Structural analysis of proteins by laser desorption and electrospray mass spectrometry.


:The development of electrospray and matrix-assisted laser desorption mass spectrometry has provided protein chemists with tools for peptide and protein structure analysis with unprecedented sensitivity and molecular weight range. The two technologies can be viewed as competitive with respect to their molecular mass determinations, but complementary with respect to their differences in instrumentation, sample preparation methods, and nature of spectra produced.


Curr Opin Biotechnol


Lee TD,Shively JE




Has Abstract


1991-02-01 00:00:00














  • Systems biology as a foundation for genome-scale synthetic biology.

    abstract::As the ambitions of synthetic biology approach genome-scale engineering, comprehensive characterization of cellular systems is required, as well as a means to accurately model cell-scale molecular interactions. These requirements are coincident with the goals of systems biology and, thus, systems biology will become t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Barrett CL,Kim TY,Kim HU,Palsson BØ,Lee SY

    更新日期:2006-10-01 00:00:00

  • Epigenomic technologies for deciphering circulating tumor DNA.

    abstract::Tumor-derived DNA, found in body fluids (liquid biopsy) of cancer patients as part of cell-free DNA (cfDNA), lends itself for noninvasive cancer detection and monitoring. Advantages of cfDNA as analytical target have evoked a burst of sophisticated techniques, providing clinically relevant information. Each cell type ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Daniūnaitė K,Jarmalaitė S,Kriukienė E

    更新日期:2019-02-01 00:00:00

  • Engineering carbon fixation with artificial protein organelles.

    abstract::Based on projections for global population growth, current techniques for improving agricultural yields will not be able to address future demands for major food crops. Improving photosynthetic efficiency by engineering carbon fixation has been identified as one of the most important approaches for increasing agricult...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Giessen TW,Silver PA

    更新日期:2017-08-01 00:00:00

  • Optimizing the fluorescent protein toolbox and its use.

    abstract::Fluorescent proteins (FPs) and fluorescent protein-derived biosensors are indispensable tools in life sciences. They make it possible to probe the location, activity, or interaction of molecules of interest from a subcellular to a multicellular scale. The desire for high-resolution and multidimensional information imp...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Duwé S,Dedecker P

    更新日期:2019-08-01 00:00:00

  • Bacterial biofilms.

    abstract::Over the review period, a significant amount of literature has been published documenting the impact of biofilms on engineered and biomedical systems. Reactor systems and analytical techniques have evolved to study the molecular chemistry and microbial ecology within biofilm layers only tens of micrometers thick, and ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bryers JD

    更新日期:1993-04-01 00:00:00

  • Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli.

    abstract::Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hos...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Yuzawa S,Kim W,Katz L,Keasling JD

    更新日期:2012-10-01 00:00:00

  • Static and dynamic light scattering of biological macromolecules: what can we learn?

    abstract::Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Murphy RM

    更新日期:1997-02-01 00:00:00

  • Targeted contrast agents for magnetic resonance imaging and ultrasound.

    abstract::The development of contrast agents that can be localized to a particular tissue or cellular epitope will potentially allow the noninvasive visualization and characterization of a variety of disease states. Recent advances have been made in the field of molecular imaging with magnetic resonance imaging and ultrasound a...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Morawski AM,Lanza GA,Wickline SA

    更新日期:2005-02-01 00:00:00

  • Targeting split vaccines to the endosome improves vaccination.

    abstract::Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wagner H,Heit A,Schmitz F,Bauer S

    更新日期:2004-12-01 00:00:00

  • The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology.

    abstract::Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kildegaard HF,Baycin-Hizal D,Lewis NE,Betenbaugh MJ

    更新日期:2013-12-01 00:00:00

  • Engineering peripheral nerve repair.

    abstract::Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Marquardt LM,Sakiyama-Elbert SE

    更新日期:2013-10-01 00:00:00

  • Silent constraints: the hidden challenges faced in plant metabolic engineering.

    abstract::Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lynch JH,Huang XQ,Dudareva N

    更新日期:2021-01-08 00:00:00

  • Growth factors as wound healing agents.

    abstract::Wound healing can no longer be considered a generic term: it is the summation of a complex series of processes beginning with coagulation and ending in remodelling. This review, therefore, summarizes the past year's advances in growth factor research according to the roles of the individual growth factors in the speci...

    journal_title:Current opinion in biotechnology

    pub_type: 临床试验,杂志文章,评审


    authors: Robson MC

    更新日期:1991-12-01 00:00:00

  • Protein processing within the secretory pathway.

    abstract::Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rehemtulla A,Kaufman RJ

    更新日期:1992-10-01 00:00:00

  • Novel applications of the ubiquitin-dependent proteolytic pathway in plant genetic engineering.

    abstract::One goal of plant genetic engineering is the manipulation of protein levels within crop plants. New insights into the ubiquitin-dependent proteolytic pathway provide potential novel ways of enhancing levels of desired proteins by synthesizing them as ubiquitin fusions, and reducing levels of undesired proteins by sele...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hondred D,Vierstra RD

    更新日期:1992-04-01 00:00:00

  • Isotopes in geobiochemistry: tracing metabolic pathways in microorganisms of environmental relevance with stable isotopes.

    abstract::Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connec...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Adrian L,Marco-Urrea E

    更新日期:2016-10-01 00:00:00

  • Adaptive laboratory evolution--harnessing the power of biology for metabolic engineering.

    abstract::Adaptive laboratory evolution (ALE) strategies allow for the metabolic engineering of microorganisms by combining genetic variation with the selection of beneficial mutations in an unbiased fashion. These ALE strategies have been proven highly effective in the optimization of production strains. In contrast to rationa...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Portnoy VA,Bezdan D,Zengler K

    更新日期:2011-08-01 00:00:00

  • RNA as a drug target: chemical, modelling, and evolutionary tools.

    abstract::Dramatic technical progress in RNA synthesis and structure determination has allowed several difficulties inherent to the preparation, handling and structural analysis of RNA to be overcome, and this has led to a wealth of information about RNA structure and its relationship with biological function. It is now fully r...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hermann T,Westhof E

    更新日期:1998-02-01 00:00:00

  • Plant GSTome: structure and functional role in xenome network and plant stress response.

    abstract::Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Labrou NE,Papageorgiou AC,Pavli O,Flemetakis E

    更新日期:2015-04-01 00:00:00

  • Biological approaches for controlling shellfish-associated pathogens.

    abstract::As the consumption of seafood and shellfish increases around the world, so is the incidence of associated outbreaks of illness. Various postharvest treatments are effective at killing seafood-associated bacteria, but most of these treatments also kill the mollusks. Because consumer preferences for raw live shellfish p...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Teplitski M,Wright AC,Lorca G

    更新日期:2009-04-01 00:00:00

  • Engineering nanoreactors using bacterial microcompartment architectures.

    abstract::Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of B...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Plegaria JS,Kerfeld CA

    更新日期:2018-06-01 00:00:00

  • Microorganisms relevant to bioremediation.

    abstract::Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Watanabe K

    更新日期:2001-06-01 00:00:00

  • Computational methods for evaluation of cell-based data assessment--Bioconductor.

    abstract::Recent advances in miniaturization and automation of technologies have enabled cell-based assay high-throughput screening, bringing along new challenges in data analysis. Automation, standardization, reproducibility have become requirements for qualitative research. The Bioconductor community has worked in that direct...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Le Meur N

    更新日期:2013-02-01 00:00:00

  • Customised nucleic acid libraries for enhanced aptamer selection and performance.

    abstract::Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applicat...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pfeiffer F,Rosenthal M,Siegl J,Ewers J,Mayer G

    更新日期:2017-12-01 00:00:00

  • The use of adenoviral vectors for gene therapy and gene transfer in vivo.

    abstract::Adenoviral vectors have proven to be excellent vehicles for gene delivery in vivo to a wide range of cell types. These vectors have been used to transfer genes such as CFTR to correct the defect in cystic fibrosis and, more recently, to supply serum blood factors and genetically modify tumors to enhance therapy. ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bramson JL,Graham FL,Gauldie J

    更新日期:1995-10-01 00:00:00

  • Biocatalytic synthesis of oligosaccharides.

    abstract::Tremendous advances in biocatalytic approaches to oligosaccharide synthesis have taken place in the past two years. The use of isolated enzymes, both glycosyltransferases and glycosidases, or engineered whole cells allows the preparation of natural oligosaccharides and analogs required for glycobiology research. ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Palcic MM

    更新日期:1999-12-01 00:00:00

  • Biocatalytic synthesis of hydroxylated natural products using aldolases and related enzymes.

    abstract::Synthetic building blocks bearing hydroxylated chiral centers are important targets for biocatalysis. Many C-C bond forming enzymes have recently been investigated for new applications and new strategies towards the synthesis of natural products and related oxygenated compounds. Several old catalysts have been studied...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Fessner WD,Helaine V

    更新日期:2001-12-01 00:00:00

  • Rapid, sensitive sequencing of oligosaccharides from glycoproteins.

    abstract::The release of sub-picomole levels of N-linked oligosaccharides directly from 1-5 micrograms of protein in a band on an SDS PAGE gel, coupled with recent advances in mass spectrometry and HPLC, opens the way for the analysis of biologically important glycoproteins that are difficult to purify or are available only in ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rudd PM,Dwek RA

    更新日期:1997-08-01 00:00:00

  • Single cell stable isotope probing in microbiology using Raman microspectroscopy.

    abstract::Microbial communities are essential for most ecosystem processes and interact in highly complex ways with virtually all eukaryotes. Thus, a detailed understanding of the function of such communities is a fundamental prerequisite for microbial ecologists, applied microbiologists and microbiome researchers. Using single...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wang Y,Huang WE,Cui L,Wagner M

    更新日期:2016-10-01 00:00:00

  • Transcription factor-based biosensors and inducible systems in non-model bacteria: current progress and future directions.

    abstract::Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kim NM,Sinnott RW,Sandoval NR

    更新日期:2020-08-01 00:00:00