Abstract:
:The ecophysiology of microorganisms has been at the heart of microbial ecology since its early days, but only during the past decade have methods become available for cultivation-independent, direct identification of microorganisms in complex communities and for the simultaneous investigation of their activity and substrate uptake patterns. The combination of fluorescence in situ hybridization (FISH) and microautoradiography (MAR) is currently the most widely applied tool for revealing physiological properties of microorganisms in their natural environment with single-cell resolution. For example, this technique has been used in wastewater treatment and marine systems to describe the functional properties of newly discovered species, and to identify microorganisms responsible for key physiological processes. Recently, the scope of FISH-MAR was extended by rendering it quantitative and by combining it with microelectrode measurements or stable isotope probing. Isotope arrays have also been developed that exploit the parallel detection offered by DNA microarrays to measure incorporation of labelled substrate into the rRNA of many community members in a single experiment.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Wagner M,Nielsen PH,Loy A,Nielsen JL,Daims Hdoi
10.1016/j.copbio.2005.12.006subject
Has Abstractpub_date
2006-02-01 00:00:00pages
83-91issue
1eissn
0958-1669issn
1879-0429pii
S0958-1669(05)00198-9journal_volume
17pub_type
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