Genetic circuitry for personalized human cell therapy.


:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for precise spatiotemporal control of target gene expression in living cells. Designer cells can be equipped with advanced gene circuitry enabling them to react precisely to pre-programmed combinations of conditions, automatically triggering a specified response, such as therapeutic protein production. Such cells are promising therapeutic modalities for applications where traditional medical treatments have limitations. Herein, we highlight selected recent examples of designer cells with engineered gene circuits targeted toward applications in personalized human medicine.


Curr Opin Biotechnol


Tolle F,Stücheli P,Fussenegger M




Has Abstract


2019-10-01 00:00:00












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    authors: Kiel C,Serrano L

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  • Microfluidics for cell factory and bioprocess development.

    abstract::Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...

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  • Recent advances in single-cell studies of gene regulation.

    abstract::A mechanistic understanding of gene regulatory network dynamics requires quantitative single-cell data of multiple network components in response to well-defined perturbations. Recent advances in the development of fluorescent biomarkers for proteins, detection of RNA and interactions, microfluidic technology, and hig...

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    authors: Selimkhanov J,Hasty J,Tsimring LS

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  • Technical issues in the large-scale hydrothermal liquefaction of microalgal biomass to biocrude.

    abstract::Much of the current knowledge on the hydrothermic liquefaction of biomass to biocrude is on the basis of laboratory benchtop findings, and the step up to industrial scale reactors will require a range of information that is currently either unavailable or insufficient. This work highlights a number of these issues suc...

    journal_title:Current opinion in biotechnology

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    authors: Lee A,Lewis D,Kalaitzidis T,Ashman P

    更新日期:2016-04-01 00:00:00

  • Nucleic acids in the environment.

    abstract::The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

    journal_title:Current opinion in biotechnology

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    authors: Trevors JT

    更新日期:1996-06-01 00:00:00

  • Receptor-Fc fusion therapeutics, traps, and MIMETIBODY technology.

    abstract::Fc fusion proteins are molecules in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. Fc fusion proteins have some antibody-like properties such as long serum half-life and easy expression and purification, making them ...

    journal_title:Current opinion in biotechnology

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    authors: Huang C

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  • Nature's recyclers: anaerobic microbial communities drive crude biomass deconstruction.

    abstract::Microbial communities within anaerobic ecosystems have evolved to degrade and recycle carbon throughout the earth. A number of strains have been isolated from anaerobic microbial communities, which are rich in carbohydrate active enzymes (CAZymes) to liberate fermentable sugars from crude plant biomass (lignocellulose...

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    authors: Lillington SP,Leggieri PA,Heom KA,O'Malley MA

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    abstract::Driven by the demand to enhance the economic balance of chemical processes, the transformation of a racemate into a single stereoisomer (the deracemization process) is one of the most important areas of research. The recent development of the combined use of enzyme and metal catalysis has led to efficient deracemizati...

    journal_title:Current opinion in biotechnology

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    authors: Pàmies O,Bäckvall JE

    更新日期:2003-08-01 00:00:00

  • Nanophotonic label-free biosensors for environmental monitoring.

    abstract::The field of environmental monitoring has experienced a substantial progress in the last years but still the on-site control of contaminants is an elusive problem. In addition, the growing number of pollutant sources is accompanied by an increasing need of having efficient early warning systems. Several years ago bios...

    journal_title:Current opinion in biotechnology

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    authors: Chocarro-Ruiz B,Fernández-Gavela A,Herranz S,Lechuga LM

    更新日期:2017-06-01 00:00:00

  • The role of anaerobic digestion in the emerging energy economy.

    abstract::Anaerobic digestion is the default process for biological conversion of residue organics to renewable energy and biofuel in the form of methane. However, its scope of application is expanding, due to availability of new technologies, and the emerging drivers of energy and nutrient conservation and recovery. Here, we o...

    journal_title:Current opinion in biotechnology

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    authors: Batstone DJ,Virdis B

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  • Stable-isotope probing of DNA: insights into the function of uncultivated microorganisms from isotopically labeled metagenomes.

    abstract::Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

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    authors: Friedrich MW

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    abstract::Fungal bioactive polysaccharides deriving mainly from the Basidiomycetes family (and some from the Ascomycetes) and medicinal mushrooms have been well known and widely used in far Asia as part of traditional diet and medicine, and in the last decades have been the core of intense research for the understanding and the...

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    authors: Giavasis I

    更新日期:2014-04-01 00:00:00

  • Microfluidic platforms for the dynamic characterisation of synthetic circuitry.

    abstract::Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically...

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    authors: Prangemeier T,Lehr FX,Schoeman RM,Koeppl H

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  • Environmental monitoring using autonomous vehicles: a survey of recent searching techniques.

    abstract::Autonomous vehicles are becoming an essential tool in a wide range of environmental applications that include ambient data acquisition, remote sensing, and mapping of the spatial extent of pollutant spills. Among these applications, pollution source localization has drawn increasing interest due to its scientific and ...

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    authors: Bayat B,Crasta N,Crespi A,Pascoal AM,Ijspeert A

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  • Flux analysis in plant metabolic networks: increasing throughput and coverage.

    abstract::Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

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    authors: Junker BH

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  • Quality and authenticity of heterologous proteins synthesized in yeast.

    abstract::Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

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    authors: Eckart MR,Bussineau CM

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  • Use of cell wall-less bacteria (L-forms) for efficient expression and secretion of heterologous gene products.

    abstract::In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

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    authors: Gumpert J,Hoischen C

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    abstract::Thiopeptide antibiotics, which are a class of sulfur-rich and highly modified peptide natural products, exhibit a wide variety of important biological properties. These antibiotics are ribosomally synthesized and arise from post-translational modifications, exemplifying a process through which nature develops the stru...

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    abstract::Many of the gene products of completely sequenced organisms are 'hypothetical' - they cannot be related to any previously characterized proteins - and so are of completely unknown function. Structural studies provide one means of obtaining functional information in these cases. A 'structural genomics' project has been...

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    authors: Eisenstein E,Gilliland GL,Herzberg O,Moult J,Orban J,Poljak RJ,Banerjei L,Richardson D,Howard AJ

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  • Towards real-time monitoring of therapeutic protein quality in mammalian cell processes.

    abstract::Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

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    authors: Watanabe K

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    authors: Breen A,Singleton FL

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    abstract::With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

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  • Recent trends in metabolic engineering of microbial chemical factories.

    abstract::Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...

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    authors: Liu Y,Nielsen J

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  • Engineering microbial cell morphology and membrane homeostasis toward industrial applications.

    abstract::Microbial cell factory performance is significantly affected by the cell morphology and membrane homeostasis. It is important to ensure that cell factories are able to regulate cell morphology and maintain membrane homeostasis. Cell morphology can be controlled by regulating the formation of elongasomes and divisomes,...

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    abstract::Steady increases in the average sulfur content of petroleum and stricter environmental regulations concerning the sulfur content have promoted studies of bioprocessing to upgrade fossil fuels. Bioprocesses can potentially provide a solution to the need for improved and expanded fuel upgrading worldwide, because biopro...

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    authors: Garavito RM

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