Genetic engineering for fungal and bacterial diseases.

abstract：:Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...

journal_title：Current opinion in biotechnology

authors： Kildegaard HF,Baycin-Hizal D,Lewis NE,Betenbaugh MJ

更新日期：2013-12-01 00:00:00

abstract：:Much progress has been made in the development of techniques for constructing dense grids either of ligands, such as peptides and oligonucleotides, or of cloned nucleic acids. Such arrays are finding practical applications in the analysis of sequence variation and gene expression. Methods for carrying out large number...

journal_title：Current opinion in biotechnology

authors： Southern EM

更新日期：1996-02-01 00:00:00

abstract：:Driven by the quest for sustainability, recent years have seen a tremendous progress in bio-based production routes from renewable raw materials to commercial goods. Particularly, the production of organic acids has crystallized as a competitive and fast-evolving field, related to the broad applicability of organic ac...

journal_title：Current opinion in biotechnology

authors： Becker J,Lange A,Fabarius J,Wittmann C

更新日期：2015-12-01 00:00:00

abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

journal_title：Current opinion in biotechnology

authors： Pais DA,Carrondo MJ,Alves PM,Teixeira AP

更新日期：2014-12-01 00:00:00

abstract：:Academic microfluidics has decisively shifted in recent years from the research on phenomenology and proof-of-concept fluidic functionalities to the developments oriented at applications with biology, medicine and biotechnology in prime focus. Significant efforts are made to demonstrate that microfluidics can be used ...

journal_title：Current opinion in biotechnology

authors： Scheler O,Postek W,Garstecki P

更新日期：2019-02-01 00:00:00

abstract：:Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and ...

journal_title：Current opinion in biotechnology

authors： Zargar A,Bailey CB,Haushalter RW,Eiben CB,Katz L,Keasling JD

更新日期：2017-06-01 00:00:00

abstract：:Antisense technology is finding increasing application not only in clinical development, but also for cellular engineering. Several types of antisense methods (e.g. antisense oligonucleotides, antisense RNA and small interfering RNA) can be used to inhibit the expression of a target gene. These antisense methods are b...

journal_title：Current opinion in biotechnology

authors： Lee LK,Roth CM

更新日期：2003-10-01 00:00:00

abstract：:Recent advances in research on natural products (of both low and high molecular weight) of plant origin and their commercial potential as medicines are discussed. Substances with immunomodulating, antiviral, antimicrobial, antiparasite, antitumor, anti-inflammatory, hypoglycemic, tranquillizer and antifeedant activiti...

journal_title：Current opinion in biotechnology

authors： Yamada H

更新日期：1991-04-01 00:00:00

abstract：:Recent field and laboratory studies have evaluated the potential for aerobic co-metabolism of chlorinated solvents. Different co-metabolic substrates and different methods of application have been tried, including growing indigenous microbes in situ, and injecting into the soil subsurface strains grown in subsurface r...

journal_title：Current opinion in biotechnology

authors： Semprini L

更新日期：1997-06-01 00:00:00

abstract：:Soft rot pathogens cause significant losses worldwide in fruit and vegetable production during the growing season and after harvest. These pathogens engage in molecular interactions with their plant hosts via acyl-homoserine lactone (AHL) quorum sensing, pectin metabolites, phenolic acids, and auxin. The AHL-based int...

journal_title：Current opinion in biotechnology

authors： Charkowski AO

更新日期：2009-04-01 00:00:00

abstract：:Over the review period, a significant amount of literature has been published documenting the impact of biofilms on engineered and biomedical systems. Reactor systems and analytical techniques have evolved to study the molecular chemistry and microbial ecology within biofilm layers only tens of micrometers thick, and ...

journal_title：Current opinion in biotechnology

authors： Bryers JD

更新日期：1993-04-01 00:00:00

abstract：:The conservation and sustainable use of freshwater resources is of global importance. Microorganisms are not only the most abundant organisms in natural freshwater systems, but are also key players in ecological processes controlling water quality. Detailed knowledge of the diversity and function of microorganisms dwe...

journal_title：Current opinion in biotechnology

authors： Hahn MW

更新日期：2006-06-01 00:00:00

abstract：:To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...

journal_title：Current opinion in biotechnology

authors： Azerad R,Buisson D

更新日期：2000-12-01 00:00:00

abstract：:Extremolytes are small organic molecules, which protect cells under extreme, virtually inhabitable conditions. Their exceptional properties can be translated into health-promoting and therapeutic activities, which open an avenue of opportunities for the cosmetic, medical, and food industries. Supported by powerful app...

journal_title：Current opinion in biotechnology

authors： Becker J,Wittmann C

更新日期：2020-10-01 00:00:00

abstract：:Phage display of proteins continues to be an important technology with a variety of applications. In the past year, advances have been made in coupling rational protein design with the power of the display selection process. In addition to the widely used filamentous phage, other bacteriophage surface expression syste...

journal_title：Current opinion in biotechnology

authors： Dunn IS

更新日期：1996-10-01 00:00:00

abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...

journal_title：Current opinion in biotechnology

authors： McGrath SP,Zhao FJ

更新日期：2003-06-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:The development of electrospray and matrix-assisted laser desorption mass spectrometry has provided protein chemists with tools for peptide and protein structure analysis with unprecedented sensitivity and molecular weight range. The two technologies can be viewed as competitive with respect to their molecular mass de...

journal_title：Current opinion in biotechnology

authors： Lee TD,Shively JE

更新日期：1991-02-01 00:00:00

abstract：:Marine microbes have received growing attention as sources of bioactive metabolites and offer a unique opportunity to both increase the number of marine natural products in clinical trials as well as expedite their development. This review focuses specifically on those molecules currently in the clinical pipeline that...

journal_title：Current opinion in biotechnology

authors： Waters AL,Hill RT,Place AR,Hamann MT

更新日期：2010-12-01 00:00:00

abstract：:Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...

journal_title：Current opinion in biotechnology

authors： Sittinger M,Hutmacher DW,Risbud MV

更新日期：2004-10-01 00:00:00

abstract：:Recent advances in miniaturization and automation of technologies have enabled cell-based assay high-throughput screening, bringing along new challenges in data analysis. Automation, standardization, reproducibility have become requirements for qualitative research. The Bioconductor community has worked in that direct...

journal_title：Current opinion in biotechnology

authors： Le Meur N

更新日期：2013-02-01 00:00:00

abstract：:The efficient and safe delivery of therapeutic proteins is the key to commercial success and, in some cases, the demonstration of efficacy in current and future biotechnology products. Numerous delivery technologies and companies have evolved over the past year. To critically evaluate the available options, each metho...

journal_title：Current opinion in biotechnology

authors： Cleland JL,Daugherty A,Mrsny R

更新日期：2001-04-01 00:00:00

abstract：:RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

journal_title：Current opinion in biotechnology

authors： Rossi JJ

更新日期：1992-02-01 00:00:00

abstract：:Many significant advances have occurred recently in the field of protein electrophoresis and related technologies. Improvements have been made in capillary electrophoresis in apparatus design, detection, and capillary modification with coatings and fillers. The transfer of proteins to a blot, the capacity to analyze a...

journal_title：Current opinion in biotechnology

authors： Zewert TE,Harrington MG

更新日期：1993-02-01 00:00:00

abstract：:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

journal_title：Current opinion in biotechnology

authors： Eckart MR,Bussineau CM

更新日期：1996-10-01 00:00:00

abstract：:The commercialization of high-precision microcalorimeters has led to a sharp increase in the acquisition and utilization of calorimetric data on the binding and folding reactions of proteins. This thermodynamic database, together with high-resolution structures of proteins and their complexes with ligands, has facilit...

journal_title：Current opinion in biotechnology

authors： Connelly PR

更新日期：1994-08-01 00:00:00

abstract：:The recent technological advances in next generation sequencing have brought the field closer to the goal of reconstructing all genomes within a community by presenting high throughput sequencing at much lower costs. While these next-generation sequencing technologies have allowed a massive increase in available raw s...

journal_title：Current opinion in biotechnology

authors： Scholz MB,Lo CC,Chain PS

更新日期：2012-02-01 00:00:00

abstract：:The past decade has witnessed rapid advance in microbial production of 'drop-in' biofuels from renewable resources. Various biosynthetic pathways have been constructed to produce biofuels with diverse structures, and multiple metabolic engineering strategies have been developed to increase biofuel titers, yields, prod...

journal_title：Current opinion in biotechnology

authors： Jiang W,Gu P,Zhang F

更新日期：2018-10-01 00:00:00

abstract：:Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent...

journal_title：Current opinion in biotechnology

authors： Stoloff DH,Wanunu M

更新日期：2013-08-01 00:00:00