Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli.

abstract：:The ecophysiology of microorganisms has been at the heart of microbial ecology since its early days, but only during the past decade have methods become available for cultivation-independent, direct identification of microorganisms in complex communities and for the simultaneous investigation of their activity and sub...

journal_title：Current opinion in biotechnology

authors： Wagner M,Nielsen PH,Loy A,Nielsen JL,Daims H

更新日期：2006-02-01 00:00:00

abstract：:Advances in the separation of microbial subpopulations and in proteomics technologies have paved the way for the global molecular characterization of microbial cells that share common functional characteristics. Quantitative characterization of the dynamics of microbial proteomes enables an unprecedented view of the a...

journal_title：Current opinion in biotechnology

authors： Bernhardt J,Michalik S,Wollscheid B,Völker U,Schmidt F

更新日期：2013-02-01 00:00:00

abstract：:Generating novel functionality from well characterised synthetic parts and modules lies at the heart of synthetic biology. Ideally, circuitry is rationally designed in silico with quantitatively predictive models to predetermined design specifications. Synthetic circuits are intrinsically stochastic, often dynamically...

journal_title：Current opinion in biotechnology

authors： Prangemeier T,Lehr FX,Schoeman RM,Koeppl H

更新日期：2020-06-01 00:00:00

abstract：:Gene expression control is critical to increase production of recombinant proteins, fine-tune metabolic pathways and reliably express synthetic pathways. The importance of transcriptional control seems to be most important in eukaryotic systems. In this review, we highlight recent developments in the field of transcri...

journal_title：Current opinion in biotechnology

authors： Leavitt JM,Alper HS

更新日期：2015-08-01 00:00:00

abstract：:The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...

journal_title：Current opinion in biotechnology

authors： Luna RA,Foster JA

更新日期：2015-04-01 00:00:00

abstract：:Understanding the hierarchical structure of proteins at their fundamental length scales is essential to get insights into their functions and roles in fundamental biological processes. Near-field scanning optical microscopy (NSOM), which overcomes the diffraction limits of conventional optics, provides a powerful anal...

journal_title：Current opinion in biotechnology

authors： Yong YC,Wang YZ,Zhong JJ

更新日期：2018-12-01 00:00:00

abstract：:Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

journal_title：Current opinion in biotechnology

authors： Meyer RD,Cook D

更新日期：2000-02-01 00:00:00

abstract：:The use of microbial consortia for bioprocessing has been limited by our ability to reliably control community composition and function simultaneously. Recent advances in synthetic biology have enabled population-level coordination and control of ecosystem stability and dynamics. Further, new experimental and computat...

journal_title：Current opinion in biotechnology

authors： Shong J,Jimenez Diaz MR,Collins CH

更新日期：2012-10-01 00:00:00

abstract：:Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...

journal_title：Current opinion in biotechnology

authors： Rehemtulla A,Kaufman RJ

更新日期：1992-10-01 00:00:00

abstract：:Intraportal islet transplantation has emerged as a promising treatment for type 1 diabetes mellitus (T1DM). Nevertheless, long-term efficacy has been limited to a marginal number of patients. Outcomes have been restricted, in part, by challenges associated with the transplant site, poor vascularization, and disruption...

journal_title：Current opinion in biotechnology

authors： Coronel MM,Stabler CL

更新日期：2013-10-01 00:00:00

abstract：:Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding ...

journal_title：Current opinion in biotechnology

authors： Lynch JH,Huang XQ,Dudareva N

更新日期：2021-01-08 00:00:00

abstract：:The design and construction of synthetic gene circuits with complex spatiotemporal dynamics was pioneered in bacteria, but it took almost a decade until synthetic biologists were able to construct genetic circuits with complex spatiotemporal dynamics in mammalian cells. This review highlights the most recent advances ...

journal_title：Current opinion in biotechnology

authors： Weber W,Fussenegger M

更新日期：2010-10-01 00:00:00

abstract：:Random peptide libraries displayed on phage are used as a source of peptides for epitope mapping, for the identification of critical amino acids responsible for protein-protein interactions and as leads for the discovery of new therapeutics. Efficient and simple procedures have been devised to select peptides binding ...

journal_title：Current opinion in biotechnology

authors： Cortese R,Monaci P,Luzzago A,Santini C,Bartoli F,Cortese I,Fortugno P,Galfrè G,Nicosia A,Felici F

更新日期：1996-12-01 00:00:00

abstract：:Biological pulping has the potential to improve the quality of pulp, properties of paper and to reduce energy costs and environmental impact relative to traditional pulping operations. It has been suggested that energy savings alone could make the process economically viable. Other benefits include improved burst stre...

journal_title：Current opinion in biotechnology

authors： Breen A,Singleton FL

更新日期：1999-06-01 00:00:00

abstract：:The spatiotemporal patterns of ion and metabolite levels in living cells are important in understanding signal transduction and metabolite flux. Imaging approaches using genetically encoded sensors are ideal for detecting such molecule dynamics, which are hard to capture otherwise. Recent years have seen iterative imp...

journal_title：Current opinion in biotechnology

authors： Okumoto S

更新日期：2010-02-01 00:00:00

abstract：:Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applicat...

journal_title：Current opinion in biotechnology

authors： Pfeiffer F,Rosenthal M,Siegl J,Ewers J,Mayer G

更新日期：2017-12-01 00:00:00

abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

journal_title：Current opinion in biotechnology

authors： Pais DA,Carrondo MJ,Alves PM,Teixeira AP

更新日期：2014-12-01 00:00:00

abstract：:As bacterial resistance to currently used antibiotics increases, so too must efforts to identify novel agents and strategies for the prevention and treatment of bacterial infection. In the past, antimicrobial drug discovery efforts have focused on eradicating infection by either cidal or static agents, resulting in cl...

journal_title：Current opinion in biotechnology

authors： Alksne LE,Projan SJ

更新日期：2000-12-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:Both prokaryotic and eukaryotic cells respond to the accumulation of unfolded proteins by increasing the transcription of genes encoding molecular chaperones and other stress-responsive proteins. Different sets of genes are activated when particular cellular compartments are burdened with unfolded proteins. Cells thus...

journal_title：Current opinion in biotechnology

authors： McMillan DR,Gething MJ,Sambrook J

更新日期：1994-10-01 00:00:00

abstract：:Mismatch-repair systems have been identified in organisms ranging from Escherichia coli to humans. They can repair almost all DNA base pair mismatches as well as small insertion/deletion mismatches. Molecular and biochemical analyses have shown that the core components of eukaryotic mismatch-repair systems are highly ...

journal_title：Current opinion in biotechnology

authors： Kolodner RD,Alani E

更新日期：1994-12-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00

abstract：:Fatty acid oxidation pathways are attractive for metabolic engineering purposes due to their cyclic nature as well as their reactions that allow for the selective functionalization of alkyl chains. These characteristics allow for the production of various chemicals, such as alcohols, alkanes, ketones and hydroxyacids,...

journal_title：Current opinion in biotechnology

authors： Kim S,Cheong S,Chou A,Gonzalez R

更新日期：2016-12-01 00:00:00

abstract：:A mechanistic understanding of gene regulatory network dynamics requires quantitative single-cell data of multiple network components in response to well-defined perturbations. Recent advances in the development of fluorescent biomarkers for proteins, detection of RNA and interactions, microfluidic technology, and hig...

journal_title：Current opinion in biotechnology

authors： Selimkhanov J,Hasty J,Tsimring LS

更新日期：2012-02-01 00:00:00

abstract：:Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...

journal_title：Current opinion in biotechnology

authors： Labrou NE,Papageorgiou AC,Pavli O,Flemetakis E

更新日期：2015-04-01 00:00:00

abstract：:Studies of biological systems in which there is a direct link between the challenges faced by marine organisms and biotechnologies enable us to rationally search for active natural compounds and other novel biotechnologies. This approach is proving successful in developing new methods for the prevention of marine biof...

journal_title：Current opinion in biotechnology

authors： de Nys R,Steinberg PD

更新日期：2002-06-01 00:00:00

abstract：:In the past year, significant progress in the field of gene transfer has been made possible by refinement of the technique of particle bombardment. The process has been utilized for the study of gene expression in plastids and mitochondria, the production of transgenic crop plants and gene transfer into live animals. ...

journal_title：Current opinion in biotechnology

authors： Klein TM,Fitzpatrick-McElligott S

更新日期：1993-10-01 00:00:00

abstract：:Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1-200 microm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit inc...

journal_title：Current opinion in biotechnology

authors： Govardhan CP

更新日期：1999-08-01 00:00:00

abstract：:The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

journal_title：Current opinion in biotechnology

authors： Tao WA,Aebersold R

更新日期：2003-02-01 00:00:00