Genomic analysis.

abstract：:The most significant feature of the current transgenic models of Alzheimer's disease continues to be the amyloid phenotype. In the past year, mice have been more extensively characterized in terms of the effect of amyloid accumulation on downstream events, such as neurodegeneration and behavioral changes, but the resu...

journal_title：Current opinion in biotechnology

authors： Duff K

更新日期：1998-12-01 00:00:00

abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...

journal_title：Current opinion in biotechnology

authors： O'Malley J,Woltjen K,Kaji K

更新日期：2009-10-01 00:00:00

abstract：:The pathway of monolignol biosynthesis involves many components interacting in a metabolic grid to regulate the supply and ratios of monolignols for lignification. The complexity of the pathway challenges any intuitive prediction of the output without mathematical modeling. Several models have been presented to quanti...

journal_title：Current opinion in biotechnology

authors： Wang JP,Matthews ML,Naik PP,Williams CM,Ducoste JJ,Sederoff RR,Chiang VL

更新日期：2019-04-01 00:00:00

abstract：:Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead...

journal_title：Current opinion in biotechnology

authors： Carrillo-Carrion C,Carril M,Parak WJ

更新日期：2017-08-01 00:00:00

abstract：:Much of the current knowledge on the hydrothermic liquefaction of biomass to biocrude is on the basis of laboratory benchtop findings, and the step up to industrial scale reactors will require a range of information that is currently either unavailable or insufficient. This work highlights a number of these issues suc...

journal_title：Current opinion in biotechnology

authors： Lee A,Lewis D,Kalaitzidis T,Ashman P

更新日期：2016-04-01 00:00:00

abstract：:The biosynthesis of the solvents 1-butanol and acetone is restricted to species of the genus Clostridium, a diverse group of Gram-positive, endospore forming anaerobes comprising toxin-producing strains as well as terrestrial non-pathogenic species of biotechnological impact. Among solventogenic clostridia, Clostridiu...

journal_title：Current opinion in biotechnology

authors： Lütke-Eversloh T,Bahl H

更新日期：2011-10-01 00:00:00

abstract：:The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...

journal_title：Current opinion in biotechnology

authors： Krell T,Lacal J,Reyes-Darias JA,Jimenez-Sanchez C,Sungthong R,Ortega-Calvo JJ

更新日期：2013-06-01 00:00:00

abstract：:Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...

journal_title：Current opinion in biotechnology

authors： Hazen TC,Rocha AM,Techtmann SM

更新日期：2013-06-01 00:00:00

abstract：:Reductive catalytic fractionation (RCF) of lignocellulose is an emerging biorefinery scheme that combines biomass fractionation with lignin depolymerisation. Central to this scheme is the integration of heterogeneous catalysis, which overcomes the tendency of lignin to repolymerise. Ultimately, this leads to a low-Mw ...

journal_title：Current opinion in biotechnology

authors： Renders T,Van den Bossche G,Vangeel T,Van Aelst K,Sels B

更新日期：2019-04-01 00:00:00

abstract：:Phage display of proteins continues to be an important technology with a variety of applications. In the past year, advances have been made in coupling rational protein design with the power of the display selection process. In addition to the widely used filamentous phage, other bacteriophage surface expression syste...

journal_title：Current opinion in biotechnology

authors： Dunn IS

更新日期：1996-10-01 00:00:00

abstract：:Combinatorial biocatalysis is an emerging technology in the field of drug discovery. The biocatalytic approach to combinatorial chemistry uses enzymatic, chemoenzymatic, and microbial transformations to generate libraries from lead compounds. Important recent advances in combinatorial biocatalysis include iterative de...

journal_title：Current opinion in biotechnology

authors： Altreuter DH,Clark DS

更新日期：1999-04-01 00:00:00

abstract：:Saccharomyces cerevisiae has decisive advantages in industrial processes due to its tolerance to alcohols and fermentation conditions. Butanol isomers are considered as suitable fuel substitutes and valuable biomass-derived chemical building blocks. Whereas high production was achieved with bacterial systems, metaboli...

journal_title：Current opinion in biotechnology

authors： Generoso WC,Schadeweg V,Oreb M,Boles E

更新日期：2015-06-01 00:00:00

abstract：:In recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. It has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ESTs had limited impact for breeding. Until recently, the models used by ...

journal_title：Current opinion in biotechnology

authors： Dal-Bianco M,Carneiro MS,Hotta CT,Chapola RG,Hoffmann HP,Garcia AA,Souza GM

更新日期：2012-04-01 00:00:00

abstract：:Tumor-derived DNA, found in body fluids (liquid biopsy) of cancer patients as part of cell-free DNA (cfDNA), lends itself for noninvasive cancer detection and monitoring. Advantages of cfDNA as analytical target have evoked a burst of sophisticated techniques, providing clinically relevant information. Each cell type ...

journal_title：Current opinion in biotechnology

authors： Daniūnaitė K,Jarmalaitė S,Kriukienė E

更新日期：2019-02-01 00:00:00

abstract：:Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

journal_title：Current opinion in biotechnology

authors： Kim NM,Sinnott RW,Sandoval NR

更新日期：2020-08-01 00:00:00

abstract：:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for preci...

journal_title：Current opinion in biotechnology

authors： Tolle F,Stücheli P,Fussenegger M

更新日期：2019-10-01 00:00:00

abstract：:Thiopeptide antibiotics, which are a class of sulfur-rich and highly modified peptide natural products, exhibit a wide variety of important biological properties. These antibiotics are ribosomally synthesized and arise from post-translational modifications, exemplifying a process through which nature develops the stru...

journal_title：Current opinion in biotechnology

authors： Lin Z,He Q,Liu W

更新日期：2017-12-01 00:00:00

abstract：:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...

journal_title：Current opinion in biotechnology

authors： Vervoort Y,Linares AG,Roncoroni M,Liu C,Steensels J,Verstrepen KJ

更新日期：2017-08-01 00:00:00

abstract：:Strain-specificity of probiotic effects has been a cornerstone principle of probiotic science for decades. Certainly, some important mechanisms are present in only a few probiotic strains. But scientific advances now reveal commonalities among members of certain taxonomic groups of probiotic microbes. Some clinical be...

journal_title：Current opinion in biotechnology

authors： Sanders ME,Benson A,Lebeer S,Merenstein DJ,Klaenhammer TR

更新日期：2018-02-01 00:00:00

abstract：:Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically...

journal_title：Current opinion in biotechnology

authors： Prangemeier T,Lehr FX,Schoeman RM,Koeppl H

更新日期：2020-06-01 00:00:00

abstract：:Gene expression control is critical to increase production of recombinant proteins, fine-tune metabolic pathways and reliably express synthetic pathways. The importance of transcriptional control seems to be most important in eukaryotic systems. In this review, we highlight recent developments in the field of transcri...

journal_title：Current opinion in biotechnology

authors： Leavitt JM,Alper HS

更新日期：2015-08-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:A growing body of evidence indicates that many cellular reactions within metabolic pathways are catalyzed not by free-floating 'soluble' enzymes, but via one or more membrane-associated multienzyme complexes. This type of macromolecular organization has important implications for the overall efficiency, specificity, a...

journal_title：Current opinion in biotechnology

authors： Conrado RJ,Varner JD,DeLisa MP

更新日期：2008-10-01 00:00:00

abstract：:With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

journal_title：Current opinion in biotechnology

authors： Kontermann RE

更新日期：2011-12-01 00:00:00

abstract：:Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

journal_title：Current opinion in biotechnology

authors： Wagner H,Heit A,Schmitz F,Bauer S

更新日期：2004-12-01 00:00:00

abstract：:Over the review period, a significant amount of literature has been published documenting the impact of biofilms on engineered and biomedical systems. Reactor systems and analytical techniques have evolved to study the molecular chemistry and microbial ecology within biofilm layers only tens of micrometers thick, and ...

journal_title：Current opinion in biotechnology

authors： Bryers JD

更新日期：1993-04-01 00:00:00

abstract：:Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound protein without using harsh or denaturing elution conditions. The development of methods to identify monoclonal antibodies that bind antigens tightly, but rele...

journal_title：Current opinion in biotechnology

authors： Burgess RR,Thompson NE

更新日期：2002-08-01 00:00:00

abstract：:The worldwide emergence of nanoscale science and engineering was marked by the announcement of the National Nanotechnology Initiative (NNI) in January 2000. Recent research on biosystems at the nanoscale has created one of the most dynamic science and technology domains at the confluence of physical sciences, molecula...

journal_title：Current opinion in biotechnology

authors： Roco MC

更新日期：2003-06-01 00:00:00

abstract：:In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these display techniques ha...

journal_title：Current opinion in biotechnology

authors： Amstutz P,Forrer P,Zahnd C,Plückthun A

更新日期：2001-08-01 00:00:00

abstract：:Significant new advances at the molecular level in the field of plant-pathogen interactions form the basis for novel transgenic approaches to crop protection. The cloning of disease resistance genes and the dissection of the signal transduction components of the hypersensitive response and systemic acquired resistance...

journal_title：Current opinion in biotechnology

authors： Shah DM

更新日期：1997-04-01 00:00:00