Human-on-chip for therapy development and fundamental science.

abstract：:In the past two decades, biological nanopores have been developed and explored for use in sensing applications as a result of their exquisite sensitivity and easily engineered, reproducible, and economically manufactured structures. Nanopore sensing has been shown to differentiate between highly similar analytes, meas...

journal_title：Current opinion in biotechnology

authors： Schmidt J

更新日期：2016-06-01 00:00:00

abstract：:Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...

journal_title：Current opinion in biotechnology

authors： Murphy RM

更新日期：1997-02-01 00:00:00

abstract：:Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead...

journal_title：Current opinion in biotechnology

authors： Carrillo-Carrion C,Carril M,Parak WJ

更新日期：2017-08-01 00:00:00

abstract：:Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

journal_title：Current opinion in biotechnology

authors： Friedrich MW

更新日期：2006-02-01 00:00:00

abstract：:Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

journal_title：Current opinion in biotechnology

authors： Tissier A

更新日期：2018-02-01 00:00:00

abstract：:Poxviruses are widely used for the cytoplasmic expression of recombinant genes in mammalian cells. Recent improvements allow high expression and simplify the integration of multiple foreign genes. Vaccinia virus mutants and other poxviruses that undergo abortive infection in mammalian cells are receiving special atten...

journal_title：Current opinion in biotechnology

authors： Carroll MW,Moss B

更新日期：1997-10-01 00:00:00

abstract：:Synthetic building blocks bearing hydroxylated chiral centers are important targets for biocatalysis. Many C-C bond forming enzymes have recently been investigated for new applications and new strategies towards the synthesis of natural products and related oxygenated compounds. Several old catalysts have been studied...

journal_title：Current opinion in biotechnology

authors： Fessner WD,Helaine V

更新日期：2001-12-01 00:00:00

abstract：:Although metabolic networks can be readily reconstructed through comparative genomics, the reconstruction of regulatory networks has been hindered by the relatively low level of evolutionary conservation of their molecular components. Recent developments in experimental techniques have allowed the generation of vast a...

journal_title：Current opinion in biotechnology

authors： Herrgård MJ,Covert MW,Palsson BØ

更新日期：2004-02-01 00:00:00

abstract：:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

journal_title：Current opinion in biotechnology

authors： Rashtchian A

更新日期：1995-02-01 00:00:00

abstract：:Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological conditions. Abs are required for the quantification of targets, dete...

journal_title：Current opinion in biotechnology

authors： Basu K,Green EM,Cheng Y,Craik CS

更新日期：2019-12-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...

journal_title：Current opinion in biotechnology

authors： Kildegaard HF,Baycin-Hizal D,Lewis NE,Betenbaugh MJ

更新日期：2013-12-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00

abstract：:The structure determinations of the cytochrome bc1 complex and the prokaryotic potassium channel demonstrate that a wider range of membrane proteins are now amenable to study by X-ray crystallography. Furthermore, the structures of porins and interfacial membrane proteins show that membrane structural biology is becom...

journal_title：Current opinion in biotechnology

authors： Garavito RM

更新日期：1998-08-01 00:00:00

abstract：:Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nu...

journal_title：Current opinion in biotechnology

authors： Houlihan G,Arangundy-Franklin S,Holliger P

更新日期：2017-12-01 00:00:00

abstract：:Microbial communities associated with a plant host, constituting a holobiont, affect the physiology and growth of the plant via metabolites that are mainly derived from their photosynthates. The structure and function of active microbial communities that assimilate root exudates can be tracked by using stable isotope ...

journal_title：Current opinion in biotechnology

authors： Haichar FEZ,Heulin T,Guyonnet JP,Achouak W

更新日期：2016-10-01 00:00:00

abstract：:There is growing scientific evidence supported by mechanistic and clinical studies that probiotics can provide health benefits. As probiotics are highly sensitive to many environmental factors, and because the propagation of many strains of intestinal origin is not straightforward, most commercial strains are selected...

journal_title：Current opinion in biotechnology

authors： Lacroix C,Yildirim S

更新日期：2007-04-01 00:00:00

abstract：:The biosynthesis of the solvents 1-butanol and acetone is restricted to species of the genus Clostridium, a diverse group of Gram-positive, endospore forming anaerobes comprising toxin-producing strains as well as terrestrial non-pathogenic species of biotechnological impact. Among solventogenic clostridia, Clostridiu...

journal_title：Current opinion in biotechnology

authors： Lütke-Eversloh T,Bahl H

更新日期：2011-10-01 00:00:00

abstract：:Biomaterials are increasingly being developed as in vitro microenvironments mimicking in vivo stem cell niches. However, current macroscale methodologies to produce these niche models fail to recapitulate the spatial and temporal characteristics of the complex native stem cell regulatory systems. Microfluidic technolo...

journal_title：Current opinion in biotechnology

authors： Kobel S,Lutolf MP

更新日期：2011-10-01 00:00:00

abstract：:In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

journal_title：Current opinion in biotechnology

authors： Gumpert J,Hoischen C

更新日期：1998-10-01 00:00:00

abstract：:The versatile functionality of microbial biocatalysts offers a promising solution to the growing need to replace conventional, petroleum-derived fuels, chemicals, and materials with sustainable alternatives from renewable biomass. Whereas metabolic pathway engineering and strain optimization have greatly expanded the ...

journal_title：Current opinion in biotechnology

authors： Thompson B,Moon TS,Nielsen DR

更新日期：2014-12-01 00:00:00

abstract：:Microbes have long been adapted for the biosynthetic production of useful compounds. There is increasing demand for the rapid and cheap microbial production of diverse molecules in an industrial setting. Microbes can now be designed and engineered for a particular biosynthetic purpose, thanks to recent developments in...

journal_title：Current opinion in biotechnology

authors： Quin MB,Schmidt-Dannert C

更新日期：2014-10-01 00:00:00

abstract：:Since the development of desorption electrospray ionization (DESI), many other ionization methods for ambient and atmospheric pressure mass spectrometry have been developed. Ambient ionization mass spectrometry has now been used for a wide variety of biological applications, including plant science, microbiology, neur...

journal_title：Current opinion in biotechnology

authors： Hsu CC,Dorrestein PC

更新日期：2015-02-01 00:00:00

abstract：:Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

journal_title：Current opinion in biotechnology

authors： Kim NM,Sinnott RW,Sandoval NR

更新日期：2020-08-01 00:00:00

abstract：:The concept of whole genome amplification is something that has arisen in the past few years as the polymerase chain reaction (PCR) has been adapted to replicate regions of genomes that are of biological interest. The applications are many--forensic science, embryonic disease diagnosis, bioterrorism genome detection, ...

journal_title：Current opinion in biotechnology

authors： Hawkins TL,Detter JC,Richardson PM

更新日期：2002-02-01 00:00:00

abstract：:Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically...

journal_title：Current opinion in biotechnology

authors： Prangemeier T,Lehr FX,Schoeman RM,Koeppl H

更新日期：2020-06-01 00:00:00

abstract：:Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

journal_title：Current opinion in biotechnology

authors： Meyer RD,Cook D

更新日期：2000-02-01 00:00:00

abstract：:Xenopus oocytes have been used as a surrogate genetic system for the study of many facets of gene expression. Some recent salient examples where injected oocytes proved to be indispensable for the analysis of transcription, translation, RNA/protein transport, and ion channel formation are described. ...

journal_title：Current opinion in biotechnology

authors： Richter JD

更新日期：1991-10-01 00:00:00

abstract：:Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated biochemical reactions by in vitro assembling a number of enzymes or their complexes and coenzymes. Assembly of numerous enzymes without cellular membrane, gene regulation, or undesired pathway can circumvent some of the obstacle...

journal_title：Current opinion in biotechnology

authors： Zhang YH,Sun J,Zhong JJ

更新日期：2010-10-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activiti...

journal_title：Current opinion in biotechnology

authors： Allen DK,Young JD

更新日期：2020-08-01 00:00:00