Plastid biotechnology: prospects for herbicide and insect resistance, metabolic engineering and molecular farming.


:Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an environmentally benign method of plant genetic engineering, because plastids and their genetic information are maternally inherited in most crops and thus are largely excluded from pollen transmission. During the past few years, researchers in both the public and private sectors have begun to explore possible areas of application of plastid transformation in plant biotechnology as a viable alternative to conventional nuclear transgenic technologies. Recent proof-of-concept studies highlight the potential of plastid genome engineering for the expression of resistance traits, the production of biopharmaceuticals and metabolic pathway engineering in plants.


Curr Opin Biotechnol


Bock R




Has Abstract


2007-04-01 00:00:00














  • Current strategies for cell delivery in cartilage and bone regeneration.

    abstract::Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Sittinger M,Hutmacher DW,Risbud MV

    更新日期:2004-10-01 00:00:00

  • The microbial diversity of inland waters.

    abstract::The conservation and sustainable use of freshwater resources is of global importance. Microorganisms are not only the most abundant organisms in natural freshwater systems, but are also key players in ecological processes controlling water quality. Detailed knowledge of the diversity and function of microorganisms dwe...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hahn MW

    更新日期:2006-06-01 00:00:00

  • Biological approaches for controlling shellfish-associated pathogens.

    abstract::As the consumption of seafood and shellfish increases around the world, so is the incidence of associated outbreaks of illness. Various postharvest treatments are effective at killing seafood-associated bacteria, but most of these treatments also kill the mollusks. Because consumer preferences for raw live shellfish p...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Teplitski M,Wright AC,Lorca G

    更新日期:2009-04-01 00:00:00

  • Transcription factor-based biosensors and inducible systems in non-model bacteria: current progress and future directions.

    abstract::Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kim NM,Sinnott RW,Sandoval NR

    更新日期:2020-08-01 00:00:00

  • Technical issues in the large-scale hydrothermal liquefaction of microalgal biomass to biocrude.

    abstract::Much of the current knowledge on the hydrothermic liquefaction of biomass to biocrude is on the basis of laboratory benchtop findings, and the step up to industrial scale reactors will require a range of information that is currently either unavailable or insufficient. This work highlights a number of these issues suc...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lee A,Lewis D,Kalaitzidis T,Ashman P

    更新日期:2016-04-01 00:00:00

  • Bio-inspired engineering of thiopeptide antibiotics advances the expansion of molecular diversity and utility.

    abstract::Thiopeptide antibiotics, which are a class of sulfur-rich and highly modified peptide natural products, exhibit a wide variety of important biological properties. These antibiotics are ribosomally synthesized and arise from post-translational modifications, exemplifying a process through which nature develops the stru...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lin Z,He Q,Liu W

    更新日期:2017-12-01 00:00:00

  • Isothermal titration calorimetry for characterization of recombinant proteins.

    abstract::Isothermal titration calorimetry is widely used to measure the affinities and enthalpies of interaction between proteins and/or small molecules. The quantitative nature of the technique is especially useful in the characterization of recombinant proteins while determining the fraction of protein capable of binding a s...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Baranauskiene L,Kuo TC,Chen WY,Matulis D

    更新日期:2019-02-01 00:00:00

  • Studying transcriptional interactions in single cells at sufficient resolution.

    abstract::Our ability to dissect and understand the principles of gene regulatory circuits is partly limited by the resolution of our experimental assays. In this brief review, we discuss aspects of gene expression in microbial organisms apparent only when increasing the experimental resolution from populations to single cells ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wallden M,Elf J

    更新日期:2011-02-01 00:00:00

  • RNA as a drug target: chemical, modelling, and evolutionary tools.

    abstract::Dramatic technical progress in RNA synthesis and structure determination has allowed several difficulties inherent to the preparation, handling and structural analysis of RNA to be overcome, and this has led to a wealth of information about RNA structure and its relationship with biological function. It is now fully r...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hermann T,Westhof E

    更新日期:1998-02-01 00:00:00

  • Growth factors as wound healing agents.

    abstract::Wound healing can no longer be considered a generic term: it is the summation of a complex series of processes beginning with coagulation and ending in remodelling. This review, therefore, summarizes the past year's advances in growth factor research according to the roles of the individual growth factors in the speci...

    journal_title:Current opinion in biotechnology

    pub_type: 临床试验,杂志文章,评审


    authors: Robson MC

    更新日期:1991-12-01 00:00:00

  • Ribozymes.

    abstract::RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Rossi JJ

    更新日期:1992-02-01 00:00:00

  • Globin gene switching: a paradigm or what?

    abstract::The delineation of the beta-globin locus control region has led to a new understanding of the developmental regulation of the beta-globin gene cluster. It now seems that globin gene switching is effected through the sequential and mutually exclusive interaction of the locus control region with the embryonic, fetal and...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Enver T,Greaves DR

    更新日期:1991-12-01 00:00:00

  • Protein interaction databases.

    abstract::Life depends on the interaction of proteins. The availability of the complete human genome sequence has highlighted the need for a tool to analyse protein interactions and several databases have been compiled for this purpose. These databases document, categorize, and analyze interacting proteins and the cellular func...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Xenarios I,Eisenberg D

    更新日期:2001-08-01 00:00:00

  • Stem cell niche engineering through droplet microfluidics.

    abstract::Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are bei...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Allazetta S,Lutolf MP

    更新日期:2015-12-01 00:00:00

  • Total internal reflection fluorescence microscopy: technical innovations and novel applications.

    abstract::Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Schneckenburger H

    更新日期:2005-02-01 00:00:00

  • Static and dynamic light scattering of biological macromolecules: what can we learn?

    abstract::Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Murphy RM

    更新日期:1997-02-01 00:00:00

  • Strategies for extended serum half-life of protein therapeutics.

    abstract::With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kontermann RE

    更新日期:2011-12-01 00:00:00

  • Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project.

    abstract::The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet ke...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Parinov S,Sundaresan V

    更新日期:2000-04-01 00:00:00

  • Protein engineering for molecular electronics.

    abstract::Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Sligar SG,Salemme FR

    更新日期:1992-08-01 00:00:00

  • Opportunities toward hydrogen production biotechnologies.

    abstract::Hydrogen is already a major commodity and process intermediate for fertilizer production, petroleum processing, and chemical synthesis. It also offers unrealized potential for energy storage. While biological production offers an expandable and sustainable source, enthusiasm has been dampened by slow research progress...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Swartz J

    更新日期:2020-04-01 00:00:00

  • Application of state-of-art sequencing technologies to indigenous food fermentations.

    abstract::Fermented foods and beverages are an integral part of the human diet globally. Understanding the microbial interactions within these fermenting ecosystems is required to deliver safe products with desirable consumer properties, and moreover, maintenance of these traditions. Effective tools are required for documentati...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: van Hijum SA,Vaughan EE,Vogel RF

    更新日期:2013-04-01 00:00:00

  • Functional foods and strategies contrasting bacterial adhesion.

    abstract::Antibacterial strategies targeting bacterial adhesion to substrates are considered a valuable alternative to traditional antibiotic therapy, in view of the great advantage they bring in combating the infectious process at the very early stage without selecting for drug resistant cells. Amongst bioactive compounds with...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Signoretto C,Canepari P,Stauder M,Vezzulli L,Pruzzo C

    更新日期:2012-04-01 00:00:00

  • Nature's recyclers: anaerobic microbial communities drive crude biomass deconstruction.

    abstract::Microbial communities within anaerobic ecosystems have evolved to degrade and recycle carbon throughout the earth. A number of strains have been isolated from anaerobic microbial communities, which are rich in carbohydrate active enzymes (CAZymes) to liberate fermentable sugars from crude plant biomass (lignocellulose...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lillington SP,Leggieri PA,Heom KA,O'Malley MA

    更新日期:2020-04-01 00:00:00

  • Bioremedial potential of microbial mechanisms of metal mobilization and immobilization.

    abstract::Microorganisms play important roles in the environmental fate of toxic metals and radionuclides with a multiplicity of mechanisms effecting transformations between soluble and insoluble forms. These mechanisms are integral components of natural biogeochemical cycles and are of potential for both in situ and ex situ bi...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Gadd GM

    更新日期:2000-06-01 00:00:00

  • Stable-isotope probing of DNA: insights into the function of uncultivated microorganisms from isotopically labeled metagenomes.

    abstract::Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Friedrich MW

    更新日期:2006-02-01 00:00:00

  • The microbe electric: conversion of organic matter to electricity.

    abstract::Broad application of microbial fuel cells will require substantial increases in current density. A better understanding of the microbiology of these systems may help. Recent studies have greatly expanded the range of microorganisms known to function either as electrode-reducing microorganisms at the anode or as electr...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lovley DR

    更新日期:2008-12-01 00:00:00

  • Silent constraints: the hidden challenges faced in plant metabolic engineering.

    abstract::Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lynch JH,Huang XQ,Dudareva N

    更新日期:2021-01-08 00:00:00

  • Plant secretory structures: more than just reaction bags.

    abstract::Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Tissier A

    更新日期:2018-02-01 00:00:00

  • DNA nanotechnology with one-dimensional self-assembled nanostructures.

    abstract::The information encoded in the base sequence of DNA provides substantial structural and functional information for the instructive self-assembly of one-dimensional (1D) functional DNA nanostructures. The hybridization chain reaction (HCR) and the formation of HCR-stimulated DNAzyme nanochains are presented, as a means...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wang F,Willner B,Willner I

    更新日期:2013-08-01 00:00:00

  • The evolution of IncP catabolic plasmids.

    abstract::The recent adoption of whole plasmid genome sequencing as a routine analytical technique has provided the basis for cataloging the historical events through which plasmids are assembled from the available families of modular plasmid components. Horizontal gene transfer mediated by plasmids plays an important role in t...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Dennis JJ

    更新日期:2005-06-01 00:00:00