Engineering of silk proteins for materials applications.

abstract：:Plant-based foods offer a wide range of nutrients that are essential for human and animal health. Among these nutrients, iron stands out as one of the most important micronutrients. Increasing the iron content in many staple and non-staple plant foods continues to be a goal of many scientists around the world. However...

journal_title：Current opinion in biotechnology

authors： Vasconcelos MW,Gruissem W,Bhullar NK

更新日期：2017-04-01 00:00:00

abstract：:Synthetic proteins provide important information about the principles of protein structure. They illuminate the processes of natural protein evaluation, but are not limited by these processes. Here, we review studies of several mutant proteins and discuss the general principles that can be derived from them. ...

journal_title：Current opinion in biotechnology

authors： Pastore A,Lesk AM

更新日期：1991-08-01 00:00:00

abstract：:Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...

journal_title：Current opinion in biotechnology

authors： Murphy RM

更新日期：1997-02-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:The federal government is critically examining its responsibilities and opportunities for bringing the new field of gene therapy to fruition and for assuring public confidence in this new area of biomedicine. The evolving mechanisms for review and regulation in human gene transfer studies in the United States are bein...

journal_title：Current opinion in biotechnology

authors： Friedmann T,Noguchi P,Mickelson C

更新日期：2001-06-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00

abstract：:Life depends on the interaction of proteins. The availability of the complete human genome sequence has highlighted the need for a tool to analyse protein interactions and several databases have been compiled for this purpose. These databases document, categorize, and analyze interacting proteins and the cellular func...

journal_title：Current opinion in biotechnology

authors： Xenarios I,Eisenberg D

更新日期：2001-08-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...

journal_title：Current opinion in biotechnology

authors： Schwager J,Mohajeri MH,Fowler A,Weber P

更新日期：2008-04-01 00:00:00

abstract：:Recent advances in miniaturization and automation of technologies have enabled cell-based assay high-throughput screening, bringing along new challenges in data analysis. Automation, standardization, reproducibility have become requirements for qualitative research. The Bioconductor community has worked in that direct...

journal_title：Current opinion in biotechnology

authors： Le Meur N

更新日期：2013-02-01 00:00:00

abstract：:Oil prices and government mandates have catalyzed rapid growth of nonfossil transportation fuels in recent years, with a large focus on ethanol from energy crops, but the food crops used as first-generation energy crops today are not optimized for this purpose. We show that the theoretical efficiency of conversion of ...

journal_title：Current opinion in biotechnology

authors： Heaton EA,Flavell RB,Mascia PN,Thomas SR,Dohleman FG,Long SP

更新日期：2008-06-01 00:00:00

abstract：:Isothermal titration calorimetry is widely used to measure the affinities and enthalpies of interaction between proteins and/or small molecules. The quantitative nature of the technique is especially useful in the characterization of recombinant proteins while determining the fraction of protein capable of binding a s...

journal_title：Current opinion in biotechnology

authors： Baranauskiene L,Kuo TC,Chen WY,Matulis D

更新日期：2019-02-01 00:00:00

abstract：:Synthetic building blocks bearing hydroxylated chiral centers are important targets for biocatalysis. Many C-C bond forming enzymes have recently been investigated for new applications and new strategies towards the synthesis of natural products and related oxygenated compounds. Several old catalysts have been studied...

journal_title：Current opinion in biotechnology

authors： Fessner WD,Helaine V

更新日期：2001-12-01 00:00:00

abstract：:The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medi...

journal_title：Current opinion in biotechnology

authors： Schwechheimer SK,Becker J,Wittmann C

更新日期：2018-12-01 00:00:00

abstract：:The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...

journal_title：Current opinion in biotechnology

authors： Krell T,Lacal J,Reyes-Darias JA,Jimenez-Sanchez C,Sungthong R,Ortega-Calvo JJ

更新日期：2013-06-01 00:00:00

abstract：:In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these display techniques ha...

journal_title：Current opinion in biotechnology

authors： Amstutz P,Forrer P,Zahnd C,Plückthun A

更新日期：2001-08-01 00:00:00

abstract：:Heterotrophic bacteria are an integral part of any environmental biotechnology process (EBP). Therefore, factors controlling bacterial abundance, activity, and community composition are central to the understanding of such processes. Among these factors, top-down control by bacteriophage predation has so far received ...

journal_title：Current opinion in biotechnology

authors： Shapiro OH,Kushmaro A

更新日期：2011-06-01 00:00:00

abstract：:Enormous fuel consumption and growing environmental concerns have been spurring development of renewable biofuels. Among various biofuels, fatty acid-derived biohydrocarbons are ideal alternatives to non-renewable fossil fuels due to their closest properties to petroleum-based fuels. In the past decade, novel hydrocar...

journal_title：Current opinion in biotechnology

authors： Liu K,Li S

更新日期：2020-04-01 00:00:00

abstract：:The concept of whole genome amplification is something that has arisen in the past few years as the polymerase chain reaction (PCR) has been adapted to replicate regions of genomes that are of biological interest. The applications are many--forensic science, embryonic disease diagnosis, bioterrorism genome detection, ...

journal_title：Current opinion in biotechnology

authors： Hawkins TL,Detter JC,Richardson PM

更新日期：2002-02-01 00:00:00

abstract：:Fermentation of syngas is a means through which unutilized organic waste streams can be converted biologically into biofuels and commodity chemicals. Despite recent advances, several issues remain which limit implementation of industrial-scale syngas fermentation processes. At the cellular level, the energy conservati...

journal_title：Current opinion in biotechnology

authors： Latif H,Zeidan AA,Nielsen AT,Zengler K

更新日期：2014-06-01 00:00:00

abstract：:In recent years a number of aglycosylated therapeutic antibodies have entered the clinic. The clinical evaluation of these antibodies has served to dispel concerns that the absence of the ubiquitous N297 glycan in the Fc of IgG might result in immunogenicity, poor in vivo stability or unfavorable pharmacokinetics. Imp...

journal_title：Current opinion in biotechnology

authors： Jung ST,Kang TH,Kelton W,Georgiou G

更新日期：2011-12-01 00:00:00

abstract：:With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

journal_title：Current opinion in biotechnology

authors： Kontermann RE

更新日期：2011-12-01 00:00:00

abstract：:Metabolomics consists of strategies to quantitatively identify cellular metabolites and to understand how trafficking of these biochemical messengers through the metabolic network influences phenotype. The application of metabolomics to fungi has been strongly pursued because these organisms are widely used for the pr...

journal_title：Current opinion in biotechnology

authors： Jewett MC,Hofmann G,Nielsen J

更新日期：2006-04-01 00:00:00

abstract：:Ultrasonographic imaging of ovarian morphology is used widely to inform reproductive health status in women. Metabolic disturbances induced by a negative energy balance (e.g. undernutrition) or positive energy balance (e.g. overnutrition, obesity) are known to drive or exacerbate reproductive dysfunction. Whether the ...

journal_title：Current opinion in biotechnology

authors： Vanden Brink H,Pea J,Lujan ME

更新日期：2020-11-25 00:00:00

abstract：:Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UB...

journal_title：Current opinion in biotechnology

authors： Lee KH,Hamashima K,Kimoto M,Hirao I

更新日期：2018-06-01 00:00:00

abstract：:To mitigate some of the potentially deleterious environmental and agricultural consequences associated with current land-based-biofuel feedstocks, we propose the use of biofuels derived from aquatic microbial oxygenic photoautotrophs (AMOPs), more commonly known as cyanobacteria, algae, and diatoms. Herein we review t...

journal_title：Current opinion in biotechnology

authors： Dismukes GC,Carrieri D,Bennette N,Ananyev GM,Posewitz MC

更新日期：2008-06-01 00:00:00

abstract：:Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...

journal_title：Current opinion in biotechnology

authors： Liu Y,Nielsen J

更新日期：2019-12-01 00:00:00

abstract：:Extremolytes are small organic molecules, which protect cells under extreme, virtually inhabitable conditions. Their exceptional properties can be translated into health-promoting and therapeutic activities, which open an avenue of opportunities for the cosmetic, medical, and food industries. Supported by powerful app...

journal_title：Current opinion in biotechnology

authors： Becker J,Wittmann C

更新日期：2020-10-01 00:00:00

abstract：:Organ-on-chip systems integrate microfluidic technology and living cells to study human physiology and pathophysiology. These human in vitro models are promising substitutes for animal testing, and their small scale enables precise control of culture conditions and high-throughput experiments, which would not be econo...

journal_title：Current opinion in biotechnology

authors： Luni C,Serena E,Elvassore N

更新日期：2014-02-01 00:00:00

abstract：:The use of novel materials in the nano-scale size range for applications in devices, drugs and diagnostic agents comes with a number of new opportunities, and also serious challenges to human applications. The larger size of particulate-based agents, as compared to traditional drugs, allows for the significant advanta...

journal_title：Current opinion in biotechnology

authors： Scheinberg DA,Grimm J,Heller DA,Stater EP,Bradbury M,McDevitt MR

更新日期：2017-08-01 00:00:00