Plastid biotechnology: prospects for herbicide and insect resistance, metabolic engineering and molecular farming.

abstract：:Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgen...

journal_title：Current opinion in biotechnology

authors： Somers DA,Makarevitch I

更新日期：2004-04-01 00:00:00

abstract：:Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are bei...

journal_title：Current opinion in biotechnology

authors： Allazetta S,Lutolf MP

更新日期：2015-12-01 00:00:00

abstract：:Biofilms are structured communities of bacteria, which are adhered to a surface and embedded in a self-produced matrix of extracellular polymeric substances. Since biofilms are very resistant to antimicrobial agents, they are at the basis of a range of problems, including quality and safety issues in food industry. Re...

journal_title：Current opinion in biotechnology

authors： Abee T,Kovács AT,Kuipers OP,van der Veen S

更新日期：2011-04-01 00:00:00

abstract：:Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for preci...

journal_title：Current opinion in biotechnology

authors： Tolle F,Stücheli P,Fussenegger M

更新日期：2019-10-01 00:00:00

abstract：:The possibility of generating electricity with microbial fuel cells has been recognized for some time, but practical applications have been slow to develop. The recent development of a microbial fuel cell that can harvest electricity from the organic matter stored in marine sediments has demonstrated the feasibility o...

journal_title：Current opinion in biotechnology

authors： Lovley DR

更新日期：2006-06-01 00:00:00

abstract：:Redox enzymes in bioelectronic devices usually lack direct electrical contact with electrodes, owing to the spatial separation of their redox centers from the conductive surfaces by the protein shells. The reconstitution of apo-enzymes on cofactor-functionalized nanostructures associated with electrodes provides a mea...

journal_title：Current opinion in biotechnology

authors： Willner B,Katz E,Willner I

更新日期：2006-12-01 00:00:00

abstract：:The organization of biochemical networks that make up the living cell can be defined by studying the dynamics of protein-protein interactions. To this end, experimental strategies based on protein fragment complementation assays (PCAs) have been used to map biochemical networks and to identify novel components of thes...

journal_title：Current opinion in biotechnology

authors： Michnick SW

更新日期：2003-12-01 00:00:00

abstract：:Is structure, rather than sequence, the key to the successful generation of truly novel proteins? While protein evolution by homologous recombination has become an established tool to explore confined regions in sequence space, the generation of functional hybrid proteins by homology-independent methods further expand...

journal_title：Current opinion in biotechnology

authors： Lutz S,Benkovic SJ

更新日期：2000-08-01 00:00:00

abstract：:Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes. The number of molecules identified and characterized, however, is in clear opposition to the number of molecules that are ente...

journal_title：Current opinion in biotechnology

authors： Maurer KH

更新日期：2004-08-01 00:00:00

abstract：:Antisense technology is finding increasing application not only in clinical development, but also for cellular engineering. Several types of antisense methods (e.g. antisense oligonucleotides, antisense RNA and small interfering RNA) can be used to inhibit the expression of a target gene. These antisense methods are b...

journal_title：Current opinion in biotechnology

authors： Lee LK,Roth CM

更新日期：2003-10-01 00:00:00

abstract：:The fundamental role of glycoconjugates in many biological processes is now well appreciated and has intensified the development of innovative and improved synthetic strategies. All areas of synthetic methodology have seen major advances and many complex, highly branched carbohydrates and glycoproteins have been prepa...

journal_title：Current opinion in biotechnology

authors： Hölemann A,Seeberger PH

更新日期：2004-12-01 00:00:00

abstract：:Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...

journal_title：Current opinion in biotechnology

authors： Park D,Swayambhu G,Pfeifer BA

更新日期：2020-12-01 00:00:00

abstract：:Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent...

journal_title：Current opinion in biotechnology

authors： Stoloff DH,Wanunu M

更新日期：2013-08-01 00:00:00

abstract：:The biosynthesis of the solvents 1-butanol and acetone is restricted to species of the genus Clostridium, a diverse group of Gram-positive, endospore forming anaerobes comprising toxin-producing strains as well as terrestrial non-pathogenic species of biotechnological impact. Among solventogenic clostridia, Clostridiu...

journal_title：Current opinion in biotechnology

authors： Lütke-Eversloh T,Bahl H

更新日期：2011-10-01 00:00:00

abstract：:Fundamental biological processes such as cell-cell communication, signal transduction, molecular transport and energy conversion are performed by membrane proteins. These important proteins are studied best in their native environment, the lipid bilayer. The atomic force microscope (AFM) is the instrument of choice to...

journal_title：Current opinion in biotechnology

authors： Fotiadis D

更新日期：2012-08-01 00:00:00

abstract：:Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...

journal_title：Current opinion in biotechnology

authors： Marquardt LM,Sakiyama-Elbert SE

更新日期：2013-10-01 00:00:00

abstract：:Global aquaculture is projected to grow from 10 million to 20 million metric tons by the year 2000. To meet this projection, the aquaculture industry must bring some of its infectious disease problems under control without relying on antibiotics and chemotherapeutics. Thus, vaccines for fish and shellfish are being so...

journal_title：Current opinion in biotechnology

authors： Leong JA

更新日期：1993-06-01 00:00:00

abstract：:Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...

journal_title：Current opinion in biotechnology

authors： Rehemtulla A,Kaufman RJ

更新日期：1992-10-01 00:00:00

abstract：:Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

journal_title：Current opinion in biotechnology

authors： Junker BH

更新日期：2014-04-01 00:00:00

abstract：:In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing to predict the expr...

journal_title：Current opinion in biotechnology

authors： Wirth D,Gama-Norton L,Riemer P,Sandhu U,Schucht R,Hauser H

更新日期：2007-10-01 00:00:00

abstract：:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...

journal_title：Current opinion in biotechnology

authors： Vervoort Y,Linares AG,Roncoroni M,Liu C,Steensels J,Verstrepen KJ

更新日期：2017-08-01 00:00:00

abstract：:An intense interest has grown in the unique interactions of nucleic acids with metal ions, which lead to the formation of metal-base pairs and the generation of fluorescent nanomaterials. In this review, different types of metal-base pairs, especially those formed from naturally occurring nucleosides, are described wi...

journal_title：Current opinion in biotechnology

authors： Park KS,Park HG

更新日期：2014-08-01 00:00:00

abstract：:The selection of an expression platform for recombinant biopharmaceuticals is often centered upon suitable product titers and critical quality attributes, including post-translational modifications. Although notable differences between microbial, yeast, plant, and mammalian host systems exist, recent advances have gre...

journal_title：Current opinion in biotechnology

authors： Meehl MA,Stadheim TA

更新日期：2014-12-01 00:00:00

abstract：:Escherichia coli is still a very popular host for the production of recombinant proteins at an analytical or industrial scale. Secretion of the proteins into the culture medium or display at the cell surface would be preferred in many applications but is hampered by the complex two-layered cell envelope. The autotrans...

journal_title：Current opinion in biotechnology

authors： Jong WS,Saurí A,Luirink J

更新日期：2010-10-01 00:00:00

abstract：:Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...

journal_title：Current opinion in biotechnology

authors： DelRosso NV,Derr ND

更新日期：2017-08-01 00:00:00

abstract：:Herbicides are economically important, but the non-point pollution that they cause may disrupt the surrounding environment. Phytoremediation of herbicides has been well studied using conventional plants. Transgenic plants produced for metabolizing herbicides and long-persisting pollutants can be used for phytoremediat...

journal_title：Current opinion in biotechnology

authors： Kawahigashi H

更新日期：2009-04-01 00:00:00

abstract：:Contamination of the vadose zone with various pollutants is a world-wide problem, and often technical or economic constraints impose remediation without excavation. In situ bioremediation in the vadose zone by bioventing has become a standard remediation technology for light spilled petroleum products. In this review,...

journal_title：Current opinion in biotechnology

authors： Höhener P,Ponsin V

更新日期：2014-06-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:Metabolic engineering is not another form of classic manipulation of intermediary metabolism; rather, it is the purposeful design of metabolic networks. The metabolic engineering approach examines biochemical reactions in their entirety, rather than individually, and is concerned with the construction of novel pathway...

journal_title：Current opinion in biotechnology

authors： Stephanopoulos G

更新日期：1994-02-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00