Abstract:
:Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understanding, directed evolution is a powerful complement to 'rational' engineering approaches. Aided by clever selection schemes, directed evolution has generated new parts for genetic circuits, cell-cell communication systems, and non-natural metabolic pathways in bacteria.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Dougherty MJ,Arnold FHdoi
10.1016/j.copbio.2009.08.005subject
Has Abstractpub_date
2009-08-01 00:00:00pages
486-91issue
4eissn
0958-1669issn
1879-0429pii
S0958-1669(09)00098-6journal_volume
20pub_type
杂志文章,评审abstract::In the past year, significant progress in the field of gene transfer has been made possible by refinement of the technique of particle bombardment. The process has been utilized for the study of gene expression in plastids and mitochondria, the production of transgenic crop plants and gene transfer into live animals. ...
journal_title:Current opinion in biotechnology
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abstract::Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....
journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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abstract::Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an...
journal_title:Current opinion in biotechnology
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abstract::Tumor-derived DNA, found in body fluids (liquid biopsy) of cancer patients as part of cell-free DNA (cfDNA), lends itself for noninvasive cancer detection and monitoring. Advantages of cfDNA as analytical target have evoked a burst of sophisticated techniques, providing clinically relevant information. Each cell type ...
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abstract::It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...
journal_title:Current opinion in biotechnology
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abstract::Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...
journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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更新日期:2015-06-01 00:00:00
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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abstract::Synthetic biology combines knowledge from various disciplines including molecular biology, engineering, mathematics and physics to design and build novel proteins, genetic circuits and metabolic networks. Early efforts aimed at altering the behavior of individual elements have now evolved to focus on the construction ...
journal_title:Current opinion in biotechnology
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abstract::Most secretory proteins are synthesized as precursors with an amino-terminal signal peptide. Genetic identification of proteins essential for signal peptide dependent translocation to the Escherichia coli periplasm has led to the biochemical dissection of the secretion pathway. Additional mechanisms exist in Gram-nega...
journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
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abstract::Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...
journal_title:Current opinion in biotechnology
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