Ultrafast laser nanosurgery in microfluidics for genome-wide screenings.

Abstract:

:The use of ultrafast laser pulses in surgery has allowed for unprecedented precision with minimal collateral damage to surrounding tissues. For these reasons, ultrafast laser nanosurgery, as an injury model, has gained tremendous momentum in experimental biology ranging from in vitro manipulations of subcellular structures to in vivo studies in whole living organisms. For example, femtosecond laser nanosurgery on such model organism as the nematode Caenorhabditis elegans has opened new opportunities for in vivo nerve regeneration studies. Meanwhile, the development of novel microfluidic devices has brought the control in experimental environment to the level required for precise nanosurgery in various animal models. Merging microfluidics and laser nanosurgery has recently improved the specificities and increased the speed of laser surgeries enabling fast genome-wide screenings that can more readily decode the genetic map of various biological processes.

journal_name

Curr Opin Biotechnol

authors

Ben-Yakar A,Bourgeois F

doi

10.1016/j.copbio.2009.01.008

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

100-5

issue

1

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(09)00010-X

journal_volume

20

pub_type

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