Abstract:
:Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UBP formation, new components with different physicochemical properties from those of the natural ones can be introduced into nucleic acids and proteins site-specifically, providing their increased functionalities. Here, we describe the genetic alphabet expansion technology by focusing on three types of UBPs, which were recently applied to the creations of DNA aptamers that bind to proteins and cells and semi-synthetic organisms containing DNAs with a six-letter alphabet.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Lee KH,Hamashima K,Kimoto M,Hirao Idoi
10.1016/j.copbio.2017.09.006subject
Has Abstractpub_date
2018-06-01 00:00:00pages
8-15eissn
0958-1669issn
1879-0429pii
S0958-1669(17)30136-2journal_volume
51pub_type
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