The role of sigmaB in the stress response of Gram-positive bacteria -- targets for food preservation and safety.

abstract：:Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of B...

journal_title：Current opinion in biotechnology

authors： Plegaria JS,Kerfeld CA

更新日期：2018-06-01 00:00:00

abstract：:Saccharomyces cerevisiae has decisive advantages in industrial processes due to its tolerance to alcohols and fermentation conditions. Butanol isomers are considered as suitable fuel substitutes and valuable biomass-derived chemical building blocks. Whereas high production was achieved with bacterial systems, metaboli...

journal_title：Current opinion in biotechnology

authors： Generoso WC,Schadeweg V,Oreb M,Boles E

更新日期：2015-06-01 00:00:00

abstract：:The delineation of the beta-globin locus control region has led to a new understanding of the developmental regulation of the beta-globin gene cluster. It now seems that globin gene switching is effected through the sequential and mutually exclusive interaction of the locus control region with the embryonic, fetal and...

journal_title：Current opinion in biotechnology

authors： Enver T,Greaves DR

更新日期：1991-12-01 00:00:00

abstract：:Antibacterial strategies targeting bacterial adhesion to substrates are considered a valuable alternative to traditional antibiotic therapy, in view of the great advantage they bring in combating the infectious process at the very early stage without selecting for drug resistant cells. Amongst bioactive compounds with...

journal_title：Current opinion in biotechnology

authors： Signoretto C,Canepari P,Stauder M,Vezzulli L,Pruzzo C

更新日期：2012-04-01 00:00:00

abstract：:Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel ...

journal_title：Current opinion in biotechnology

authors： Schneckenburger H

更新日期：2005-02-01 00:00:00

abstract：:Synthetic biology combines knowledge from various disciplines including molecular biology, engineering, mathematics and physics to design and build novel proteins, genetic circuits and metabolic networks. Early efforts aimed at altering the behavior of individual elements have now evolved to focus on the construction ...

journal_title：Current opinion in biotechnology

authors： McDaniel R,Weiss R

更新日期：2005-08-01 00:00:00

abstract：:To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...

journal_title：Current opinion in biotechnology

authors： Azerad R,Buisson D

更新日期：2000-12-01 00:00:00

abstract：:The release of sub-picomole levels of N-linked oligosaccharides directly from 1-5 micrograms of protein in a band on an SDS PAGE gel, coupled with recent advances in mass spectrometry and HPLC, opens the way for the analysis of biologically important glycoproteins that are difficult to purify or are available only in ...

journal_title：Current opinion in biotechnology

authors： Rudd PM,Dwek RA

更新日期：1997-08-01 00:00:00

abstract：:The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

journal_title：Current opinion in biotechnology

authors： Tao WA,Aebersold R

更新日期：2003-02-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:Biological pulping has the potential to improve the quality of pulp, properties of paper and to reduce energy costs and environmental impact relative to traditional pulping operations. It has been suggested that energy savings alone could make the process economically viable. Other benefits include improved burst stre...

journal_title：Current opinion in biotechnology

authors： Breen A,Singleton FL

更新日期：1999-06-01 00:00:00

abstract：:Ultrasonographic imaging of ovarian morphology is used widely to inform reproductive health status in women. Metabolic disturbances induced by a negative energy balance (e.g. undernutrition) or positive energy balance (e.g. overnutrition, obesity) are known to drive or exacerbate reproductive dysfunction. Whether the ...

journal_title：Current opinion in biotechnology

authors： Vanden Brink H,Pea J,Lujan ME

更新日期：2020-11-25 00:00:00

abstract：:Although metabolic networks can be readily reconstructed through comparative genomics, the reconstruction of regulatory networks has been hindered by the relatively low level of evolutionary conservation of their molecular components. Recent developments in experimental techniques have allowed the generation of vast a...

journal_title：Current opinion in biotechnology

authors： Herrgård MJ,Covert MW,Palsson BØ

更新日期：2004-02-01 00:00:00

abstract：:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the p...

journal_title：Current opinion in biotechnology

authors： Gygi SP,Rist B,Aebersold R

更新日期：2000-08-01 00:00:00

abstract：:In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these display techniques ha...

journal_title：Current opinion in biotechnology

authors： Amstutz P,Forrer P,Zahnd C,Plückthun A

更新日期：2001-08-01 00:00:00

abstract：:Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hos...

journal_title：Current opinion in biotechnology

authors： Yuzawa S,Kim W,Katz L,Keasling JD

更新日期：2012-10-01 00:00:00

abstract：:Hydrogen is already a major commodity and process intermediate for fertilizer production, petroleum processing, and chemical synthesis. It also offers unrealized potential for energy storage. While biological production offers an expandable and sustainable source, enthusiasm has been dampened by slow research progress...

journal_title：Current opinion in biotechnology

authors： Swartz J

更新日期：2020-04-01 00:00:00

abstract：:Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an...

journal_title：Current opinion in biotechnology

authors： Bock R

更新日期：2007-04-01 00:00:00

abstract：:Bioremediation has historically been approached as a 'black box' in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at t...

journal_title：Current opinion in biotechnology

authors： Chakraborty R,Wu CH,Hazen TC

更新日期：2012-06-01 00:00:00

abstract：:Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

journal_title：Current opinion in biotechnology

authors： Junker BH

更新日期：2014-04-01 00:00:00

abstract：:Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes. The number of molecules identified and characterized, however, is in clear opposition to the number of molecules that are ente...

journal_title：Current opinion in biotechnology

authors： Maurer KH

更新日期：2004-08-01 00:00:00

abstract：:Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...

journal_title：Current opinion in biotechnology

authors： Vanholme B,El Houari I,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:As the ambitions of synthetic biology approach genome-scale engineering, comprehensive characterization of cellular systems is required, as well as a means to accurately model cell-scale molecular interactions. These requirements are coincident with the goals of systems biology and, thus, systems biology will become t...

journal_title：Current opinion in biotechnology

authors： Barrett CL,Kim TY,Kim HU,Palsson BØ,Lee SY

更新日期：2006-10-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:Natural products have demonstrated value across numerous application areas, with antibiotics a notable historical example. Native cellular hosts provide an initial option in efforts to harness natural product production. However, various complexities associated with native hosts, including fastidious growth traits and...

journal_title：Current opinion in biotechnology

authors： Park D,Swayambhu G,Pfeifer BA

更新日期：2020-12-01 00:00:00

abstract：:It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...

journal_title：Current opinion in biotechnology

authors： Kuwajima K

更新日期：1992-10-01 00:00:00

abstract：:Modern agriculture and food production systems are facing increasing pressures from climate change, land and water availability, and, more recently, a pandemic. These factors are threatening the environmental and economic sustainability of current and future food supply systems. Scientific and technological innovation...

journal_title：Current opinion in biotechnology

authors： Jung J,Maeda M,Chang A,Bhandari M,Ashapure A,Landivar-Bowles J

更新日期：2020-10-07 00:00:00

abstract：:Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies...

journal_title：Current opinion in biotechnology

authors： Suter B,Kittanakom S,Stagljar I

更新日期：2008-08-01 00:00:00

abstract：:The demand for validated analytical methods for botanicals has grown in response to the increasing consumer market for botanical supplements. Government initiatives to increase the availability of validated analytical methods and botanical reference material have led to the publication of numerous validation studies i...

journal_title：Current opinion in biotechnology

authors： Brown PN,Lister P

更新日期：2014-02-01 00:00:00