct enzymes with desired activity, stability, specificity and selectivity. Two strategies are widely used to investigate sequence-structure-function relationships: statistical methods to analyse p...

journal_title：Current opinion in biotechnology

authors： Pleiss J

更新日期：2011-10-01 00:00:00

abstract：:Recombinant proteins that have survived the challenges of process development and clinical trials are becoming blockbuster medical products. Growth factors, enzymes and antibodies are being improved by mutational approaches, fused with other proteins, and even chemically modified in vitro. Drug development and testing...

journal_title：Current opinion in biotechnology

authors： Koths K

更新日期：1995-12-01 00:00:00

abstract：:Fifteen years have passed since T-cell receptor (TCR) genes were identified (reviewed in [1]). Unlike the situation for antibodies, no direct structural information on the TCR proteins has been available for most of this time. Recently, however, the crystal structures of isolated alpha and beta chains were determined,...

journal_title：Current opinion in biotechnology

authors： Garcia KC,Teyton L

更新日期：1998-08-01 00:00:00

abstract：:Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UB...

journal_title：Current opinion in biotechnology

authors： Lee KH,Hamashima K,Kimoto M,Hirao I

更新日期：2018-06-01 00:00:00

abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...

journal_title：Current opinion in biotechnology

authors： McGrath SP,Zhao FJ

更新日期：2003-06-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...

journal_title：Current opinion in biotechnology

authors： Kuwajima K

更新日期：1992-10-01 00:00:00

abstract：:Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels. In the past decade, novel hydrocar...

journal_title：Current opinion in biotechnology

authors： Liu K,Li S

更新日期：2020-04-01 00:00:00

abstract：:Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactio...

journal_title：Current opinion in biotechnology

authors： Jaeger KE,Eggert T

更新日期：2004-08-01 00:00:00

abstract：:Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...

journal_title：Current opinion in biotechnology

authors： Liu Y,Nielsen J

更新日期：2019-12-01 00:00:00

abstract：:Water deficit or drought stress is a major limitation to crop production globally. Plant breeders have used a wide range of technologies to successfully breed varieties that perform well under the growth conditions for their target environments but they are always seeking new opportunities to enhance rates of genetic ...

journal_title：Current opinion in biotechnology

authors： Langridge P,Reynolds MP

更新日期：2015-04-01 00:00:00

abstract：:The gastrointestinal (GI) tract is a complex system characterized by multiple cell types with a determined architectural arrangement. Tissue engineering of the GI tract aims to reinstate the architecture and function of all structural layers. The key point for successful tissue regeneration includes the use of cells/b...

journal_title：Current opinion in biotechnology

authors： Bitar KN,Zakhem E

更新日期：2013-10-01 00:00:00

abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...

journal_title：Current opinion in biotechnology

authors： O'Malley J,Woltjen K,Kaji K

更新日期：2009-10-01 00:00:00

abstract：:Poxviruses are widely used for the cytoplasmic expression of recombinant genes in mammalian cells. Recent improvements allow high expression and simplify the integration of multiple foreign genes. Vaccinia virus mutants and other poxviruses that undergo abortive infection in mammalian cells are receiving special atten...

journal_title：Current opinion in biotechnology

authors： Carroll MW,Moss B

更新日期：1997-10-01 00:00:00

abstract：:Metabolic engineering is not another form of classic manipulation of intermediary metabolism; rather, it is the purposeful design of metabolic networks. The metabolic engineering approach examines biochemical reactions in their entirety, rather than individually, and is concerned with the construction of novel pathway...

journal_title：Current opinion in biotechnology

authors： Stephanopoulos G

更新日期：1994-02-01 00:00:00

abstract：:RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

journal_title：Current opinion in biotechnology

authors： Rossi JJ

更新日期：1992-02-01 00:00:00

abstract：:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

journal_title：Current opinion in biotechnology

authors： Eckart MR,Bussineau CM

更新日期：1996-10-01 00:00:00

abstract：:Driven by the demand to enhance the economic balance of chemical processes, the transformation of a racemate into a single stereoisomer (the deracemization process) is one of the most important areas of research. The recent development of the combined use of enzyme and metal catalysis has led to efficient deracemizati...

journal_title：Current opinion in biotechnology

authors： Pàmies O,Bäckvall JE

更新日期：2003-08-01 00:00:00

abstract：:Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

journal_title：Current opinion in biotechnology

authors： Tissier A

更新日期：2018-02-01 00:00:00

abstract：:Technoeconomic analysis (TEA) is an approach for conducting process design and simulation, informed by empirical data, to estimate capital costs, operating costs, mass balances, and energy balances for a commercial scale biorefinery. TEA serves as a useful method to screen potential research priorities, identify cost ...

journal_title：Current opinion in biotechnology

authors： Scown CD,Baral NR,Yang M,Vora N,Huntington T

更新日期：2021-01-18 00:00:00

abstract：:A mechanistic understanding of gene regulatory network dynamics requires quantitative single-cell data of multiple network components in response to well-defined perturbations. Recent advances in the development of fluorescent biomarkers for proteins, detection of RNA and interactions, microfluidic technology, and hig...

journal_title：Current opinion in biotechnology

authors： Selimkhanov J,Hasty J,Tsimring LS

更新日期：2012-02-01 00:00:00

abstract：:Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an...

journal_title：Current opinion in biotechnology

authors： Bock R

更新日期：2007-04-01 00:00:00