Novel methods for cloning and engineering genes using the polymerase chain reaction.

Abstract:

:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (termed UDG cloning) provides an efficient method for gene cloning and recombinant PCR. This technology is now being applied to site-directed mutagenesis, the generation of nested deletions, and the engineering of novel gene constructs. The ease and flexibility of this methodology, combined with PCR amplification, simplify gene cloning and engineering techniques.

journal_name

Curr Opin Biotechnol

authors

Rashtchian A

doi

10.1016/0958-1669(95)80006-9

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

30-6

issue

1

eissn

0958-1669

issn

1879-0429

pii

0958-1669(95)80006-9

journal_volume

6

pub_type

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