Single cell stable isotope probing in microbiology using Raman microspectroscopy.

abstract：:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

journal_title：Current opinion in biotechnology

authors： Rashtchian A

更新日期：1995-02-01 00:00:00

abstract：:Wound healing can no longer be considered a generic term: it is the summation of a complex series of processes beginning with coagulation and ending in remodelling. This review, therefore, summarizes the past year's advances in growth factor research according to the roles of the individual growth factors in the speci...

journal_title：Current opinion in biotechnology

authors： Robson MC

更新日期：1991-12-01 00:00:00

abstract：:The world continues to increase its energy use, brought about by an expanding population and a desire for a greater standard of living. This energy use coupled with the realization of the impact of carbon dioxide on the climate, has led us to reanalyze the potential of plant-based biofuels. Of the potential sources of...

journal_title：Current opinion in biotechnology

authors： Jones CS,Mayfield SP

更新日期：2012-06-01 00:00:00

abstract：:With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

journal_title：Current opinion in biotechnology

authors： Karst DJ,Steinebach F,Morbidelli M

更新日期：2018-10-01 00:00:00

abstract：:Characterization of the various microbial populations present in exoelectrogenic biofilms provides insight into the processes required to convert complex organic matter in wastewater streams into electrical current in bioelectrochemical systems (BESs). Analysis of the community profiles of exoelectrogenic microbial co...

journal_title：Current opinion in biotechnology

authors： Kiely PD,Regan JM,Logan BE

更新日期：2011-06-01 00:00:00

abstract：:Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an...

journal_title：Current opinion in biotechnology

authors： Bock R

更新日期：2007-04-01 00:00:00

abstract：:Strain-specificity of probiotic effects has been a cornerstone principle of probiotic science for decades. Certainly, some important mechanisms are present in only a few probiotic strains. But scientific advances now reveal commonalities among members of certain taxonomic groups of probiotic microbes. Some clinical be...

journal_title：Current opinion in biotechnology

authors： Sanders ME,Benson A,Lebeer S,Merenstein DJ,Klaenhammer TR

更新日期：2018-02-01 00:00:00

abstract：:Obtaining an abundant supply of human cells with specific differentiated properties is a key issue in many research studies and biotechnology applications. Recent advances in this area include the finding that forced expression of the enzyme telomerase can greatly increase the proliferative potential of at least some ...

journal_title：Current opinion in biotechnology

authors： Yeager TR,Reddel RR

更新日期：1999-10-01 00:00:00

abstract：:Patient-derived xenografts (PDXs) are obtained by transplanting fragments of a patient's tumour into immunodeficient mice. Growth and propagation of PDXs allows correlating therapeutic response in vivo with extensive, multi-dimensional molecular annotation, leading to identification of predictive biomarkers. PDXs are ...

journal_title：Current opinion in biotechnology

authors： Invrea F,Rovito R,Torchiaro E,Petti C,Isella C,Medico E

更新日期：2020-06-01 00:00:00

abstract：:Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...

journal_title：Current opinion in biotechnology

authors： Lee PS,Lee KH

更新日期：2000-04-01 00:00:00

abstract：:Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies...

journal_title：Current opinion in biotechnology

authors： Suter B,Kittanakom S,Stagljar I

更新日期：2008-08-01 00:00:00

abstract：:Vernalization is the process by which flowering is promoted by prolonged exposure to the cold of a typical winter. In certain plant species, the role of vernalization is to suppress the expression of genes that encode repressors of flowering. In Arabidopsis, this suppression is an epigenetic phenomenon in the sense th...

journal_title：Current opinion in biotechnology

authors： Amasino RM

更新日期：2005-04-01 00:00:00

abstract：:Anaerobic digestion is the default process for biological conversion of residue organics to renewable energy and biofuel in the form of methane. However, its scope of application is expanding, due to availability of new technologies, and the emerging drivers of energy and nutrient conservation and recovery. Here, we o...

journal_title：Current opinion in biotechnology

authors： Batstone DJ,Virdis B

更新日期：2014-06-01 00:00:00

abstract：:Biotechnology applications require engineering complex multi-genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole bio...

journal_title：Current opinion in biotechnology

authors： Bassalo MC,Liu R,Gill RT

更新日期：2016-06-01 00:00:00

abstract：:Recent advances in emerging food-processing technologies, such as high hydrostatic pressure or high-intensity electric field pulses, allow targeted and sophisticated modification and preservation of foods. We are beginning to understand the mechanisms involved in pressure inactivation of bacterial spores and have been...

journal_title：Current opinion in biotechnology

authors： Knorr D

更新日期：1999-10-01 00:00:00

abstract：:Fifteen years have passed since T-cell receptor (TCR) genes were identified (reviewed in [1]). Unlike the situation for antibodies, no direct structural information on the TCR proteins has been available for most of this time. Recently, however, the crystal structures of isolated alpha and beta chains were determined,...

journal_title：Current opinion in biotechnology

authors： Garcia KC,Teyton L

更新日期：1998-08-01 00:00:00

abstract：:The gastrointestinal (GI) tract is a complex system characterized by multiple cell types with a determined architectural arrangement. Tissue engineering of the GI tract aims to reinstate the architecture and function of all structural layers. The key point for successful tissue regeneration includes the use of cells/b...

journal_title：Current opinion in biotechnology

authors： Bitar KN,Zakhem E

更新日期：2013-10-01 00:00:00

abstract：:Adverse environmental conditions pose serious limitations to agricultural production. Classical biotechnological approaches towards increasing abiotic stress tolerance focus on boosting plant endogenous defence mechanisms. However, overexpression of regulatory elements or effectors is usually accompanied by growth han...

journal_title：Current opinion in biotechnology

authors： Cabello JV,Lodeyro AF,Zurbriggen MD

更新日期：2014-04-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...

journal_title：Current opinion in biotechnology

authors： Liu Y,Nielsen J

更新日期：2019-12-01 00:00:00

abstract：:With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

journal_title：Current opinion in biotechnology

authors： Kontermann RE

更新日期：2011-12-01 00:00:00

abstract：:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the p...

journal_title：Current opinion in biotechnology

authors： Gygi SP,Rist B,Aebersold R

更新日期：2000-08-01 00:00:00

abstract：:Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of B...

journal_title：Current opinion in biotechnology

authors： Plegaria JS,Kerfeld CA

更新日期：2018-06-01 00:00:00

abstract：:Transdifferentiation of one cell type to another has garnered significant research efforts in recent years. As cardiomyocyte loss following myocardial infarction becomes debilitating for cardiac patients, the option of an autologous source of cardiomyocytes not derived from multi/pluripotent stem cell sources is an at...

journal_title：Current opinion in biotechnology

authors： Monaghan MG,Holeiter M,Layland SL,Schenke-Layland K

更新日期：2016-08-01 00:00:00

abstract：:The recent adoption of whole plasmid genome sequencing as a routine analytical technique has provided the basis for cataloging the historical events through which plasmids are assembled from the available families of modular plasmid components. Horizontal gene transfer mediated by plasmids plays an important role in t...

journal_title：Current opinion in biotechnology

authors： Dennis JJ

更新日期：2005-06-01 00:00:00

abstract：:Plant biotechnology has been around since the advent of humankind, resulting in tremendous improvements in plant cultivation through crop domestication, breeding and selection. The emergence of transgenic approaches involving the introduction of defined DNA sequences into plants by humans has rapidly changed the surfa...

journal_title：Current opinion in biotechnology

authors： Francis D,Finer JJ,Grotewold E

更新日期：2017-04-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:New microbial tools enable detailed quantification and characterization of complex drinking water (DW) microbiomes. Many opportunities exist from source to tap to apply this knowledge toward management of the microbiology. This requires consideration of the microbiome continuum across all phases harboring microbes (pl...

journal_title：Current opinion in biotechnology

authors： Proctor CR,Hammes F

更新日期：2015-06-01 00:00:00

abstract：:Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

journal_title：Current opinion in biotechnology

authors： Watanabe K

更新日期：2001-06-01 00:00:00

abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...

journal_title：Current opinion in biotechnology

authors： Schwager J,Mohajeri MH,Fowler A,Weber P

更新日期：2008-04-01 00:00:00