Abstract：
:Hydrogen is already a major commodity and process intermediate for fertilizer production, petroleum processing, and chemical synthesis. It also offers unrealized potential for energy storage. While biological production offers an expandable and sustainable source, enthusiasm has been dampened by slow research progress. Also, the very low cost of natural gas (the major current hydrogen source) imposes severe economic challenges. This discussion describes process, metabolic, and protein engineering opportunities toward cost-effective biohydrogen production. Recent progress in hydrogenase engineering and photosynthetic bacterial research now suggests a favorable risk versus reward opportunity. Although the risks are still significant, successful technologies would provide important components in an integrated energy portfolio that enables global sustainability.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Swartz Jdoi
10.1016/j.copbio.2020.03.002subject
Has Abstractpub_date
2020-04-01 00:00:00pages
248-255eissn
0958-1669issn
1879-0429pii
S0958-1669(20)30039-2journal_volume
62pub_type
杂志文章,评审abstract：:Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2020.06.014
更新日期：2020-12-01 00:00:00
abstract：:Transgenic experimentation has become a crucial part of hypertension and atherosclerosis research, and is growing more important in several other areas of cardiovascular disease. It has recently made a particular contribution to understanding the role of the renin-angiotensin system in controlling hypertension. The st...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(92)90008-7
更新日期：1992-12-01 00:00:00
abstract：:The metabolome, the collection of small molecules associated with an organism, is a growing subject of inquiry, with the data utilized for data-intensive systems biology, disease diagnostics, biomarker discovery, and the broader characterization of small molecules in mixtures. Owing to their close proximity to the fun...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.08.005
更新日期：2017-02-01 00:00:00
abstract：:New methods and applications in the separation of biomolecules are reviewed, with an emphasis on the large-scale recovery of proteins. Highlights include the advent of flow-through particles in perfusion chromatography, which allows for very high flow rates, while retaining a high chromatographic efficiency. ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(05)80148-x
更新日期：1991-06-01 00:00:00
abstract：:Intraportal islet transplantation has emerged as a promising treatment for type 1 diabetes mellitus (T1DM). Nevertheless, long-term efficacy has been limited to a marginal number of patients. Outcomes have been restricted, in part, by challenges associated with the transplant site, poor vascularization, and disruption...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2013.05.004
更新日期：2013-10-01 00:00:00
abstract：:Significant new advances at the molecular level in the field of plant-pathogen interactions form the basis for novel transgenic approaches to crop protection. The cloning of disease resistance genes and the dissection of the signal transduction components of the hypersensitive response and systemic acquired resistance...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/s0958-1669(97)80104-8
更新日期：1997-04-01 00:00:00
abstract：:The world continues to increase its energy use, brought about by an expanding population and a desire for a greater standard of living. This energy use coupled with the realization of the impact of carbon dioxide on the climate, has led us to reanalyze the potential of plant-based biofuels. Of the potential sources of...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.10.013
更新日期：2012-06-01 00:00:00
abstract：:Metabolomics, as an essential part of genomics studies, intends holistic understanding of metabolic networks through simultaneous analysis of a myriad of both known and unknown metabolites occurring in living organisms. The initial stage of metabolomics was designed for the reproducible analyses of known metabolites b...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章
doi：10.1016/j.copbio.2010.01.012
更新日期：2010-02-01 00:00:00
abstract：:Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understa...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2009.08.005
更新日期：2009-08-01 00:00:00
abstract：:The ecophysiology of microorganisms has been at the heart of microbial ecology since its early days, but only during the past decade have methods become available for cultivation-independent, direct identification of microorganisms in complex communities and for the simultaneous investigation of their activity and sub...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2005.12.006
更新日期：2006-02-01 00:00:00
abstract：:Recombinant proteins that have survived the challenges of process development and clinical trials are becoming blockbuster medical products. Growth factors, enzymes and antibodies are being improved by mutational approaches, fused with other proteins, and even chemically modified in vitro. Drug development and testing...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(95)80112-x
更新日期：1995-12-01 00:00:00
abstract：:Adaptive laboratory evolution (ALE) strategies allow for the metabolic engineering of microorganisms by combining genetic variation with the selection of beneficial mutations in an unbiased fashion. These ALE strategies have been proven highly effective in the optimization of production strains. In contrast to rationa...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.03.007
更新日期：2011-08-01 00:00:00
abstract：:Estrogen receptors are multi-domain proteins that interact with other proteins and DNA to fulfil their function: the regulation of transcription. During the past 2-3 years, our understanding of this complex process has increased tremendously as crystal structures of isolated ligand-binding domains in complex with vari...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(99)00034-8
更新日期：1999-12-01 00:00:00
abstract：:Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(00)00077-x
更新日期：2000-04-01 00:00:00
abstract：:Various strategies have been developed to exploit plants as bioreactors for the production of pharmaceutical antibodies, to engineer antibody-mediated pathogen resistance or to alter the plant phenotype by immunomodulation. Recent research developments focus on the fine-tuning of expression systems and the detailed ch...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(02)00303-8
更新日期：2002-04-01 00:00:00
abstract：:Extremolytes are small organic molecules, which protect cells under extreme, virtually inhabitable conditions. Their exceptional properties can be translated into health-promoting and therapeutic activities, which open an avenue of opportunities for the cosmetic, medical, and food industries. Supported by powerful app...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2020.02.010
更新日期：2020-10-01 00:00:00
abstract：:Scaffolds are used in tissue engineering as a matrix for the seeding and attachment of human cells. The creation of porosity in three-dimensional (3D) structures of scaffolds plays a critical role in cell proliferation, migration, and differentiation into the specific tissue while secreting extracellular matrix compon...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.04.005
更新日期：2011-10-01 00:00:00
abstract：:RNA imaging probes help us investigate how transport and dynamics of RNA contribute to subcellular RNA localization or regulation of gene expression. Out of the plethora of strategies that have been developed to image RNA in living cells, genetically encoded probes are interesting because they can be produced by the c...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.07.012
更新日期：2015-02-01 00:00:00
abstract：:Combinatorial biocatalysis is an emerging technology in the field of drug discovery. The biocatalytic approach to combinatorial chemistry uses enzymatic, chemoenzymatic, and microbial transformations to generate libraries from lead compounds. Important recent advances in combinatorial biocatalysis include iterative de...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0958-1669(99)80022-6
更新日期：1999-04-01 00:00:00
abstract：:A growing body of evidence indicates that many cellular reactions within metabolic pathways are catalyzed not by free-floating 'soluble' enzymes, but via one or more membrane-associated multienzyme complexes. This type of macromolecular organization has important implications for the overall efficiency, specificity, a...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2008.07.006
更新日期：2008-10-01 00:00:00
abstract：:Marine microbes have received growing attention as sources of bioactive metabolites and offer a unique opportunity to both increase the number of marine natural products in clinical trials as well as expedite their development. This review focuses specifically on those molecules currently in the clinical pipeline that...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2010.09.013
更新日期：2010-12-01 00:00:00
abstract：:Many significant advances have occurred recently in the field of protein electrophoresis and related technologies. Improvements have been made in capillary electrophoresis in apparatus design, detection, and capillary modification with coatings and fillers. The transfer of proteins to a blot, the capacity to analyze a...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0958-1669(93)90024-q
更新日期：1993-02-01 00:00:00
abstract：:In recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. It has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ESTs had limited impact for breeding. Until recently, the models used by ...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2011.09.002
更新日期：2012-04-01 00:00:00
abstract：:Fibrotic diseases occur in virtually every tissue of the body and are a major cause of mortality, yet they remain largely untreatable and poorly understood on a mechanistic level. The development of anti-fibrotic agents has been hampered, in part, by the insufficient fibrosis biomimicry provided by traditional in vitr...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2016.02.006
更新日期：2016-08-01 00:00:00
abstract：:Vernalization is the process by which flowering is promoted by prolonged exposure to the cold of a typical winter. In certain plant species, the role of vernalization is to suppress the expression of genes that encode repressors of flowering. In Arabidopsis, this suppression is an epigenetic phenomenon in the sense th...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2005.02.004
更新日期：2005-04-01 00:00:00
abstract：:The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2014.10.007
更新日期：2015-04-01 00:00:00
abstract：:Advances in the separation of microbial subpopulations and in proteomics technologies have paved the way for the global molecular characterization of microbial cells that share common functional characteristics. Quantitative characterization of the dynamics of microbial proteomes enables an unprecedented view of the a...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.10.009
更新日期：2013-02-01 00:00:00
abstract：:Metabolic flux analysis (MFA) represents a powerful tool for systems biology research on eukaryotic cells. This review describes recent advances, the challenges as well as applications of metabolic flux analysis comprising fungi, mammalian cells and plants. While MFA is widely established and applied in microorganisms...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2010.01.011
更新日期：2010-02-01 00:00:00
abstract：:Efforts to develop culture technologies capable of eliciting robust human blood stem cell growth have met with limited success. Considering that adult stem cell cultures are complex systems, comprising multiple cell types with dynamically changing intracellular signalling environments and cellular compositions, this i...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2006.07.003
更新日期：2006-10-01 00:00:00
abstract：:Fermented foods and beverages are an integral part of the human diet globally. Understanding the microbial interactions within these fermenting ecosystems is required to deliver safe products with desirable consumer properties, and moreover, maintenance of these traditions. Effective tools are required for documentati...
journal_title：Current opinion in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.copbio.2012.08.004
更新日期：2013-04-01 00:00:00
